Identification and characterization of
            <i>Sr13</i>
            , a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group

Zhang, Wenjun; Chen, Shisheng; Abate, Zewdie; Nirmala, Jayaveeramuthu; Rouse, Matthew N.; Dubcovsky, Jorge

doi:10.1073/pnas.1706277114

Cited by 157 publications

(216 citation statements)

References 50 publications

Supporting

Mentioning

212

Contrasting

Unclassified

Order By: Relevance

“…Thousands of FVs evolved in Ethiopia with the simultaneous contribution of natural and artificial selection, following a complex pattern of trait selection and amplification (Kidane et al, 2017b;Mancini et al, 2017). Therefore, the Ethiopian durum wheat is a source of several adaptive traits of breeding relevance, including disease resistance alleles to rusts and Septoria (Kidane et al, 2017a;Liu et al, 2017;Zhang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat

Kidane

Gesesse

Hailemariam

et al. 2019

Plant Biotechnology Journal

View full text Add to dashboard Cite

Summary The Ethiopian plateau hosts thousands of durum wheat ( Triticum turgidum subsp. durum ) farmer varieties ( FV ) with high adaptability and breeding potential. To harness their unique allelic diversity, we produced a large nested association mapping ( NAM ) population intercrossing fifty Ethiopian FV s with an international elite durum wheat variety (Asassa). The Ethiopian NAM population (Et NAM ) is composed of fifty interconnected bi‐parental families, totalling 6280 recombinant inbred lines ( RIL s) that represent both a powerful quantitative trait loci ( QTL ) mapping tool, and a large pre‐breeding panel. Here, we discuss the molecular and phenotypic diversity of the Et NAM founder lines, then we use an array featuring 13 000 single nucleotide polymorphisms ( SNP s) to characterize a subset of 1200 Et NAM RIL s from 12 families. Finally, we test the usefulness of the population by mapping phenology traits and plant height using a genome wide association ( GWA ) approach. Et NAM RIL s showed high allelic variation and a genetic makeup combining genetic diversity from Ethiopian FV s with the international durum wheat allele pool. Et NAM SNP data were projected on the fully sequenced AB genome of wild emmer wheat, and were used to estimate pairwise linkage disequilibrium ( LD ) measures that reported an LD decay distance of 7.4 Mb on average, and balanced founder contributions across Et NAM families. GWA analyses identified 11 genomic loci individually affecting up to 3 days in flowering time and more than 1.6 cm in height. We argue that the Et NAM is a powerful tool to support the production of new durum wheat varieties targeting local and global agriculture.

show abstract

Section: Introductionmentioning

confidence: 99%

A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat

Kidane

Gesesse

Hailemariam

et al. 2019

Plant Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…tauschii. Given stem rust resistance genes are known to encode nucleotide-binding site and leucine rich repeat (NLR) proteins [33–35], and the QTL identified from Ae. umbellulata bi- parental populations fits into features of a qualitative gene, exploring the presence of NLR genes in the predicted region is useful to identify short lists of potential candidate genes.…”

Section: Discussionmentioning

confidence: 99%

Bulked segregant analysis RNA-seq (BSR-Seq) validated a stem resistance locus in Aegilops umbellulata, a wild relative of wheat

Edae

Rouse

2019

Preprint

Self Cite

View full text Add to dashboard Cite

11Many disease resistance genes that have been transferred from wild relatives to cultivated wheat 12 have played a significant role in wheat production worldwide. Ae. umbellulata is one of the 13 species within the genus Aegilops that have been successfully used as sources of resistance genes 14 to leaf rust, stem rust and powdery mildew. The objectives of the current work was to validate 15 the map position of a major QTL that confers resistance to the stem rust pathogen races Ug99 16 (TTKSK) and TTTTF with an independent bi-parental mapping population and to refine the 17 QTL region with a bulk segregant analysis approach. Two F 2 bi-parental mapping populations 18 were developed from stem rust resistant Ae. umbellulata accessions (PI 298905 and PI 5422375) 19 and stem rust susceptible accessions (PI 542369 and PI 554395). Firstly, one of the two 20 populations was used to map the chromosome location of the resistance gene. Later on, the 2 nd 21 population was used to validate the chromosome location in combination with a bulk segregant 22 analysis approach. For the bulk segregant analysis, RNA was extracted from a bulk of leaf 23 tissues of 12 homozygous resistant F 3 families, and a separate bulk of 11 susceptible 2 24 homozygous F 3 families derived from the PI 5422375 and PI 554395 cross. The RNA samples of 25 the two bulks and the two parents were sequenced for SNPs identification. Stem rust resistance 26 QTL was validated on chromosome 2U of Ae. umbellulata in the same region in both 27 populations. With bulk segregant analysis, the QTL position was delimited within 3.2 Mbp. 28Although there were a large number of genes in the orthologous region of the detected QTL on 29 chromosome 2D of Ae. tauschii, we detected only two Ae. umbellulata NLR genes which can be 30 considered as a potential candidate genes. 31 32 3 33 Introduction 34 Wheat (Triticum aestivum L.) is used as a main source of protein and starch for human 35consumption. However, its production is threatened by many diseases including stem rust caused 36 by Puccinia graminis f. sp. tritici (Pgt), which is known to cause severe yield losses in 37 susceptible cultivars. Ug99, the stem rust race group discovered in Uganda in 1998, was found to 38 be virulent on widely deployed resistance genes, and the majority of the world's wheat 39 germplasm is susceptible to this race group [1]. The emergence and continued evolution of Ug99 40 has pressed the research community to evaluate available genetic resources for resistance and 41 rapidly develop Ug99-resistant cultivars. Since the cultivated wheat gene pool has a narrow 42 genetic base for resistance to Ug99 and up to 90% of world's wheat cultivars are considered 43 Ug99 susceptible [2], identifying resistance genes from wild relatives and introgressing them 44 into cultivated wheat is a tractable strategy for improving disease resistance. 45 Many wild relatives of cultivated wheats have been used as sources of disease resistance genes 46 over the last century, and those deployed genes have a...

show abstract

“…However, efficient gene stewardship measures need to be adopted to limit the occurrence of boom-bust cycles. Even though the wheat genome is large and complex, significant progress has been made in cloning six Ug99 resistance genes, including Sr13, Sr22, Sr33, Sr45, Sr35 and Sr50 [57][58][59][60][61] . More resistance genes are necessary to diversify the combinations of resistance genes deployed as gene pyramids, or in transgenic cassettes, to provide durable resistance [25] .…”

Section: Race-specific Resistance Genesmentioning

confidence: 99%

Progress in breeding for resistance to Ug99 and other races of the stem rust fungus in CIMMYT wheat germplasm

Bhavani¹,

Hodson²,

Espino³

et al. 2019

Front. Agr. Sci. Eng.

View full text Add to dashboard Cite

show abstract

Identification and characterization of Sr13 , a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group

Cited by 157 publications

References 50 publications

A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat

A large nested association mapping population for breeding and quantitative trait locus mapping in Ethiopian durum wheat

Bulked segregant analysis RNA-seq (BSR-Seq) validated a stem resistance locus in Aegilops umbellulata, a wild relative of wheat

Progress in breeding for resistance to Ug99 and other races of the stem rust fungus in CIMMYT wheat germplasm

Contact Info

Product

Resources

About