Identification and Characterization of a Juvenile Hormone (JH) Response Region in the JH Esterase Gene from the Spruce Budworm, Choristoneura fumiferana

Kethidi, Damodar R .; Perera, Srini C.; Zheng, Sichun; Feng, Qili; Krell, Peter J.; Retnakaran, Arthur; Palli, Subba Reddy

doi:10.1074/jbc.m311647200

Cited by 57 publications

(49 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…By contrast, because JHE is a critical JH-degradative enzyme, its substrate specificity and its functional role in the reduction of the JH titer have been proven (11,43). Furthermore, in a lepidopteran, Choristoneura fumiferana, JHE expression is directly regulated by both JH and 20E in dose-and time-dependent manners (10), and a 30-bp-long JH-responsive region within the JHE promoter has been identified (44). Taking into consideration these previous approaches, the manipulation of JHE gene expression for JH titer regulation will provide a forceful tool for the better understanding of the JH signaling pathway.…”

Section: Resultsmentioning

confidence: 99%

Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase

Tan

Tanaka

Tamura

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

165

109

View full text Add to dashboard Cite

Insect growth and development are intricately regulated by the titers of juvenile hormones (JHs) and ecdysteroids (and͞or their metabolites) in the insect hemolymph. Hydrolysis of the methyl ester of JH by a JH-specific esterase (JHE) is a key pathway for the degradation of JH. Here, we generate transgenic silkworm strains that overexpress JHE by using the binary GAL4͞UAS system. Overexpression of JHE from the embryonic stage resulted in larval-pupal metamorphosis after the third stadium, two stadia earlier than that observed in wild-type insects. This precocious metamorphosis suggests that JHs are not critical for normal development of embryo or larva before the second molt in Lepidoptera (moths and butterflies). Our transgenic approach allowed us to dissect the function of key physiological events that occur from embryogenesis. Until now, these types of studies were possible only in later larval stadia by using physical techniques such as allatectomy or the application of JH analogues. We believe that our system will allow further pioneering studies in insect physiology.Bombyx mori ͉ juvenile hormones ͉ transgenesis T wo major groups of nonpeptidic hormones, juvenile hormones (JHs) and ecdysteroids, play critical roles in the regulation of insect growth and development (1). The presence of JH during exposure to the ecdysteroids ensures a molt to a like stage, whereas the absence of JH during an ecdysteroid pulse allows metamorphosis. Concentrations of both hormones are regulated precisely by biosynthesis and degradation. Two definitive primary pathways for JH metabolism, hydrolysis of the methyl ester of JH by soluble esterases and hydration of the epoxide by microsomal epoxide hydrolases, have been described so far (2-5). In several insect orders, the key route of JH metabolism is by means of the hydrolysis of the methyl ester, and JH esterase (JHE) is considered the principal enzyme responsible for this function (2). Because of their importance in insect development, JHEs have been isolated and characterized from many insect orders such as Coleoptera (6), Diptera (7), and Lepidoptera (8-10), including the silkworm, Bombyx mori (11).As the primary degradative enzyme of JH, JHE has been used as an attractive tool for the study of JH action. Inhibition of JHE activity by a variety of 3-substituted 1,1,1-trifluoropropanone sulfides reduces the rate of JH degradation, thus delaying metamorphosis and resulting in giant larvae of Manduca sexta (12). By contrast, recombinant baculoviruses expressing JHE can reduce JH levels in Lepidoptera (13). However, difficulties in establishing stable and prompt in vivo expression of JHE in Lepidoptera have halted further studies.The mechanism of JH action in insects still remains a mystery despite a great deal of knowledge about its biological actions (5). Using Drosophila as a model for the study of JH action meets with difficulties because, in contrast to most insects, including those in Lepidoptera, Drosophila displays few morphogenetic actions that can be easily bioassayed ...

show abstract

Section: Resultsmentioning

confidence: 99%

Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase

Tan

Tanaka

Tamura

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

165

109

View full text Add to dashboard Cite

show abstract

“…Electrophoretic Mobility Shift Assay (EMSA)-To test the binding of the proteins to regulatory sequences, EMSA experiments were performed as described previously (26,27). The DNA oligonucleotides containing the consensus binding sites of Antp and POU-M2 were labeled using Cy3 at the 5Ј-end and annealed to produce double-stranded probes.…”

Section: Rna Extraction and Quantitative Rt-pcr (Qrt-pcr)mentioning

confidence: 99%

The Homeodomain Transcription Factors Antennapedia and POU-M2 Regulate the Transcription of the Steroidogenic Enzyme Gene Phantom in the Silkworm

Meng

Cheng

Peng

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background:The transcriptional regulation of steroidogenic enzymes in the silkworm remains poorly understood. Results: Antp and POU-M2 are expressed in the PG and regulate the transcription of Phantom. Conclusion: Antp and POU-M2 coordinate the transcription of Phantom via a protein interaction. Significance: Our study indicates new roles for homeodomain proteins in regulating insect ecdysteroidogenesis.

show abstract

“…At the molecular level, JH modifies or suppresses the 20E-triggered transcriptional cascade and downregulates caspase genes (Wu et al, 2006;Parthasarathy and Palli, 2007a;Parthasarathy and Palli, 2008). In addition, JH can directly affect gene expression independent of 20E (Kethidi et al, 2004;Li et al, 2007). Unfortunately, the molecular mechanism by which JH regulates gene expression remains unknown (Gilbert et al, 2000;Riddiford, 2008).…”

Section: Introductionmentioning

confidence: 99%

Juvenile hormone counteracts the bHLH-PAS transcription factors MET and GCE to prevent caspase-dependent programmed cell death inDrosophila

et al. 2009

View full text Add to dashboard Cite

Juvenile hormone (JH) regulates many developmental and physiological events in insects, but its molecular mechanism remains conjectural. Here we report that genetic ablation of the corpus allatum cells of the Drosophila ring gland (the JH source) resulted in JH deficiency, pupal lethality and precocious and enhanced programmed cell death (PCD) of the larval fat body. In the fat body of the JH-deficient animals, Dronc and Drice, two caspase genes that are crucial for PCD induced by the molting hormone 20-hydroxyecdysone (20E), were significantly upregulated. These results demonstrated that JH antagonizes 20E-induced PCD by restricting the mRNA levels of Dronc and Drice. The antagonizing effect of JH on 20E-induced PCD in the fat body was further confirmed in the JH-deficient animals by 20E treatment and RNA interference of the 20E receptor EcR. Moreover, MET and GCE, the bHLH-PAS transcription factors involved in JH action, were shown to induce PCD by upregulating Dronc and Drice. In the Met-and gce-deficient animals, Dronc and Drice were downregulated, whereas in the Met-overexpression fat body, Dronc and Drice were significantly upregulated leading to precocious and enhanced PCD, and this upregulation could be suppressed by application of the JH agonist methoprene. For the first time, we demonstrate that JH counteracts MET and GCE to prevent caspase-dependent PCD in controlling fat body remodeling and larval-pupal metamorphosis in Drosophila.

show abstract

Identification and Characterization of a Juvenile Hormone (JH) Response Region in the JH Esterase Gene from the Spruce Budworm, Choristoneura fumiferana

Cited by 57 publications

References 37 publications

Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase

Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase

The Homeodomain Transcription Factors Antennapedia and POU-M2 Regulate the Transcription of the Steroidogenic Enzyme Gene Phantom in the Silkworm

Juvenile hormone counteracts the bHLH-PAS transcription factors MET and GCE to prevent caspase-dependent programmed cell death inDrosophila

Contact Info

Product

Resources

About