A putative leucine-rich repeat receptor-like protein kinase (LRR-RLK) gene together with its 5′ and 3′ untranslated regions of jute (Corchorus olitorius L.) has been identified and sequenced. The gene is 3,371 bp long containing two exons and one intron. The coding sequence of the gene is 2,879 bp long encoding a peptide of 957 amino acids. The predicted protein contains several domains and motifs characteristic of a transmembrane protein kinase. It is complete with domains for an Nterminal leucine-rich repeat and a protein kinase core, an active site for serine/threonine protein kinase, an ATP binding conserved site and a transmembrane region.

Various reduction and fixation techniques have been introduced for the treatment of different types of fractures. Therefore, this study was planned to evaluate two methods of fracture treatments in calves having complete fractures due to automobile accident. In addition, a retrospective study was done to observe the occurrence of fractures in calves. Calves presented with the history of automobile accident and clinical signs suggestive of fractures were subjected to detailed physical, orthopaedic and radiographic examination to confirm fractures. For treatment 20 fractures case were included, where 8 cases were treated with closed reduction with external fixation using bamboo splints and plaster of paris. Whereas, open reduction with internal fixation using stainless steel orthopaedic wires and modified thomas splints were used to treat 12 fractures in long bones of calves aged 8 days to 5 months.All calves treated with open reduction and internal fixation showed good weight bearing in the immediate post-operative period. The fixators applied to different bones were well-tolerated, and the animals could lie down, stand and walk freely with the fixator without any problems after 60 days. Post operative radiography confirmed that bone fragments were well maintained until healing occurred and radiograph showed negligible callus and a radioluscent line (less remarkeble radiolucent area) at 60 days. There was no periosteal reaction around the wire suture at the fracture site. So, wire suture provided good alignment and stabilization of fracture fragments, rapid union of fracture resulting in early functional usage of limb. In contrast, two calves cured among 8 calves treated with closed reduction and external fixation, this study resulted that prognosis was not favorable in calves The results of this study exemplify the successful use of wire sutures in the treatment of complete fractures of the long bones in calves.

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Abstract:Confirmation of ESTs (expressed sequence tags) from genomic clones is foremost in the identification of genes. To reveal the full length sequence of genes from ESTs and then to characterize the same without any, even presumptive information about the genome is difficult and tedious. But a combination of computational and experimental approaches is very useful in this regard. Jute has very little information at the genome level in the database. In this backdrop the present study was undertaken to identify jute EST(s) from a jute genomic library using computational approaches like ab initio and homology based searches together with common laboratory approaches. The study has led to the determination of the full length sequence of a jute gene (for the ribosomal protein, S8) using degenerate primer based gene walking from an identified jute EST. The sequence of this gene was found to be similar to ribosomal protein S8 gene of Hibiscus macrophyllus, Gossypium hirsutum, Carica papaya, Arabidopsis thaliana and a number of other plant species. This gene was further characterized for determining its cellular location. Key words: Bioinformatics, RT-PCR, Sequencing, Cellular localizationIntroduction: