Identification and Characterization of Porphyromonas gingivalis Client Proteins That Bind to Streptococcus oralis Glyceraldehyde-3-Phosphate Dehydrogenase

Maeda, Kengo; Nagata, Hideki; Kuboniwa, Masae; Ojima, Miki; Osaki, Tsukasa; Minamino, Naoto; Amano, Atsuo

doi:10.1128/iai.00875-12

Cited by 24 publications

(27 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…GAPDH expression was altered in mutants with biofilm deficiencies for Streptococcus suis and Streptococcus mutans (40,41). In Streptococcus oralis, cell surface GAPDH acts as the binding site for biofilm formation by the periodontopathic bacterium Porphyromonas gingivalis (42,43). These studies indicated that GAPDH has a role in bacterial biofilms.…”

Section: Discussionmentioning

confidence: 72%

Genome-Wide Identification of Genes Necessary for Biofilm Formation by Nosocomial Pathogen Stenotrophomonas maltophilia Reveals that Orphan Response Regulator FsnR Is a Critical Modulator

Kang

Wang

Zhang

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

Stenotrophomonas maltophilia is a Gram-negative bacterial pathogen of increasing concern to human health. Most clinical isolates of S. maltophilia efficiently form biofilms on biotic and abiotic surfaces, making this bacterium resistant to a number of antibiotic treatments and therefore difficult to eliminate. To date, very few studies have investigated the molecular and regulatory mechanisms responsible for S. maltophilia biofilm formation. Here we constructed a random transposon insertion mutant library of S. maltophilia ATCC 13637 and screened 14,028 clones. A total of 46 nonredundant genes were identified. Mutants of these genes exhibited marked changes in biofilm formation, suggesting that multiple physiological pathways, including extracellular polysaccharide production, purine synthesis, transportation, and peptide and lipid synthesis, are involved in bacterial cell aggregation. Of these genes, 20 putatively contributed to flagellar biosynthesis, indicating a critical role for cell motility in S. maltophilia biofilm formation. Genetic and biochemical evidence demonstrated that an orphan response regulator, FsnR, activated transcription of at least two flagellum-associated operons by directly binding to their promoters. This regulatory protein plays a fundamental role in controlling flagellar assembly, cell motility, and biofilm formation. These results provide a genetic basis to systematically study biofilm formation of S. maltophilia.

show abstract

Section: Discussionmentioning

confidence: 72%

Genome-Wide Identification of Genes Necessary for Biofilm Formation by Nosocomial Pathogen Stenotrophomonas maltophilia Reveals that Orphan Response Regulator FsnR Is a Critical Modulator

Kang

Wang

Zhang

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Fructose-1,6-bisphosphate aldolase (Fba; PG1755) is an indispensable enzyme in glycolysis, gluconeogenesis, and the TCA cycle (29,30) as well as in both anabolic and catabolic amino acid processes (31). Glyceraldehyde-3-phosphate dehydrogenase (GapA; PG2124), involved in the glycolytic pathway, is responsible for the phosphorylation of glyceraldehyde-3-phosphate (29,32). The last three metabolic enzymes upregulated included 2-oxoglutarate oxidoreductase, subunit gamma (PG1809), acetyl coenzyme A (acetyl-CoA) synthetase (PGN_1117), and 2-ketoisovalerate ferredoxin oxidoreductase (PG1812).…”

Section: Resultsmentioning

confidence: 99%

Protein Analysis of Sapienic Acid-Treated Porphyromonas gingivalis Suggests Differential Regulation of Multiple Metabolic Pathways

et al. 2016

View full text Add to dashboard Cite

Lipids endogenous to skin and mucosal surfaces exhibit potent antimicrobial activity against Porphyromonas gingivalis, an important colonizer of the oral cavity implicated in periodontitis. Our previous work demonstrated the antimicrobial activity of the fatty acid sapienic acid (C 16:1⌬6 ) against P. gingivalis and found that sapienic acid treatment alters both protein and lipid composition from those in controls. In this study, we further examined whole-cell protein differences between sapienic acidtreated bacteria and untreated controls, and we utilized open-source functional association and annotation programs to explore potential mechanisms for the antimicrobial activity of sapienic acid. Our analyses indicated that sapienic acid treatment induces a unique stress response in P. gingivalis resulting in differential expression of proteins involved in a variety of metabolic pathways. This network of differentially regulated proteins was enriched in protein-protein interactions (P ‫؍‬ 2.98 ؋ 10 ؊8 ), including six KEGG pathways (P value ranges, 2.30 ؋ 10 ؊5 to 0.05) and four Gene Ontology (GO) molecular functions (P value ranges, 0.02 to 0.04), with multiple suggestive enriched relationships in KEGG pathways and GO molecular functions. Upregulated metabolic pathways suggest increases in energy production, lipid metabolism, iron acquisition and processing, and respiration. Combined with a suggested preferential metabolism of serine, which is necessary for fatty acid biosynthesis, these data support our previous findings that the site of sapienic acid antimicrobial activity is likely at the bacterial membrane. IMPORTANCE P. gingivalis is an important opportunistic pathogen implicated in periodontitis.Affecting nearly 50% of the population, periodontitis is treatable, but the resulting damage is irreversible and eventually progresses to tooth loss. There is a great need for natural products that can be used to treat and/or prevent the overgrowth of periodontal pathogens and increase oral health. Sapienic acid is endogenous to the oral cavity and is a potent antimicrobial agent, suggesting a potential therapeutic or prophylactic use for this fatty acid. This study examines the effects of sapienic acid treatment on P. gingivalis and highlights the membrane as the likely site of antimicrobial activity. Inflammation in the oral cavity ranges from mild and reversible inflammation of the gingiva (gingivitis) to a more chronic inflammation and destruction of tooth-supporting structures (periodontitis). Gingivitis occurs in 50 to 90% of adults worldwide, depending on the widely differing definitions of gingivitis (1). Periodontitis occurs in just over 47% of the U.S. population, with prevalences of 8.7, 30.0, and 8.5% for mild, moderate, and severe periodontitis depending on multiple factors, including oral hygiene, socioeconomic status, educational status, age, and other environmental, genetic, and metabolic risk factors (2). These numbers differ in different populations, and older individuals are at a particular...

show abstract

“…P. gingivalis fimbriae are associated with biofilm formation [37] and binding to other bacteria [38]. Production of fimbriae is regulated by the FimS-FimR TCS encoded by PGN_0904-PGN_0903 in strain ATCC 33277, and PG 1432-PG1431 in strain W83.…”

Section: P Gingivalis Fimsr Tcsmentioning

confidence: 99%

Two-component signal transduction systems in oral bacteria

Mattos-Graner

Duncan²

2017

Journal of Oral Microbiology

View full text Add to dashboard Cite

We present an overview of how members of the oral microbiota respond to their environment by regulating gene expression through two-component signal transduction systems (TCSs) to support conditions compatible with homeostasis in oral biofilms or drive the equilibrium toward dysbiosis in response to environmental changes. Using studies on the sub-gingival Gram-negative anaerobe Porphyromonas gingivalis and Gram-positive streptococci as examples, we focus on the molecular mechanisms involved in activation of TCS and species specificities of TCS regulons.

show abstract

Identification and Characterization of Porphyromonas gingivalis Client Proteins That Bind to Streptococcus oralis Glyceraldehyde-3-Phosphate Dehydrogenase

Cited by 24 publications

References 57 publications

Genome-Wide Identification of Genes Necessary for Biofilm Formation by Nosocomial Pathogen Stenotrophomonas maltophilia Reveals that Orphan Response Regulator FsnR Is a Critical Modulator

Genome-Wide Identification of Genes Necessary for Biofilm Formation by Nosocomial Pathogen Stenotrophomonas maltophilia Reveals that Orphan Response Regulator FsnR Is a Critical Modulator

Protein Analysis of Sapienic Acid-Treated Porphyromonas gingivalis Suggests Differential Regulation of Multiple Metabolic Pathways

Two-component signal transduction systems in oral bacteria

Contact Info

Product

Resources

About