Identification and Characterization of Clinical Isolates of Members of the
            <i>Staphylococcus sciuri</i>
            Group

Stepanović, Srdjan; Dakić, Ivana; Morrison, Donald A.; Hauschild, Tomasz; Ježek, Petr; Petřáš, Petr; Martel, An; Vuković, Dragana; Shittu, Adebayo; Devriese, Luc

doi:10.1128/jcm.43.2.956-958.2005

Cited by 61 publications

(52 citation statements)

References 26 publications

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“…Since animals and the environment are considered the main reservoirs of S. sciuri, the result of particular interest is the presence of relatively high numbers of isolates with the mecA gene among animal (26.5%) and environmental (37.8%) S. sciuri strains. S. lentus and S. vitulinus are less frequently isolated from humans than S. sciuri (29). In this study the mecA gene was not detected in any of the isolates of these two species tested, irrespective of their origin.…”

Section: Discussioncontrasting

confidence: 54%

“…The isolates were stored at Ϫ80°C until they were analyzed. Some of the isolates have been reported previously but were not investigated for their oxacillin resistance by the methods presented in this study (7,29,33).…”

Section: Methodsmentioning

confidence: 99%

“…They constitute between 0.79% and 4.3% of the total number of CoNS isolated from human clinical samples (12,31). Although they are rarely isolated from humans, 21.4% of the S. sciuri group isolates were found to be clinically significant (29). These bacteria have been associated with serious human infections, such as endocarditis (14), peritonitis (37), septic shock (15), urinary tract infections (31), endophthalmitis (1), pelvic inflammatory disease (32), and, most frequently, wound infections (28,30).…”

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confidence: 99%

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Evaluation of Phenotypic and Molecular Methods for Detection of Oxacillin Resistance in Members of the Staphylococcus sciuri Group

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In this paper we report on an experimental evaluation of phenotypic and molecular methods as means for the detection of oxacillin resistance in members of the Staphylococcus sciuri group. A total of 109 S. sciuri group member isolates (92 S. sciuri isolates, 9 S. lentus isolates, and 8 S. vitulinus isolates) were tested by the disk diffusion method, the agar dilution method, the oxacillin salt-agar screening method, slide latex agglutination for PBP 2a, and PCR assay for mecA as the reference method. The mecA gene was detected in 29 S. sciuri isolates, and the true-positive and true-negative results of the other tests were defined on the basis of the presence or the absence of the mecA gene. For the different methods evaluated, the sensitivities and specificities were as follows: for the disk diffusion test with a 1-g oxacillin disk, 100% and 55.9%, respectively; for the disk diffusion test with a 30-g cefoxitin disk, 93.5% and 100%, respectively; for the agar dilution method, 100% and 50%, respectively; for the oxacillin salt-agar screen test (with 6 g of oxacillin per ml and 4% NaCl) 100% and 100%, respectively; and for the slide latex agglutination test for PBP 2a, 100% and 100%, respectively. The disk diffusion test with various ␤-lactam antibiotics was performed to evaluate their use for the prediction of oxacillin resistance. The results indicate that meropenem, cefazolin, cefamandole, cefuroxime, cefotetan, cefoperazone, cefotaxime, ceftriaxone, moxalactam, cefaclor, and cefprozil may be used as surrogate markers of oxacillin resistance, although further studies of their use for the detection of oxacillin resistance are required.Staphylococcus sciuri, Staphylococcus lentus, and Staphylococcus vitulinus are novobiocin-resistant, oxidase-positive, coagulase-negative staphylococci (CoNS) which compose the Staphylococcus sciuri group. The rate of isolation of these bacteria from humans ranges from 0.042% from the urinary tract (31) to 0.087% from the female genital tract (32). They constitute between 0.79% and 4.3% of the total number of CoNS isolated from human clinical samples (12, 31). Although they are rarely isolated from humans, 21.4% of the S. sciuri group isolates were found to be clinically significant (29). These bacteria have been associated with serious human infections, such as endocarditis (14), peritonitis (37), septic shock (15), urinary tract infections (31), endophthalmitis (1), pelvic inflammatory disease (32), and, most frequently, wound infections (28, 30). The sources of S. sciuri group bacteria for humans are animals (13, 16, 33); food of animal origin (10, 24); and the environment, such as soil, sand, and water (16, 23), as well as the hospital environment (7).Oxacillin-resistant staphylococci are recognized as important nosocomial and, recently, community pathogens. The major mechanism of oxacillin resistance in staphylococci is mediated by the production of PBP 2a, which is encoded by the mecA gene (3). S. sciuri attracted special attention after it was suggested that the mecA gene o...

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Section: Discussioncontrasting

confidence: 54%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Evaluation of Phenotypic and Molecular Methods for Detection of Oxacillin Resistance in Members of the Staphylococcus sciuri Group

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“…The clinical isolates consisted of five S. aureus strains, seven S. haemolyticus strains, three S. epidermidis strains, four S. sciuri strains, one S. vitulinus strain, one S. caprae strain, one S. cohnii subsp. cohnii strain, and one S. hominis strain that were previously identified by use of the BD Phoenix system or reference biochemical tests (3,20).Chromosomal DNA isolation. Chromosomal DNAs from all staphylococcal strains were obtained from overnight cultures grown in 3 ml of brain heart infusion broth.…”

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Identification of Staphylococcus spp. by PCR-Restriction Fragment Length Polymorphism Analysis of dnaJ Gene

Hauschild

Stepanović

2008

J Clin Microbiol

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A PCR-restriction fragment length polymorphism (RFLP) analysis method that analyzes a part of the dnaJ gene was designed for the rapid and accurate identification of Staphylococcus spp. XapI or Bsp143I digestion of the PCR-generated products rendered distinctive RFLP patterns that allowed 41 reference species and subspecies to be identified with a high degree of specificity. The novel method was validated by the identification of 23 clinical staphylococcal strains, and the results were compared with those obtained by other genotypic identification methods. A 100% concordance of the results was shown. Therefore, PCR-RFLP analysis of the dnaJ gene is proposed as a reliable and reproducible method for the identification of Staphylococcus spp.Staphylococci are widely distributed in various environments. Natural populations are associated with the skin, skin glands, and mucous membranes of humans and many animals alike. They are sometimes found in the intestinal, genitourinary, and upper respiratory tracts of these hosts. They have also been isolated from animal products and other sources, such as soil, sand, seawater, fresh water, dust, and air (9). So far, 40 species of the genus Staphylococcus have been identified (excluding Staphylococcus pulvereri as a separate species), 10 of which contain subdivisions with subspecies designations.Although Staphylococcus aureus is clinically the most significant Staphylococcus species, as it causes many types of infections, other coagulase-negative staphylococci (CoNS) are increasingly becoming recognized as etiologic agents of medical device-related infections in humans, as well as different types of infections in farm and pet animals. Consequently, it is becoming increasingly important to accurately identify these isolates to the species level in order to define the clinical significance of the bacteria in question, to carry out proper epidemiologic observations, and to manage CoNS-infected patients with relapses. A variety of manual and automated methods based on phenotypic characteristics have been developed for the identification of staphylococci, including conventional identification methods and several methods that use commercial kits. Unfortunately, the overall accuracies of these systems are low and range from 50 to 70% (6,8,15,16). Moreover, conventional reference methods are too laborious and timeconsuming to be used in clinical laboratories. Several problems associated with the systems mentioned above result from the variability in the expression of metabolic activities and/or the morphological features of some staphylococcal species (4); thus, if the strain has atypical characteristics, it may be difficult, if not impossible, to precisely assign the strains to the species level. Furthermore, commercial systems may offer two or more suggestions as to the species identification with comparable levels of safety. Due to the limited number of stable features that can be used for species discrimination, many taxa remain difficult to distinguish from one another and are m...

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“…The 48 S. sciuri group strains we analyzed were reported previously but not investigated for characteristics presented in this study. Twenty-eight strains were recovered from various human clinical samples (11), while the remaining 20 strains were recovered from an inanimate hospital environment (6). All strains were identified by conventional methods, and the identity was confirmed by PCR amplification of the 16S to 23S rRNA intergenic spacer region.…”

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Survey of Genes Encoding Staphylococcal Enterotoxins, Toxic Shock Syndrome Toxin 1, and Exfoliative Toxins in Members of the Staphylococcus sciuri Group

et al. 2005

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Identification and Characterization of Clinical Isolates of Members of the Staphylococcus sciuri Group

Cited by 61 publications

References 26 publications

Evaluation of Phenotypic and Molecular Methods for Detection of Oxacillin Resistance in Members of the Staphylococcus sciuri Group

Evaluation of Phenotypic and Molecular Methods for Detection of Oxacillin Resistance in Members of the Staphylococcus sciuri Group

Identification of Staphylococcus spp. by PCR-Restriction Fragment Length Polymorphism Analysis of dnaJ Gene

Survey of Genes Encoding Staphylococcal Enterotoxins, Toxic Shock Syndrome Toxin 1, and Exfoliative Toxins in Members of the Staphylococcus sciuri Group

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