The Homeodomain Transcription Factors Antennapedia and POU-M2 Regulate the Transcription of the Steroidogenic Enzyme Gene Phantom in the Silkworm

Meng, Meng; Cheng, Daojun; Peng, Jian; Qian, Wenliang; Li, Jiarui; Dai, Dandan; Zhang, Tianlei; Xia, Qingyou

doi:10.1074/jbc.m115.651810

Cited by 24 publications

(31 citation statements)

References 72 publications

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“…Such gene expressions are induced and/or maintained by a number of TFs, hereinafter referred to as "ecdysteroidogenic TFs". Some but not all ecdysteroidogenic TFs directly bind to the promoters of ecdysteroidogenic genes, confirmed by an electrophoresis mobility shift assay or chromatin immunoprecipitation assay (Xiang et al 2010;Deng & Kerppola 2013;Danielsen et al 2014;Meng et al 2015). The known validated and putative ecdysteroidogenic TFs required in the PG are listed in Table 1.…”

Section: Transcription Factors Are Required For Expression Of Ecdystementioning

confidence: 85%

“…; Meng et al . ). The known validated and putative ecdysteroidogenic TFs required in the PG are listed in Table .…”

Section: Transcription Factors Are Required For Expression Of Ecdystementioning

confidence: 97%

“…) and B. mori (referred as POU‐M2 ) (Meng et al . ). Therefore, the β Ftz‐f1/SF‐1 and Vvl/POU3F2 families emphasize the conserved regulatory mechanisms of steroidogenesis between insects and vertebrates.…”

Section: The Evolutionarily Conserved Function Of Steroidogenic Tfsmentioning

confidence: 97%

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Transcriptional regulation of insect steroid hormone biosynthesis and its role in controlling timing of molting and metamorphosis

Niwa

2015

Dev Growth Differ

116

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The developmental transition from juvenile to adult is often accompanied by many systemic changes in morphology, metabolism, and reproduction. Curiously, both mammalian puberty and insect metamorphosis are triggered by a pulse of steroid hormones, which can harmonize gene expression profiles in the body and thus orchestrate drastic biological changes. However, understanding of how the timing of steroid hormone biosynthesis is regulated at the molecular level is poor. The principal insect steroid hormone, ecdysteroid, is biosynthesized from dietary cholesterol in the specialized endocrine organ called the prothoracic gland. The periodic pulses of ecdysteroid titers determine the timing of molting and metamorphosis. To date, at least nine families of ecdysteroidogenic enzyme genes have been identified. Expression levels of these genes correlate well with ecdysteroid titers, indicating that the transcriptional regulatory network plays a critical role in regulating the ecdysteroid biosynthesis pathway. In this article, we summarize the transcriptional regulation of ecdysteroid biosynthesis. We first describe the development of prothoracic gland cells during Drosophila embryogenesis, and then provide an overview of the transcription factors that act in ecdysteroid biosynthesis and signaling. We also discuss the external signaling pathways that target these transcriptional regulators. Furthermore, we describe conserved and/or diverse aspects of steroid hormone biosynthesis in insect species as well as vertebrates.

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Section: Transcription Factors Are Required For Expression Of Ecdystementioning

confidence: 85%

“…; Meng et al . ). The known validated and putative ecdysteroidogenic TFs required in the PG are listed in Table .…”

Section: Transcription Factors Are Required For Expression Of Ecdystementioning

confidence: 97%

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Transcriptional regulation of insect steroid hormone biosynthesis and its role in controlling timing of molting and metamorphosis

Niwa

2015

Dev Growth Differ

116

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“…Deng and colleagues reported that BmBrC‐Z4 is an up‐stream regulator of BmPOUM2 but that the two do not directly interact (Deng et al ., ), and that BmPOUM2 plays a role in regulating the wing disc culticle protein 4 (WCP4) gene expression (Deng et al ., ). BmPOUM2 participates in regulating steroidogenic enzyme genes to control ecdysteroidogenesis (Meng et al ., ). In this study, we confirmed that a key member of the Hox family of transcription factors in the silkworm, BmPOUM2, participates in the regulation of BmVg expression.…”

Section: Discussionmentioning

confidence: 97%

“…() showed that BmPOUM2 takes part in regulating the expression of the genes encoding wing disc cuticle proteins during silkworm metamorphosis. Furthermore, BmPOUM2 was found to participate in regulating steroidogenic enzyme genes to control ecdysteroidogenesis (Meng et al ., ).…”

Section: Introductionmentioning

confidence: 99%

The POU homeodomain transcription factor POUM2 and broad complex isoform 2 transcription factor induced by 20‐hydroxyecdysone collaboratively regulate vitellogenin gene expression and egg formation in the silkworm Bombyx mori

Lin

Liu

Yang

et al. 2017

Insect Molecular Biology

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Vitellogenin (Vg) is a source of nutrition for embryo development. Our previous study showed that the silkworm (Bombyx mori) transcription factor broad complex isoform 2 (BmBrC-Z2) regulates gene expression of the Vg gene (BmVg) by induction with 20-hydroxyecdysone (20E). However, the mechanism by which 20E regulates BmVg expression was not clarified. In this study, cell transfection experiments showed that the BmVg promoter containing the POU homeodomain transcription factor POUM2 (POUM2) and BrC-Z2 cis-response elements (CREs) showed a more significant response to 20E than that harbouring only the BrC-Z2 or POUM2 CRE. An electrophoretic mobility shift assay and chromatin immunoprecipitation assay showed that BmPOUM2 could bind to the POUM2 CRE of the BmVg promoter. Over-expression of BmPOUM2 and BmBrC-Z2 in B. mori embryo-derived cell line (BmE) could enhance the activity of the BmVg promoter carrying both the POUM2 and BrC-Z2 CREs following 20E induction. Quantitative PCR and immunofluorescence histochemistry showed that the expression pattern and tissue localization of BmPOUM2 correspond to those of BmVg. Glutathione S-transferase pull-down and co-immunoprecipitation assays confirmed that BmPOUM2 interacts only with BmBrC-Z2 to regulate BmVg expression. Down-regulation of BmPOUM2 in female silkworm by RNA interference significantly reduced BmVg expression, leading to abnormal egg formation. In summary, these results indicate that BmPOUM2 binds only to BmBrC-Z2 to collaboratively regulate BmVg expression by 20E induction to control vitellogenesis and egg formation in the silkworm. Moreover, these findings suggest that homeodomain protein POUM2 plays a novel role in regulating insect vitellogenesis.

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Transcription factor E74A affects the ecdysone titer by regulating the expression of the EO gene in the silkworm, Bomby mori

Sun

Wang

Zhang

2017

Biochimica et Biophysica Acta (BBA) - General Subjects

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The Homeodomain Transcription Factors Antennapedia and POU-M2 Regulate the Transcription of the Steroidogenic Enzyme Gene Phantom in the Silkworm

Cited by 24 publications

References 72 publications

Transcriptional regulation of insect steroid hormone biosynthesis and its role in controlling timing of molting and metamorphosis

Transcriptional regulation of insect steroid hormone biosynthesis and its role in controlling timing of molting and metamorphosis

The POU homeodomain transcription factor POUM2 and broad complex isoform 2 transcription factor induced by 20‐hydroxyecdysone collaboratively regulate vitellogenin gene expression and egg formation in the silkworm Bombyx mori

Transcription factor E74A affects the ecdysone titer by regulating the expression of the EO gene in the silkworm, Bomby mori

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