Identification and characterization of MYC transcription factors in Taxus sp.

Yang, Yongqiang; Zhang, Kaikai; Yang, Li‐Tao; Lv, Xing; Wu, Ying; Liu, Hongwei; Lu, Qiang; Chen, Duanfen; Qiu, Deyou

doi:10.1016/j.gene.2018.06.065

Cited by 44 publications

(28 citation statements)

References 44 publications

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“…The differential expressions of the above two important metabolic pathways might provide a number of genes that were involved in the interspecific differential accumulations of taxol and its derivatives. In the taxol biosynthetic pathway, α- and β-phenylalanine are involved in the side chain assembly [32, 33]. KEGG analysis showed that the phenylalanine metabolism pathway was siginificantly enriched in all three comparisons, suggesting an involvement of side chain assembly in interspecific differential accumulations of taxol.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome analyses provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species

Zhou

Luo

et al. 2019

BMC Plant Biol

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BackgroundTaxol is an efficient anticancer drug; however, the accumulation of taxoids can vary hugely among Taxus species. The mechanism underlying differential accumulation of taxoids is largely unknown. Thus, comparative analysis of the transcriptomes in three Taxus species, including T. media, T. mairei and T. cuspidata, was performed.ResultsKEGG enrichment analysis revealed that the diterpenoid biosynthesis and cytochrome P450 pathways were significantly enriched in different comparisons. Differential expressions of these taxol biosynthesis related genes might be a potential explanation for the interspecific differential accumulation of taxol and its derivatives. Besides, the sequences of several MEP pathway-associated genes, such as DXS, DXR, MCT, CMK, MDS, HDS, HDR, IPPI, and GGPPS, were re-assembled based on independent transcriptomes from the three Taxus species. Phylogenetic analysis of these MEP pathway-associated enzymes also showed a high sequence similarity between T. media and T. cuspidata. Moreover, 48 JA-related transcription factor (TF) genes, including 10 MYBs, 5 ERFs, 4 RAPs, 3 VTCs, and 26 other TFs, were analyzed. Differential expression of these JA-related TF genes suggested distinct responses to exogenous JA applications in the three Taxus species.ConclusionsOur results provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species. The data give us an opportunity to reveal the mechanism underlying the variations in the taxoid contents and to select the highest-yielding Taxus species.Electronic supplementary materialThe online version of this article (10.1186/s12870-019-1645-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

“…Furthermore, increasing evidences showed that various TF families, such as bHLH and WRKY, involved in the regulation of taxol biosynthesis [32]. For example, TcMYC1, TcMYC2, and TcMYC4 are involved in transduction of JA signals and regulation of taxol biosynthesis [44, 45].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analyses provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species

Zhou

Luo

et al. 2019

BMC Plant Biol

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“…The binding of MYC transcription factors to the G-box has also been shown for two very distinct tree species, Taxus sp. and Hevea brasiliensis [49, 50] . The more general role of the G-box, however, remains to be characterized in trees.…”

Section: Discussionmentioning

confidence: 99%

RNA-seq of eight different poplar clones reveals conserved up-regulation of gene expression in response to insect herbivory

Müller¹,

Kersten²,

Fladung³

et al. 2019

BMC Genomics

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Background: Herbivorous insects can have a profound impact on plant growth performance. In some years, canopy damage in poplar plantations exceeds 50% of the total leaf surface, thereby possibly compromising carbon fixation and biomass yield. To assess the transcriptional response of elite poplar clones to insect feeding and to test whether this response varies between different genotypes, we performed an RNA-sequencing experiment. We deeply sequenced the transcriptomes of eight elite clones belonging to three poplar species (Populus trichocarpa, P. nigra and P. maximowiczii), under Phratora vitellinae feeding and control conditions. This allowed us to precisely quantify transcript levels of about 24,000 expressed genes. Results: Our data reveal a striking overall up-regulation of gene expression under insect attack in all eight poplar clones studied. The up-regulated genes were markedly enriched for the biological process 'regulation of transcription' indicating a highly concerted restructuring of the transcriptome. A search for potential cis-regulatory elements (CREs) that may be involved in this process identified the G-box (CACGTG) as the most significant motif in the promoters of the induced genes. In line with the role of the G-box in jasmonate (JA)-mediated activation of gene expression by MYC2, several genes involved in JA biosynthesis and signaling were up-regulated in our dataset. A co-expression network analysis additionally highlighted WRKY transcription factors. Within the most prominent expression module, WRKYs were strongly overrepresented and occupied several network hubs. Finally, the insect-induced genes comprised several protein families known to be involved in plant defenses, e.g. cytochrome P450s, chitinases and protease inhibitors. Conclusions: Our data represent a comprehensive characterization of the transcriptional response of selected elite poplar clones to insect herbivory. Our results suggest that the concerted up-regulation of gene expression is controlled by JA signaling and WRKY transcription factors, and activates several defense mechanisms. Our data highlight potential targets of selection and may thus contribute to breeding insect-resistant poplar clones.

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“…Studies in different plant species demonstrated that MYC2 transcription was induced by MeJA treatment, e.g., in apple ( Malus x domestica ) [ 110 ] and yew-tree ( Taxus sp.) [ 111 ].…”

Section: Discussionmentioning

confidence: 99%

Methyl Jasmonate Affects Photosynthesis Efficiency, Expression of HvTIP Genes and Nitrogen Homeostasis in Barley

Kurowska

Daszkowska‐Golec

Gajecka

et al. 2020

IJMS

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Jasmonates modulate many growth and developmental processes and act as stress hormones that play an important role in plant tolerance to biotic and abiotic stresses. Therefore, there is a need to identify the genes that are regulated through the jasmonate signalling pathway. Aquaporins, and among them the Tonoplast Intrinsic Proteins (TIPs), form the channels in cell membranes that are responsible for the precise regulation of the movement of water and other substrates between cell compartments. We identified the cis-regulatory motifs for the methyl jasmonate (MeJA)-induced genes in the promoter regions of all the HvTIP genes, which are active in barley seedlings, and thus we hypothesised that the HvTIP expression could be a response to jasmonate signalling. In the presented study, we determined the effect of methyl jasmonate on the growth parameters and photosynthesis efficiency of barley seedlings that had been exposed to different doses of MeJA (15–1000 µM × 120 h) in a hydroponic solution. All of the applied MeJA concentrations caused a significant reduction of barley seedling growth, which was most evident in the length of the first leaf sheath and dry leaf weight. The observed decrease of the PSII parameters after the exposure to high doses of MeJA (500 µM or higher) was associated with the downregulation of HvPsbR gene encoding one of the extrinsic proteins of the Oxygen Evolving Complex. The reduced expression of HvPsbR might lead to the impairment of the OEC action, manifested by the occurrence of the K-band in an analysis of fluorescence kinetics after MeJA treatment as well as reduced photosynthesis efficiency. Furthermore, methyl jasmonate treatment caused a decrease in the nitrogen content in barley leaves, which was associated with an increased expression the four tonoplast aquaporin genes (HvTIP1;2, HvTIP2;2, HvTIP4;1 and HvTIP4;2) predicted to transport the nitrogen compounds from the vacuole to the cytosol. The upregulation of the nitrogen-transporting HvTIPs might suggest their involvement in the vacuolar unloading of ammonia and urea, which both could be remobilised when the nitrogen content in the leaves decreases. Our research provides tips on physiological role of the individual TIP subfamily members of aquaporins under methyl jasmonate action.

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Identification and characterization of MYC transcription factors in Taxus sp.

Cited by 44 publications

References 44 publications

Transcriptome analyses provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species

Transcriptome analyses provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species

RNA-seq of eight different poplar clones reveals conserved up-regulation of gene expression in response to insect herbivory

Methyl Jasmonate Affects Photosynthesis Efficiency, Expression of HvTIP Genes and Nitrogen Homeostasis in Barley

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