Identification and Characterization of Ribosomal Protein S8 Gene of Jute

Abstract

Abstract:Confirmation of ESTs (expressed sequence tags) from genomic clones is foremost in the identification of genes. To reveal the full length sequence of genes from ESTs and then to characterize the same without any, even presumptive information about the genome is difficult and tedious. But a combination of computational and experimental approaches is very useful in this regard. Jute has very little information at the genome level in the database. In this backdrop the present study was undertaken to identify jute EST(s) from a jute genomic library using computational approaches like ab initio and homology based searches together with common laboratory approaches. The study has led to the determination of the full length sequence of a jute gene (for the ribosomal protein, S8) using degenerate primer based gene walking from an identified jute EST. The sequence of this gene was found to be similar to ribosomal protein S8 gene of Hibiscus macrophyllus, Gossypium hirsutum, Carica papaya, Arabidopsis thaliana and a number of other plant species. This gene was further characterized for determining its cellular location. Key words: Bioinformatics, RT-PCR, Sequencing, Cellular localizationIntroduction: