Identification and characterization of Fusarium spp. associated with root rots of field pea in North Dakota

Chittem, Kishore; Mathew, Febina M.; Gregoire, Matthew; Lamppa, Robin S.; Chang, Y. W.; Markell, Samuel G.; Bradley, Carl A.; Barasubiye, Tharcisse; Goswami, Rubella S.

doi:10.1007/s10658-015-0714-8

Cited by 63 publications

(60 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Total RNA was isolated using an RNeasy®Mini Kit with an on-column DNase digestion (Qiagen, Montréal, QC, Canada), followed by an additional DNase digest with RNase-Free DNase I (Thermo Scientific TM , Waltham, MA, USA). Total RNA (1 µg) was combined with SuperScript III TM Reverse Transcriptase (Invitrogen TM , Carlsbad, CA, USA), 50 µM oligo(dT) 20 and 10 mM dNTPs in 20 µL cDNA synthesis reactions. Quantitative real-time PCR was carried out with PerfeCTa®SYBR®Green SuperMix, Low ROX TM (Quantabio, Beverly, MA, USA) on a Quant Studio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA).…”

Section: Generation and Confirmation Of Esyn1 Knockout And Overexpresmentioning

confidence: 99%

“…The gradient initiated at 60% solvent B held for 1 min, increased to 95% over 2 min, held at 95% for 1.5 min, returned to starting conditions over 0.5 min, and allowed to equilibrate for 3.5 min. The HRMS was operated in ESI + mode monitoring m/z 100-1000 using the following parameters: sheath gas (20), auxiliary gas (5), sweep gas (0), spray voltage (4.2 kV), capillary temperature (320 • C), capillary voltage (35 V), tube lens (100 V), maximum injection time (500 ms), and microscans (1). Targeted enniatin analog [M + H]+ pseudomolecular ions were called into the ion trap with isolation windows of 5 m/z (to allow for spectral accuracy determination of fragment ions) and MS2 scans were acquired using CID at 35 eV.…”

Section: Metabolic Profilingmentioning

confidence: 99%

See 1 more Smart Citation

Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas

et al. 2020

View full text Add to dashboard Cite

Fusarium avenaceum is a generalist pathogen responsible for diseases in numerous crop species. The fungus produces a series of mycotoxins including the cyclohexadepsipeptide enniatins. Mycotoxins can be pathogenicity and virulence factors in various plant–pathogen interactions, and enniatins have been shown to influence aggressiveness on potato tubers. To determine the role of these mycotoxins in other F. avenaceum–host interactions, ENNIATIN SYNTHASE 1 (ESYN1) disruption and overexpression mutants were generated and their ability to infect wheat and peas investigated. As a preliminary study, the transformants were screened for their ability to cause potato tuber necrosis and, consistent with a previous report, enniatin production increased necrotic lesion size on the tubers. By contrast, when the same mutants were assessed in their ability to cause disease in pea roots or durum wheat spikes, no changes in disease symptoms or virulence were observed. While it is known that, at least in the case of wheat, exogenously applied enniatins can cause tissue necrosis, this group of mycotoxins does not appear to be a key factor on its own in disease development on peas or durum wheat.

show abstract

Section: Generation and Confirmation Of Esyn1 Knockout And Overexpresmentioning

confidence: 99%

Section: Metabolic Profilingmentioning

confidence: 99%

Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The genus Fusarium comprises several species that can cause severe root rot impeding cultivation of pea and common bean worldwide (Coleman, 2016;Hwang, Chang, Strelkov, Gossen, & Howard, 2014). For instance, Fusarium solani and Fusarium avenaceum were among the most frequently isolated pathogens in the most important pea production regions of North America (Chittem et al, 2015;Feng et al, 2009;Taheri et al, 2017). Yield losses were reported to reach up to 57% and 84% for pea and common bean, respectively (Basu, Brown, Crête, & Al, 1976;Schneider, Grafton, & Kelly, 2001).…”

Section: Major Root Diseases Of Grain Legumesmentioning

confidence: 99%

“…There are also reports of lentil fields in North America being affected by A. euteiches (Vandemark & Porter, 2010 In the past, much research has been focused on single pathogen species and their life cycles and infection strategies have been thoroughly summarized (Allen & Lenné, 1997;Gaulin et al, 2007;Nzungize & Lyumugabe, 2012). Recent research in legume diseases has increasingly focused on the co-occurrence of various pathogens as complexes Chittem et al, 2015;Taheri et al, 2017). Although many pathogens of legumes have a global distribution, regional structuring of abundance exists.…”

Section: Major Root Diseases Of Grain Legumesmentioning

confidence: 99%

Insights to plant–microbe interactions provide opportunities to improve resistance breeding against root diseases in grain legumes

Wille

Messmer

Studer

et al. 2018

Plant Cell & Environment

115

View full text Add to dashboard Cite

Root and foot diseases severely impede grain legume cultivation worldwide. Breeding lines with resistance against individual pathogens exist, but these resistances are often overcome by the interaction of multiple pathogens in field situations. Novel tools allow to decipher plant-microbiome interactions in unprecedented detail and provide insights into resistance mechanisms that consider both simultaneous attacks of various pathogens and the interplay with beneficial microbes. Although it has become clear that plant-associated microbes play a key role in plant health, a systematic picture of how and to what extent plants can shape their own detrimental or beneficial microbiome remains to be drawn. There is increasing evidence for the existence of genetic variation in the regulation of plant-microbe interactions that can be exploited by plant breeders. We propose to consider the entire plant holobiont in resistance breeding strategies in order to unravel hidden parts of complex defence mechanisms. This review summarizes (a) the current knowledge of resistance against soil-borne pathogens in grain legumes, (b) evidence for genetic variation for rhizosphere-related traits, (c) the role of root exudation in microbe-mediated disease resistance and elaborates (d) how these traits can be incorporated in resistance breeding programmes.

show abstract

“…Over the past few years, several alfalfa genes have been reported to play important roles in resistance against Fusarium spp. (Román-Avilés and Kelly 2005;Vitale et al 2014;Chittem et al 2015), even though the detailed functions of these genes were not fully understood. However, on protein level, studies on alfalfa root rot disease resistance are rather rare.…”

Section: Introductionmentioning

confidence: 99%

Modulation of protein expression in alfalfa (Medicago sativa L.) root and leaf tissues by Fusarium proliferatum

Cong

Sun

Long

et al. 2017

Journal of Integrative Agriculture

View full text Add to dashboard Cite

Alfalfa (Medicago sativa L.) is an important forage crop and is also a target of many fungal diseases including Fusarium spp. As of today, very little information is available about molecular mechanisms that contribute to pathogenesis and defense responses in alfalfa against Fusarium spp. and specifically against Fusarium proliferatum, the causal agent of alfalfa root rot. In this study, we used a proteomic approach to identify inducible proteins in alfalfa during a compatible interaction with F. proliferatum strain YQC-L1. Samples used for the two-dimensional gel electrophoresis (2-DE) and MALDI-TOF/TOF mass spectrometry were from roots and leaves of alfalfa cultivar AmeriGraze 401+Z and WL656HQ. Plants were grown in hydroponic conditions and at 4 days post inoculation with YQC-L1. Our disease symptom assays indicated that AmeriGraze 401+Z was tolerant to YQC-L1 infection while WL656HQ was highly susceptible. Analysis of differentially expressed proteins found in the 2-DE was further characterized using the MASCOT MS/MS ion search software and associated databases to identify multiple proteins that might be involved in F. proliferatum resistance. A total of 66 and 27 differentially expressed proteins were found in the roots and leaves of the plants inoculated with YQC-L1, respectively. These identified proteins were placed in various categories including defense and stress response related metabolism, photosynthesis and protein synthesis. Thirteen identified proteins were validated for their expressions by quantitative reverse transcription (qRT)-PCR. Our results suggested that some of the identified proteins might play important roles in alfalfa resistance against Fusarium spp. These finding could facilitate further dissections of molecular mechanisms controlling root rot disease in alfalfa and potentially other legume crops.

show abstract

Identification and characterization of Fusarium spp. associated with root rots of field pea in North Dakota

Cited by 63 publications

References 18 publications

Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas

Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas

Insights to plant–microbe interactions provide opportunities to improve resistance breeding against root diseases in grain legumes

Modulation of protein expression in alfalfa (Medicago sativa L.) root and leaf tissues by Fusarium proliferatum

Contact Info

Product

Resources

About