Identification and characterization of phytoconstituents of ethanolic root extract of Clitoria ternatea L. utilizing HR-LCMS analysis

Abstract

Medicinal plants act as a vital source in improving health and overcoming the side effects of modern-day medicine. Many evidence-based reports are present in the literature about the benefits of medicinal plants. Clitoria ternatea L. belongs to the family Fabaceae and is known to be one of the important Ayurvedic medicinal plant whose uses are specified mainly for the modification of nervous system activities. ‘Medhyarasayana’ is one of the Ayurvedic formulations which is used to promote the intellectual capacity, revive the body and nervous tissue, Clitoria ternatea serves as a major constituent of ‘Medhyarasayana.’ Identification and characterization of active metabolites of C. ternatea will help to isolate the important phytoconstituents responsible for the central nervous system effects, isolated components can be utilized in future for the formulation of new medicine for various neurodegenerative disorders. In the present study, the phytochemical evaluation of the ethanolic root extract of C. ternatea (EECT) was performed using the HR-LCMS technique. Preliminary qualitative phytoconstituents analysis showed the presence of tannins, alkaloids, saponins, steroids, carbohydrate, protein, flavonoids and triterpenoids in the ethanolic root extract. Almost 42 compounds were identified when the EECT subjected to HR-LCMS analysis.

Apoptosis Induction Activity and Inhibition of MCF-7 Cell Proliferation in Active Isolate of Butterfly Pea Flower (Clitoria ternatea L.)

“…Indonesia is one of the countries with a wealth of flora in the world and has various kinds of plants that have the potential for cancer treatment, one of which is the butterfly pea flower plant. The anti-cancer effect of the extract of butterfly pea flower (Clitoria ternatea) has a significant effect on the breast cancer line hormone (MCF-7) [8]. The bioactive components in butterfly pea flower that are thought to have functional benefits are 3 [4,9].…”

Ultrasound‐assisted extraction of bioactive compounds from giloy (Tinospora cordifolia) stem: Quantitative process optimization and bioactives analysis

“…Screening of polyphenols and metabolites using HR-LCMS-QTOF Untargeted screening for polyphenols and metabolites on optimized extracts was performed using High-Resolution Mass Spectrometry Quadruple-Time-of-flight (HR-LCMS-QToF) (G6550A, Agilent Corporation) as described byJiji and Muralidharan (2021) with slight modification. Chromatograph was achieved on binary gradient HPLC system with Hypersil Gold C 18 (50 Â 2.1 mm 2 , 3 μm particle size) column.…”