Identification and characterization of receptors for granulocyte colony-stimulating factor on human placenta and trophoblastic cells.

Abstract

Since radioiodination of human granulocyte colony-stimulating factor (G-CSF) is difficult, we synthesized a mutein of human G-CSF that retains full biological activity and receptor-binding capacity for at least 2 weeks after radioiodination. Receptors for human G-CSF were characterized in the plasma membrane fraction from the human term placenta (human placental membranes) and trophoblastic cells by using the III-labeled mutein of human G-CSF (KW-2228). The specific binding of III-labeled KW-2228 to placental membranes was pH-dependent, with maximal specific binding at pH 7.8; it increased linearly with protein to 3.7 mg of protein per ml and was both time-and temperature-dependent, with maximal binding at 4°C after a 24-hr incubation. When we examined the ability of hematopoietic growth factors to inhibit 2-5I-labeled KW-2228 binding, we found that KW-2228 and intact human G-CSF inhibited '25I-labeled KW-2228 binding, whereas erythropoietin or granulocyte-macrophage colonystimulating factor did not. Scatchard analysis revealed a single receptor type with a Bn,,., of 210 fmol/mg of protein and a Kd of 480 pM. The human G-CSF receptors on human placental membranes were shown to consist of two molecular species of 150 kDa and 120 kDa that could be specifically cross-linked to

Granulocyte colony-stimulating factor receptor expression on human transitional cell carcinoma of the bladder

“…It should be pointed out, on the other hand, that G-CSF receptor expressions have been found on the surface of several nonhaematopoietic cell types, including human carcinoma cells (Bussolino et al, 1989;Uzumaki et al, 1989;Avaros et al, 1990). In fact, we previously reported that human transitional carcinoma cells expressing G-CSF receptor generated an acceleration of tumour cell proliferation (Ohigashi et al, 1992;Tachibana et al, 1995).…”

“…Controversy exists concerning whether or not these G-CSF receptors are similar to the receptors expressed on cells of neutrophilic lineage, binding G-CSF with high affinity, and whether they are present on the surface of these non-haematopoietic cell types in sparse numbers (Bussolino et al, 1989;Uzumaki et al, 1989;Avaros et al, 1990). Moreover, at present, five different human G-CSF receptor isoforms or classes arising from alternative RNA splicing have been isolated, which are all identical in the extracellular domain but differ in their downstream sequences (Avalos et al, 1996).…”

“…Meanwhile, G-CSF receptors have been noted to be present on such cells as myeloblasts and mature neutrophils (Nicola et al, 1985). The receptors for G-CSF have also been demonstrated and studied on a variety of other cells, including human myeloid leukaemic cells (Begley et al, 1987) and leukaemic cell lines (Park et al, 1989), human placenta and trophoblastic cells (Uzumaki et al, 1989), human vascular endothelial cells (Bussolino et al, 1989) and cell lines derived from human small-cell carcinoma of the lung (Avalos et al, 1990) and the bladder (Tachibana et al, 1995).…”

Granulocyte Colony Stimulating Factor for Treatment of Thin Endometrium in Assisted Reproduction Technology Cycles

“…15,16 The G-CSF has been found to beneficial effect on placental metabolism and trophoblast development. 17 Numerous cells involved in the reproductive physiological function, such as endothelium, fibroblasts, monocytes and endometrial cells produce G-CSF.…”

Placental therapy: An insight to their biological and therapeutic properties

“…Regulates cell cycle activation, survival of the myeloid lineage, mediates the proliferation, differentiation and survival of neutrophil progenitors [52,57] Granulocyte-macrophage colony stimulating factor (GM-CSF)…”