Identification and characterization of nine polymorphic microsatellite loci in the North American pika, <i>Ochotona princeps</i>

Peacock, Mary M.; Kirchoff, Veronica S.; Merideth, Susan J.

doi:10.1046/j.1471-8286.2002.00249.x

Cited by 47 publications

(48 citation statements)

References 9 publications

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“…Four SSR-enriched genomic libraries were constructed by Genetic Identification Services, Chatsworth, Calif., (GIS; http://www.genetic-id-services.com) using a magnetic bead-capture approach (Peacock et al 2002). Biotin-(CA) 15 , biotin-(GA) 15 , biotin-(AAT) 12 , and biotin-(ATG) 12 were used as capture molecules for the four libraries, respectively.…”

Section: Genomic Libraries and Isolation Of Ssrsmentioning

confidence: 99%

Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations

et al. 2003

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Twenty-two highly variable SSR markers were developed in Douglas-fir [ Pseudotsuga menziesii (Mirb.) Franco] from five SSR-enriched genomic libraries. Fifteen PCR primer pairs amplified a single codominant locus, while seven primer pairs occasionally amplified two loci. The Mendelian inheritance of all 22 SSRs was confirmed via segregation analyses in several Douglas-fir families. The mean observed heterozygosity and the mean number of alleles per locus were 0.855 (SE=0.020) and 23 (SE=1.6), respectively. Twenty markers were used in genetic linkage analysis and mapped to ten known linkage groups. Because of their high polymorphism and unambiguous phenotypes, 15 single-locus markers were selected as the most suitable for DNA fingerprinting and parentage analysis. Only three SSRs were sufficient to achieve an average probability of exclusion from paternity of 0.998 in a Douglas-fir seed orchard block consisting of 59 parents.

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Section: Genomic Libraries and Isolation Of Ssrsmentioning

confidence: 99%

Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations

et al. 2003

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“…Three microsatellite-enriched libraries were prepared for G. muhlenbergii by Genetic Identification Services Inc., Chatsworth, CA, USA (GIS; http://www.genetic-id-services.com/) using magnetic bead capture technology and CA (A library), ATG (B library), and TAGA (D library) microsatellite motif capture molecules (Peacock et al 2002). A total of 244 positive colonies were selected for marker development from the three enriched libraries: A ¼ 32, B ¼ 91, and D ¼ 121.…”

mentioning

confidence: 99%

Conservation of microsatellite DNA flanking sequence across 13 Emydid genera assayed with novel bog turtle (Glyptemys muhlenbergii) loci

King¹,

Julian²

2004

Conservation Genetics

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“…Specifically, we designed species-specific primers to amplify mitochondrial cytochrome b fragments of 250 and 800 base pairs (bp; Table S1) and used one primer previously described for the genus Ochotona (Yu et al 2000). Likewise, nuclear DNA amplification was tested at two microsatellite loci previously described for the American pika, Ocp8 (250 bp) and Ocp2 (400 bp) (Peacock et al 2002). PCRs were performed using a Veriti® thermal cycler (Applied Biosystems, Foster City, CA, USA) in a 25-μl volume containing: 1-20 ng DNA, 0.5 μM of each primer, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl 2 , 200 μM dNTPs, 10 μg bovine serum albumin (BSA; New England Biolabs, Ipswich, MA, USA) and 0.5 U AmpliTaq Gold® DNA polymerase (Applied Biosystems).…”

Section: Dna Isolation and Pcr Amplificationmentioning

confidence: 99%

Obtaining high-quality DNA from elusive small mammals using low-tech hair snares

Henry

Russello

2010

Eur J Wildl Res

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Noninvasive sampling approaches are becoming increasingly important for enabling genetic studies of wildlife populations. While a number of methods have been described to noninvasively sample hair from carnivores and medium-sized mammals, they have largely remained untested in elusive small mammals. Here we describe a novel and inexpensive noninvasive hair snare targeted at an elusive small mammal, the American pika (Ochotona princeps). We explore the quality of the sample by assessing PCR amplification success of mitochondrial and nuclear DNA fragments across four commercially available DNA isolation kits and two different quantities of hair in a factorial design. Additionally, we determined the sex of the individual samples using PCR-RFLP of ZFX/ ZFY loci. We found that our snare is effective in obtaining hair that yield DNA of sufficient quality and quantity to successfully amplify a range of mitochondrial and nuclear fragment sizes. Specifically, we found the greatest success in amplifying mitochondrial DNA, nuclear microsatellites and ZFX/ZFY loci using at least 25 hairs as starting material and the DNA IQ™ system. The hair snares thus provide a cost-effective and minimally intrusive approach to sample elusive or rare small mammals. We anticipate that this approach will be useful to obtain samples for molecular studies of the ecology, evolution and conservation of small, elusive mammals.

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Identification and characterization of nine polymorphic microsatellite loci in the North American pika, Ochotona princeps

Cited by 47 publications

References 9 publications

Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations

Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations

Conservation of microsatellite DNA flanking sequence across 13 Emydid genera assayed with novel bog turtle (Glyptemys muhlenbergii) loci

Obtaining high-quality DNA from elusive small mammals using low-tech hair snares

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