Identification and Characterization of OsWRKY72 Variant in Indica Genotypes

Ashwini, Narasimha; Sajeevan, Radha Sivarajan; Udayakumar, M.; Nataraja, K.

doi:10.1016/j.rsci.2016.07.002

Cited by 8 publications

(6 citation statements)

References 26 publications

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“…Between Mulai and IR29, there were more types of DEGs involved in transcription control that were identified in IR29, and the majority of them are highly regulated by salinity stress treatment. Among the types of transcription factors identified in this study are comparable to previously identified factors such as OsNAC45 (Zhang et al 2020), OsMYB6 (Tang et al 2019), OsDREB2A (Zhang et al 2013), and OsWRKY72 (Ashwini et al 2016), all with clear participation in salinity response and tolerance in rice. Transcription factors play a number of roles in salinity stress response in plants which include signaling for hormone metabolism such as ABA exhibited by OsMYB48‐1 (Xiong et al 2014), OsMADS25 (Xu et al 2018), and OsNAC45 (Zhang et al 2020); maintenance of cellular redox homeostasis as with OsSTAP1 (Wang et al 2020) and OsDREB2A (Zhang et al 2013); and, sustaining growth as exemplified by OsMADS25 (Xu et al 2018) and OsMYB91 (Zhu et al 2015).…”

Section: Discussionsupporting

confidence: 87%

Comparative transcriptome analysis of root types in salt tolerant and sensitive rice varieties in response to salinity stress

Cartagena

Yao

Mitsuya

et al. 2021

Physiologia Plantarum

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Salinity tolerance in rice is a very important trait, especially in areas that are affected by soil salinity, such as tsunami‐devastated areas and coastal regions in rice‐producing countries. The roots are the key organs that first detect and respond to salinity stress; thus, it is important to have an understanding of how roots contribute to salinity tolerance in agricultural crops. After salinity treatment of the salt tolerant (Mulai) and sensitive (IR29) rice varieties, it appeared that among the three types of roots, the L‐type lateral roots (LLR) were the most sensitive to salinity stress in Mulai and the most tolerant in IR29. The nodal roots (NR) and the S‐type lateral roots (SLR) were all negatively affected by salinity treatment in both rice varieties. In order to elucidate the molecular mechanism of the difference in stress response among rice root types, the RNA‐seq transcriptome profiles of NR, LLR, and SLR were analyzed in Mulai and IR29. Between the two rice varieties, more transporters were found to participate in the regulation of salt tolerance in Mulai roots, such as those involved in ion and sugar transport. In IR29, many of the genes detected were associated with transcription regulation, including stress‐inducible genes such as NAC, WRKY and MYB. Among the different root types, gene expression in LLR and SLR were significantly regulated in both rice varieties. Taken together, the genes identified in this study may be utilized in the varietal improvement of rice with very specific root traits that can enhance tolerance to salinity stress.

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Section: Discussionsupporting

confidence: 87%

Comparative transcriptome analysis of root types in salt tolerant and sensitive rice varieties in response to salinity stress

Cartagena

Yao

Mitsuya

et al. 2021

Physiologia Plantarum

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“…This was agreement with the literature revealing WRKY7 improved Cd tolerance through increase antioxidant enzymes SOD and peroxidase (POD) in Arabidopsis [ 51 ]. Similarly, the phosphorylation of WRKY72 has been associated with heightened salinity stress tolerance in rice [ 52 ]. Another class of transcription factor, the so-called Zn finger CCCH domain-containing proteins, has been implicated in cellular development and the abiotic stress response [ 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

The Phosphoproteomic Response of Rice Seedlings to Cadmium Stress

Zhong

Liu

Huang

et al. 2017

IJMS

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The environmental damage caused by cadmium (Cd) pollution is of increasing concern in China. While the overall plant response to Cd has been investigated in some depth, the contribution (if any) of protein phosphorylation to the detoxification of Cd and the expression of tolerance is uncertain. Here, the molecular basis of the plant response has been explored in hydroponically raised rice seedlings exposed to 10 μΜ and 100 μΜ Cd2+ stress. An analysis of the seedlings’ quantitative phosphoproteome identified 2454 phosphosites, associated with 1244 proteins. A total of 482 of these proteins became differentially phosphorylated as a result of exposure to Cd stress; the number of proteins affected in this way was six times greater in the 100 μΜ Cd2+ treatment than in the 10 μΜ treatment. A functional analysis of the differentially phosphorylated proteins implied that a significant number was involved in signaling, in stress tolerance and in the neutralization of reactive oxygen species, while there was also a marked representation of transcription factors.

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“…OsWRKY72 was upregulated in the salt‐tolerant indica genotype in the first hours of salt stress (Ashwini et al . 2016). Also, OsWRKY72 is responsive to salt, ABA and IAA in japonica rice (Ramamoorthy et al .…”

Section: Resultsmentioning

confidence: 99%

“…The gene OsWRKY72 is reported to respond to abiotic stresses. OsWRKY72 was upregulated in the salt-tolerant indica genotype in the first hours of salt stress (Ashwini et al 2016). S1.…”

Section: Potential Negative Regulators Of Chilling Tolerancementioning

confidence: 99%

When rice gets the chills: comparative transcriptome profiling at germination shows WRKY transcription factor responses

et al. 2021

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Rice is vital for food security. Due to its tropical origin, rice suffers from cold temperatures that affect its entire life cycle. Key genes have been identified involved in cold tolerance. WRKYs are generally downstream of the MAPK cascade and can act together with VQ proteins to regulate stress-responsive genes.• Chilling treatment was applied at germination to two rice genotypes (tolerant and sensitive). Shoots at S3 stage were collected for RNA-seq to identify OsWRKY, OsMAPKs and OsVQs expression. Relationships among MAPKs, WRKYs and VQs were predicted through correlation analysis. OsWRKY transcriptional regulation was predicted by in silico analysis of cis-regulatory elements.• A total of 39 OsWRKYs were differentially expressed. OsWRKY21, OsWRK24 and OsWRKY69 are potential positive regulators, while OsWRKY10, OsWRK47, OsWRKY62, OsWRKY72 and OsWRKY77 are potential negative regulators, of chilling tolerance. 12 OsMAPKs were differentially expressed. OsMAPKs were downregulated and negatively correlated with the upregulated OsWRKYs in the tolerant genotype. 19 OsVQs were differentially expressed, three and six OsVQs were positively correlated with OsWRKYs in the tolerant and sensitive genotypes, respectively. Seven differentially expressed OsWRKYs have cold-responsive elements in their promoters and five upregulated OsWRKYs in the tolerant genotype contained the Wbox motif.• Chilling causes changes in OsWRKY, OsMAPK and OsVQ gene expression at germination. OsWRKYs may not act downstream of the MAPK cascade to coordinate chilling tolerance, but OsWRKYs may act with VQs to regulate chilling tolerance. Candidate OsWRKYs are correlated and have a W-box in the promoter, suggesting an autoregulation mechanism.

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Identification and Characterization of OsWRKY72 Variant in Indica Genotypes

Cited by 8 publications

References 26 publications

Comparative transcriptome analysis of root types in salt tolerant and sensitive rice varieties in response to salinity stress

Comparative transcriptome analysis of root types in salt tolerant and sensitive rice varieties in response to salinity stress

The Phosphoproteomic Response of Rice Seedlings to Cadmium Stress

When rice gets the chills: comparative transcriptome profiling at germination shows WRKY transcription factor responses

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