Identification and characterization of limbal stem cells

Schlötzer‐Schrehardt, Ursula; Kruse, Friedrich E.

doi:10.1016/j.exer.2005.02.016

Cited by 413 publications

(362 citation statements)

References 87 publications

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“…It is highly plausible that limbal epithelial SCs may express a set of genes as their "signature" rather than a single one, similar to what has been found for epidermal keratinocyte SCs [71, npg 73]. Both the label-retaining pattern and immunostaining patterns of several putative markers have revealed that not all basal cells are SCs (for review see [44]), a finding consistent with the notion that true SCs represent only a small fraction in a given tissue [74]. Taken together, the heterogeneity of limbal basal cells also suggests that limbal SCs are physically in close contact with their immediate progeny, i.e., TACs.…”

Section: How Can Limbal Epithelial Scs and Their Niche Cells Be Identmentioning

confidence: 72%

“…The other method to identify limbal epithelial SCs is based on their expressed markers. Although limbal epithelial SCs preferentially express several markers (see above), unfortunately there is no consensus bona fide marker [44,45]. It is highly plausible that limbal epithelial SCs may express a set of genes as their "signature" rather than a single one, similar to what has been found for epidermal keratinocyte SCs [71, npg 73].…”

Section: How Can Limbal Epithelial Scs and Their Niche Cells Be Identmentioning

confidence: 74%

“…It also remains to be determined whether limbal TACs, like those in the cardiac niche [72], may play a role in instructing the SC fate in the niche. Intriguingly, some limbal basal cells also express vimentin [18,44]…”

Section: How Can Limbal Epithelial Scs and Their Niche Cells Be Identmentioning

confidence: 99%

“…These new advances collectively make the corneal/limbal epithelia a prime tissue to practice regenerative medicine. For more detailed information on the aforementioned progresses regarding limbal epithelial SCs, the reader is encouraged to consult several reviews [35][36][37][38][39][40][41][42][43][44][45]. Further understanding of how self-renewal and fate decision of limbal epithelial SCs are regulated will undoubtedly unravel their additional therapeutic potentials in the future.…”

Section: Introductionmentioning

confidence: 99%

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Niche regulation of corneal epithelial stem cells at the limbus

et al. 2007

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Among all adult somatic stem cells, those of the corneal epithelium are unique in their exclusive location in a defined limbal structure termed Palisades of Vogt. As a result, surgical engraftment of limbal epithelial stem cells with or without ex vivo expansion has long been practiced to restore sights in patients inflicted with limbal stem cell deficiency. Nevertheless, compared to other stem cell examples, relatively little is known about the limbal niche, which is believed to play a pivotal role in regulating self-renewal and fate decision of limbal epithelial stem cells. This review summarizes relevant literature and formulates several key questions to guide future research into better understanding of the pathogenesis of limbal stem cell deficiency and further improvement of the tissue engineering of the corneal epithelium by focusing on the limbal niche.

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Section: How Can Limbal Epithelial Scs and Their Niche Cells Be Identmentioning

confidence: 72%

Section: How Can Limbal Epithelial Scs and Their Niche Cells Be Identmentioning

confidence: 74%

Section: How Can Limbal Epithelial Scs and Their Niche Cells Be Identmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Niche regulation of corneal epithelial stem cells at the limbus

et al. 2007

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“…Transmission electron microscopy showed small-roundish cells at the bottom of the palisade rete ridges with large nuclei containing heterochromatin-rich DNA and a barely detectable nucleolus, scarce cytoplasm with delicate melanin granules and a fine basement membrane through which cytoplasmic invaginations from the underlying stroma could be observed (38,39). In a recent study, cells with the smallest size (10-16 lm) expressed the highest amount of the putative stem cell markers DNp63 and ABCG2, both at mRNA and protein levels, included among themselves the most label-retaining (side population) cells, and had the highest clonogenic capacity in culture (40).…”

Section: Characteristics Of Limbal Epithelial Stem Cellsmentioning

confidence: 99%

Stem cells of the adult cornea: From cytometric markers to therapeutic applications

et al. 2008

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The cornea is a major protective shield of the interior of the eye and represents two thirds of its refractive power. It is made up of three tissue layers that have different developmental origins: the outer, epithelial layer develops from the ectoderm overlying the lens vesicle, whereas the stroma and the endothelium have mesenchymal origin. In the adult organism, the outermost corneal epithelium is the most exposed to environmental damage, and its constant renewal is assured by the epithelial stem cells that reside in the limbus, the circular border of the cornea. Cell turnover in the stromal layer is very slow and the endothelial cells probably do not reproduce in the adult organism. However, recent experimental evidence indicates that stem cells may be found in these layers. Damage to any of the corneal layers leads to loss of transparency and low vision. Corneal limbal stem cell deficiency results in severe ocular surface disease and its treatment by transplantating ex vivo expanded limbal epithelial cells is becoming widely accepted today. Stromal and endothelial stem cells are potential tools of tissue engineering and regenerative therapies of corneal ulcers and endothelial cell loss. In the past few years, intensive research has focused on corneal stem cells aiming to improve the outcomes of the current corneal stem cell transplantation techniques. This review summarizes the current state of knowledge on corneal epithelial, stromal and endothelial stem cells. Special emphasis is placed on the molecular markers that may help to identify these cells, and the recently revealed mechanisms that could maintain their ''stemness'' or drive their differentiation. The techniques for isolating and culturing/ expanding these cells are also described. '

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Culture of Human Corneal Stem Cells

Funderburgh

2007

Culture of Human Stem Cells

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Identification and characterization of limbal stem cells

Cited by 413 publications

References 87 publications

Niche regulation of corneal epithelial stem cells at the limbus

Niche regulation of corneal epithelial stem cells at the limbus

Stem cells of the adult cornea: From cytometric markers to therapeutic applications

Culture of Human Corneal Stem Cells

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