Identification and characterization of a phytase of potential commercial interest

Casey, A. N. J.; Walsh, Gary

doi:10.1016/j.jbiotec.2004.03.001

Cited by 83 publications

(37 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Generally, the Rhizopusderived enzyme exhibits an optimal phytase activity level at 50-60 o C and at a pH of 2.0 to 6.0. These are important values for feed applications and commercial perspectives (5,28). Agricultural feed additives containing phytic acid have caused nutritional deficiencies and higher rates of environmental pollution due to a maximum load of phosphorus excreted by monogastric animals due to lack of a phytic acid degrading enzyme in the gastrointestinal tract (2).…”

Section: Application Of Phytase Using Different Feed Additivesmentioning

confidence: 99%

“…The maximum amount of phytase hydrolytic products was obtained using RF and CF. Carbohydrate rich food grains produced the maximum amounts of phytase hydrolytic products using R. oligosporus phytase, perhaps due to high carbohydrate contents (30.5%) (5). In the presence of a glycosylatic enzyme, the R. oligosporus phytase has been shown to exhibit high catalytic efficiency and stability (30).…”

Section: Application Of Phytase Using Different Feed Additivesmentioning

confidence: 99%

“…Recent studies have focused on phytase production by Rhizopus sp. and phytase from R. oligosporus has been purified and characterized by Casey and Walsh (5). Preference for use of R. oligosporus phytase in animal feed area makes it a promising candidate for phytic acid hydrolysis and commercial application.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of a mixed substrate on phytase production by Rhizopus oligosporus MTCC 556 using solid state fermentation and determination of dephytinization activities in food grains

Suresh

Radha

2015

Food Sci Biotechnol

View full text Add to dashboard Cite

Mustard oilcake was used as a novel substrate for phytase production with Rhizopus oligosporus MTCC 556. A mixed substrate (wheat bran:mustard oil cake) was used to optimize different medium components under solid state fermentation at a 62% moisture content, pH 5.5, 30ºC, an inoculum level of 15%, and a ratio of the volume of a salt solution to the mass of mixed substrate 1.5 mL:10 g. Different carbon and nitrogen sources were used for production of phytase. Use of the nutritional supplements maltose and ammonium sulfate resulted in high phytase yields. The hydrolytic ability of partially purified phytase was evaluated using 5 different food grains. Wheat flour showed a 43.78% phytic acid reduction, rice flour showed 92% release of inorganic phosphorus, and corn flour showed 81 and 68% releases of soluble proteins and reducing sugars. Thus, phytase is suitable for feed applications.

show abstract

Section: Application Of Phytase Using Different Feed Additivesmentioning

confidence: 99%

Section: Application Of Phytase Using Different Feed Additivesmentioning

confidence: 99%

See 1 more Smart Citation

Effect of a mixed substrate on phytase production by Rhizopus oligosporus MTCC 556 using solid state fermentation and determination of dephytinization activities in food grains

Suresh

Radha

2015

Food Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…The metal ion requirement of Bacillus subtilis phytase has been studied and their removal from the medium by EDTA resulted in complete inactivation of the product while the enzymatic activities of fungal phytases are reported not to require metal ion [20]. Rhizopus oligosporus phytase activity was found to be unaVected or moderately stimulated by a range of metal ions [4]. Similarly, Aspergillus niger CFR 335 phytase was activated in presence of Ca 2+ in SmF at 0.01% (0.036 moles/L) level indicating Ca 2+ ion dependency of phytase, which might be acting as its cofactor.…”

Section: Evect Of Metal Salts On Aspergillus Niger Cfr 335 Phytase Acmentioning

confidence: 99%

Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations

Gunashree

Venkateswaran

2008

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

The present article deals with the studies on the effect of media ingredients, such as carbon, nitrogen, inorganic phosphates, surfactants, and metal salts, on phytase enzyme production by Aspergillus niger CFR 335 in submerged (SmF) and solid-state fermentations (SSF). The results obtained showed a 1.5-fold higher enzyme yield in the presence of sucrose in both SmF and SSF, while peptone was found to be a favorable nitrogen source for SmF. Sodium dihydrogen phosphate (NaH(2)PO(4)) favored 34% higher enzyme yield than the control, which was followed by 19% higher activity in potassium dihydrogen phosphate (KH(2)PO(4)) in SSF at 0.015% w/v. The addition of Tween-20 in SmF showed a maximum yield of 12.6 U/mL while, SDS suppressed the growth of the fungus. None of the surfactants favored the enzyme yield in SSF. Calcium chloride (CaCl(2)) was extensively efficient in stimulating more than 55% higher phytase production in SmF at 0.01% v/v. In SSF, none of the metal salts stimulated phytase production.

show abstract

“…According to HURRELL (2004), removing phytic acid facilitates ingredient digestion because it prevents the chelation of myoinositol hexaphosphate molecules with multivalent metal ions, especially zinc and calcium. Phytate also binds with proteins, amino acids, starch and enzymes such pepsin, trypsin and alpha amylase, compromising digestion and absorption (CASEY & WALSH, 2004). The low nutrient digestibility observed with DRB compared to DRB+Phy was also observed by SILVA et al (2005), when phytase supplementation improved the apparent ileal digestibility of amino acids from defatted rice bran and soybean meal in growing pigs.…”

Section: Resultsmentioning

confidence: 79%

Nutritional and energetic value of rice by-products, with or without phytase, for growing pigs

et al. 2015

View full text Add to dashboard Cite

show abstract

Identification and characterization of a phytase of potential commercial interest

Cited by 83 publications

References 30 publications

Effect of a mixed substrate on phytase production by Rhizopus oligosporus MTCC 556 using solid state fermentation and determination of dephytinization activities in food grains

Effect of a mixed substrate on phytase production by Rhizopus oligosporus MTCC 556 using solid state fermentation and determination of dephytinization activities in food grains

Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations

Nutritional and energetic value of rice by-products, with or without phytase, for growing pigs

Contact Info

Product

Resources

About