Identification and characterization of antioxidative peptides derived from simulated in vitro gastrointestinal digestion of walnut meal proteins

Feng, Li; Peng, Fei; Wang, Xiaojing; Li, Mei; Lei, Hongjie; Xu, Huaide

doi:10.1016/j.foodres.2018.08.068

Cited by 97 publications

(62 citation statements)

References 34 publications

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“…Similar results have been shown in previous studies, such as SPH-I (Mw < 3 kDa) from the alcalase-hydrolyzed soybean hydrolysate (SPH) which decreased the death rate of injured Caco-2 cells induced by oxidative stress, increased CAT activity, and decreased MDA content [48]. Feng et al [49] reported that two peptides (TY and SGGY) from defatted walnut meal protein hydrolysates (DEMPH) had the protective effect against oxidative damage to SH-SY5Y cells, therefore SGGY could significantly increase cell viability, and improve GSH content at a concentration of 1.0 mg/mL (p < 0.01). Therefore, all the results suggested that mung bean protein hydrolysates exerted a protective effect on the injured NCTC-1469 cells through the inhibition of lipid peroxides and increasing antioxidant enzyme activity.…”

Section: Effect Of Mbphs-i On the Level Of Ros On Cellssupporting

confidence: 86%

Mung Bean Protein Hydrolysates Protect Mouse Liver Cell Line Nctc-1469 Cell from Hydrogen Peroxide-Induced Cell Injury

Xie

et al. 2019

Foods

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Mung bean is nutritious and rich in protein (19.5%-33.1%). However, there are few studies on mung bean protein active peptides so the mung bean protein hydrolysates (MBPHs) were investigated for evaluating their ability to clear intracellular reactive oxygen species (ROS) and regulating the ability of antioxidant enzymes on NCTC-1469 cells. Results showed that MBPHs, MBPHs-I (molecular weight < 3 kDa), MBPHs-II (molecular weight between 3 and 10 kDa), and MBPHs-III (molecular weight > 10 kDa) could all improve the survival rate of cells compared with the model group. MBPHs, MBPHs-I, and MBPHs-II could significantly decrease the content of lactate dehydrogenase (LDH) and reduce the generation of malonaldehyde (MDA) at a concentration of 0.4 mg/mL. Regarding the intracellular ROS, the result showed that MBPHs-I significantly reduced the production of ROS (from 58.3% to 26.6%) and had a dose-dependent relationship. In addition, the amino acid analysis showed that MBPHs-I had a balanced amino acid composition. MBPHs-I is rich in lysine but was deficient in cereals. Therefore, the hydrophobic and aromatic amino acids in MBPHs-I were high, which could improve its antioxidant activity. According to the results, MBPHs-I was the best and most potent natural antioxidant and it can contribute to drug development and medical application.

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Section: Effect Of Mbphs-i On the Level Of Ros On Cellssupporting

confidence: 86%

Mung Bean Protein Hydrolysates Protect Mouse Liver Cell Line Nctc-1469 Cell from Hydrogen Peroxide-Induced Cell Injury

Xie

et al. 2019

Foods

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“…Feng et al isolated six walnut peptides from the simulated digestion products of walnut meal protein, and characterized their antioxidant activity and molecular structure, respectively. According to the structure-activity relationship of the six peptides, they proposed that the amino acid sequence and composition of the peptides play an important role in the antioxidant activity [21]. The report from Chen et al [43] is slightly different from that of Feng et al [21].…”

Section: Antioxidant Peptidementioning

confidence: 97%

“…The enzymes used for the preparation of WBP must follow the same requirements as other industrial enzymes, which requires not only a wide range of sources, but also a relatively low price. According to the literature, the commonly used walnut proteolytic enzymes are pepsin [19][20][21], trypsin [22,23], alkaline protease [24], papain [25], neutral protease [26], composite protease (pancreatin) [21], etc. As special proteins with physiological activity, enzymes have different activation centers and hydrolysis specificity.…”

Section: Enzymatic Preparationmentioning

confidence: 99%

“…Mass spectrometry and other hyphenated techniques can complement Edman sequencing. At present, they are widely used in the analysis of amino acid sequence of walnut peptide, such as electrospray ionization mass spectrometry (ESI-MS) [24,36], matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) [40,41], quadrupole time-of-flight mass spectrometry (Q-TOF-MS) [21,42], determination of clenbuterol by liquid chromatography-tandem mass spectrometry (LC-MS/MS) [19] and so on. In recent years, de novo sequencing method has shown significant advantages in the structural identification of walnut peptides [24,36,43,44].…”

Section: Identification Of Walnut Active Peptidementioning

confidence: 99%

“…According to the structure-activity relationship of the six peptides, they proposed that the amino acid sequence and composition of the peptides play an important role in the antioxidant activity [21]. The report from Chen et al [43] is slightly different from that of Feng et al [21]. Chen et al identified seventy-seven peptides from the trypsin hydrolysate of defatted walnut residue, and isolated WSREEQEREE and ADIYTEEAGR, two walnut peptides with strong protective effect on PC12 cells damaged by H 2 O 2 .…”

Section: Antioxidant Peptidementioning

confidence: 99%

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Bioactive Peptides from Walnut Residue Protein

Guo

Chi

et al. 2020

Molecules

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Walnut residue is a kind of high-quality plant protein resource. The bioactive peptide prepared from walnut residue has excellent health care functions such as antioxidation and antihypertensive activity, but at present, walnut residue is often regarded as waste or low value feed, fertilizer and other materials. The uneconomical use of walnut residue has hindered the development of the walnut industry to some extent. Effective utilization of walnut residue protein to develop bioactive peptides and other products is of great significance to realize the comprehensive utilization of walnut residue, improve the added value of by-products, and change the current low utilization rate of walnut residue. In this paper, the preparation, purification and structure identification of walnut protein bioactive peptides are reviewed, and different functional walnut active peptides (WBPs) are introduced. The potential effects of these bioactivities on human health and their different uses in food, medicine and other industries are discussed. The purpose is to provide reference information for the effective utilization of walnut residue resources and the development of walnut industry.

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Purification and Identification of Pine Nut (Pinus yunnanensis Franch.) Protein Hydrolysate and Its Antioxidant Activity in Vitro and in Vivo

Liu

Shi

Liu

et al. 2020

Chemistry & Biodiversity

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In this study, the pine nut (Pinus yunnanensis Franch.) protein was hydrolyzed by alkaline protease and trypsin to prepare pine nut protein hydrolysate (PNPH). The chemical, intracellular and in vivo antioxidant capacity of PNPH were evaluated. PNPH owned the ability of scavenging free radicals, and it could protect the HepG2 cells from oxidative damage by preserving cell viability. Moreover, PNPH could reduce the malondialdehyde (MDA) content and improved the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in serum, heart and liver of aging mice induced by D-galactose. Further, the PNPH was stepwise purified and identified, and 15 peptides were identified from purified fraction in PNPH. The three-dimension structures of identified peptides were predicted. Among all identified peptides, peptide 3, 7, 8 and 11 were presumed to possess good antioxidant activity. Overall, PNPH and purified peptides isolated from PNPH have potential application prospects in the field of natural antioxidants and anti-aging functional foods.

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Identification and characterization of antioxidative peptides derived from simulated in vitro gastrointestinal digestion of walnut meal proteins

Cited by 97 publications

References 34 publications

Mung Bean Protein Hydrolysates Protect Mouse Liver Cell Line Nctc-1469 Cell from Hydrogen Peroxide-Induced Cell Injury

Mung Bean Protein Hydrolysates Protect Mouse Liver Cell Line Nctc-1469 Cell from Hydrogen Peroxide-Induced Cell Injury

Bioactive Peptides from Walnut Residue Protein

Purification and Identification of Pine Nut (Pinus yunnanensis Franch.) Protein Hydrolysate and Its Antioxidant Activity in Vitro and in Vivo

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