Identification and characterization of Aspergillus species of fruit rot fungi using microscopy, FT-IR, Raman and UV–Vis spectroscopy

Saif, F. A.; Yaseen, S. A.; Alameen, A. S.; Mane, Shrikant B; Undre, P. B.

doi:10.1016/j.saa.2020.119010

Cited by 54 publications

(21 citation statements)

References 44 publications

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“…Confocal Raman micro-spectroscopy (CRM) is highly molecular specific, reliable, easy to use, and reproducible, requires less sample and less pretreatment of sample, and can be used to study solids, liquids, and gases [14] . CRM gives wide information about the chemical composition of microorganisms such as fungi, bacteria, and algae at the single-cell level [15] . Recently, Raman and FTIR spectroscopy has been also applied for the characterization of the various EOs [16] , [17] .…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a novel green tea essential oil nanoemulsion and its antifungal mechanism of action against Magnaporthae oryzae

Perumal

Liu

et al. 2021

Ultrasonics Sonochemistry

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a novel green tea essential oil nanoemulsion and its antifungal mechanism of action against Magnaporthae oryzae

Perumal

Liu

et al. 2021

Ultrasonics Sonochemistry

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“…However, the definite assignment of the two melanin bands is not entirely agreed upon. Some authors ascribe both bands to the C-C stretching bonds within the aromatic rings, whereas others mark a contribution of aromatic C-N and C-H deformation bonds in the 1400 cm -1 region [44][45][46][47][48][49][50]. According to Culka et al [43], this uncertainty is probably due to the vibrations of the constituent monomers, resulting in many overlapping Raman bands.…”

Section: Pigment Characterization By Raman Spectroscopymentioning

confidence: 99%

The Ground-Based BIOMEX Experiment Verification Tests for Life Detection on Mars

Pacelli

Cassaro

Catanzaro

et al. 2021

Life

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The success of an astrobiological search for life campaign on Mars, or other planetary bodies in the Solar System, relies on the detectability of past or present microbial life traces, namely, biosignatures. Spectroscopic methods require little or no sample preparation, can be repeated almost endlessly, and can be performed in contact or even remotely. Such methods are therefore ideally suited to use for the detection of biosignatures, which can be confirmed with supporting instrumentation. Here, we discuss the use of Raman and Fourier Transform Infrared (FT-IR) spectroscopies for the detection and characterization of biosignatures from colonies of the fungus Cryomyces antarcticus, grown on Martian analogues and exposed to increasing doses of UV irradiation under dried conditions. The results report significant UV-induced DNA damage, but the non-exceeding of thresholds for allowing DNA amplification and detection, while the spectral properties of the fungal melanin remained unaltered, and pigment detection and identification was achieved via complementary analytical techniques. Finally, this work found that fungal cell wall compounds, likely chitin, were not degraded, and were still detectable even after high UV irradiation doses. The implications for the preservation and detection of biosignatures in extraterrestrial environments are discussed.

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“…Although various authors who analyzed a fungal biomass associated the peaks between 1465 and 1450 cm −1 with the vibrations of the CH groups in lipids (Bhat, 2013;Lecellier et al, 2014;Haneef et al, 2017;Kosa et al, 2018), it is worth noting that the interval between 1500 and 1300 cm −1 is often termed as the "mixed" region. Besides lipids, vibrations of functional groups bound in polysaccharides, proteins and triterpene compounds may be detected in the considered interval (Meenu and Xu, 2019;Saif et al, 2021). The majority of absorption at 1430 cm −1 was probably caused by C-O-H bending in polysaccharides (Song et al, 2015;Meenu and Xu, 2019), but some contributions from the COO − group in carboxylic acids cannot be ruled out (Max and Chapados, 2004;Stuart, 2004).…”

Section: Fourier-transform Infrared Spectra Of Chlorella Sp and Aspergillus Niger Biomassmentioning

confidence: 99%

Experimental Treatment of Hazardous Ash Waste by Microbial Consortium Aspergillus niger and Chlorella sp.: Decrease of the Ni Content and Identification of Adsorption Sites by Fourier-Transform Infrared Spectroscopy

et al. 2021

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Despite the negative impact on the environment, incineration is one of the most commonly used methods for dealing with waste. Besides emissions, the production of ash, which usually shows several negative properties, such as a higher content of hazardous elements or strongly alkaline pH, is problematic from an environmental viewpoint as well. The subject of our paper was the assessment of biosorption of Ni from ash material by a microbial consortium of Chlorella sp. and Aspergillus niger. The solid substrate represented a fraction of particles of size <0.63 mm with a Ni content of 417 mg kg–1. We used a biomass consisting of two different organisms as the sorbent: a non-living algae culture of Chlorella sp. (an autotrophic organism) and the microscopic filamentous fungus A. niger (a heterotrophic organism) in the form of pellets. The experiments were conducted under static conditions as well as with the use of shaker (170 rpm) with different modifications: solid substrate, Chlorella sp. and pellets of A. niger; solid substrate and pellets of A. niger. The humidity-temperature conditions were also changed. Sorption took place under dry and also wet conditions (with distilled water in a volume of 30–50 ml), partially under laboratory conditions at a temperature of 25°C as well as in the exterior. The determination of the Ni content was done using inductively coupled plasma optical emission spectrometry (ICP-OES). The removal of Ni ranged from 13.61% efficiency (Chlorella sp., A. niger with the addition of 30 ml of distilled water, outdoors under static conditions after 48 h of the experiment) to 46.28% (Chlorella sp., A. niger with the addition of 30 ml of distilled water, on a shaker under laboratory conditions after 48 h of the experiment). For the purpose of analyzing the representation of functional groups in the microbial biomass and studying their interaction with the ash material, we used Fourier-transform infrared (FTIR) spectroscopy. We observed that the amount of Ni adsorbed positively correlates with absorbance in the spectral bands where we detect the vibrations of several organic functional groups. These groups include hydroxyl, aliphatic, carbonyl, carboxyl and amide structural units. The observed correlations indicate that, aside from polar and negatively charged groups, aliphatic or aromatic structures may also be involved in sorption processes due to electrostatic attraction. The correlation between absorbance and the Ni content reached a maximum in amide II band (r = 0.9; P < 0.001), where vibrations of the C=O, C–N, and N–H groups are detected. The presented results suggest that the simultaneous use of both microorganisms in biosorption represents an effective method for reducing Ni content in a solid substrate, which may be useful as a partial process for waste disposal.

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Identification and characterization of Aspergillus species of fruit rot fungi using microscopy, FT-IR, Raman and UV–Vis spectroscopy

Cited by 54 publications

References 44 publications

Preparation and characterization of a novel green tea essential oil nanoemulsion and its antifungal mechanism of action against Magnaporthae oryzae

Preparation and characterization of a novel green tea essential oil nanoemulsion and its antifungal mechanism of action against Magnaporthae oryzae

The Ground-Based BIOMEX Experiment Verification Tests for Life Detection on Mars

Experimental Treatment of Hazardous Ash Waste by Microbial Consortium Aspergillus niger and Chlorella sp.: Decrease of the Ni Content and Identification of Adsorption Sites by Fourier-Transform Infrared Spectroscopy

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