Identification and characterization of Colletotrichum destructivum causing anthracnose on sunflower

Sun, Hui; Tian, Jiamei; Steinkellner, Siegrid; Liang, Yue

doi:10.1007/s00203-020-01861-8

Cited by 5 publications

(1 citation statement)

References 33 publications

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“…The growth of fungal colony was measured at 3-day post inoculation (dpi) for S. Sclerotinia and 4 dpi for B. cinerea with three inoculation replicates of each pathogen; the growth assays were independently repeated three times. The inhibition rate of ε-PL on the mycelial growth of S. sclerotiorum and B. cinerea was calculated by the following formula [31]: antifungal rate (%) = [(control − treated)/control] × 100%. The effective medium concentration (EC50) values of the agent on S. sclerotiorum and B. cinerea were calculated.…”

Section: Antifungal Activity Of ε-Pl In Vitromentioning

confidence: 99%

ε-poly-L-lysine Affects the Vegetative Growth, Pathogenicity and Expression Regulation of Necrotrophic Pathogen Sclerotinia sclerotiorum and Botrytis cinerea

Zhou

Huang

et al. 2021

JoF

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Microbial secondary metabolites produced by Streptomyces are applied to control plant diseases. The metabolite, ε-poly-L-lysine (ε-PL), is a non-toxic food preservative, but the potential application of this compound as a microbial fungicide in agriculture is rarely reported. In this study, the effect and mode of action of ε-PL on two necrotrophic pathogenic fungi, Sclerotinia sclerotiorum and Botrytis cinerea, were investigated. The results showed that ε-PL effectively inhibited the mycelial growth of S. sclerotiorum and B. cinerea with EC50 values of 283 μg/mL and 281 μg/mL, respectively. In addition, ε-PL at the dose of 150 and 300 μg/mL reduced S. sclerotiorum sclerotia formation. The results of the RNA-seq and RT-qPCR validation indicated that ε-PL significantly regulated the gene expression of critical differential expressed genes (DEGs) involved in fungal growth, metabolism, pathogenicity, and induced an increase in the expression of the fungal stress responses and the detoxification genes. These results provided new insights for understanding the modes of action of ε-PL on S. sclerotiorum and B. cinerea and improved the sustainable management of these plant diseases.

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Section: Antifungal Activity Of ε-Pl In Vitromentioning

confidence: 99%

ε-poly-L-lysine Affects the Vegetative Growth, Pathogenicity and Expression Regulation of Necrotrophic Pathogen Sclerotinia sclerotiorum and Botrytis cinerea

Zhou

Huang

et al. 2021

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Isolation and identification of Colletotrichum truncatum causing anthracnose disease of the Chinese medicinal herb Cynanchum paniculatum

Wang

et al. 2022

Canadian Journal of Plant Pathology

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Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Phoma macdonaldii, the Causal Agent of Sunflower Black Stem

Sun

Yang

et al. 2022

Plant Disease

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Phoma macdonaldii, the causal agent of sunflower black stem, severely affects sunflower yield and quality. A rapid and sensitive detection method is necessary for diagnosis of this disease. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of the pathogen from diseased sunflower tissues. The LAMP primers were designed targeting the rDNA region of the fungus. The reaction condition was optimized to 60°C water baths for 45 min. The detection limit of the LAMP assay was 100 fg DNA or 10 conidia/g seeds. The LAMP assay was validated by detecting P. macdonaldii from infected sunflower tissue samples including leaves, stems and seeds, and applied to seed samples randomly collected from sunflower fields. This LAMP assay will be useful to estimate disease prevalence and implement sustainable management of sunflower black stem.

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Identification and characterization of Colletotrichum destructivum causing anthracnose on sunflower

Cited by 5 publications

References 33 publications

ε-poly-L-lysine Affects the Vegetative Growth, Pathogenicity and Expression Regulation of Necrotrophic Pathogen Sclerotinia sclerotiorum and Botrytis cinerea

ε-poly-L-lysine Affects the Vegetative Growth, Pathogenicity and Expression Regulation of Necrotrophic Pathogen Sclerotinia sclerotiorum and Botrytis cinerea

Isolation and identification of Colletotrichum truncatum causing anthracnose disease of the Chinese medicinal herb Cynanchum paniculatum

Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Phoma macdonaldii, the Causal Agent of Sunflower Black Stem

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