Identification and Characterization of Short Crown Root 8, a Temperature-Sensitive Mutant Associated with Crown Root Development in Rice

Hu, Peng; Wen, Yi; Wang, Yueying; Wu, Hao; Wang, Junge; Wu, Kaixiong; Chai, Bingze; LiXin, Zhu; Zhang, Guangheng; Gao, Zhenyu; Ren, Deyong; Zhu, Lu; Guo, Longbiao; Xu, Jing; Yan, Song; Qian, Qian; Rao, Yuchun; Hu, Jiang

doi:10.3390/ijms22189868

Cited by 5 publications

(3 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total RNA was isolated from various tissues and then complementary DNA (cDNA) syntheses were performed according to previously described method [ 40 ]. The cDNA was used as template for reverse transcription quantitative PCR (RT-qPCR) analysis by SYBR Green Real-time PCR Master Mix (Toyobo, Japan) in Applied Biosystems 7900HT Fast Real-Time PCR System.…”

Section: Methodsmentioning

confidence: 99%

NLG1, encoding a mitochondrial membrane protein, controls leaf and grain development in rice

Wen,

Wu,

Chai

et al. 2023

BMC Plant Biol

Self Cite

View full text Add to dashboard Cite

Background Mitochondrion is the key respiratory organ and participate in multiple anabolism and catabolism pathways in eukaryote. However, the underlying mechanism of how mitochondrial membrane proteins regulate leaf and grain development remains to be further elucidated. Results Here, a mitochondria-defective mutant narrow leaf and slender grain 1 (nlg1) was identified from an EMS-treated mutant population, which exhibits narrow leaves and slender grains. Moreover, nlg1 also presents abnormal mitochondria structure and was sensitive to the inhibitors of mitochondrial electron transport chain. Map-based cloning and transgenic functional confirmation revealed that NLG1 encodes a mitochondrial import inner membrane translocase containing a subunit Tim21. GUS staining assay and RT-qPCR suggested that NLG1 was mainly expressed in leaves and panicles. The expression level of respiratory function and auxin response related genes were significantly down-regulated in nlg1, which may be responsible for the declination of ATP production and auxin content. Conclusions These results suggested that NLG1 plays an important role in the regulation of leaf and grain size development by maintaining mitochondrial homeostasis. Our finding provides a novel insight into the effects of mitochondria development on leaf and grain morphogenesis in rice.

show abstract

Section: Methodsmentioning

confidence: 99%

NLG1, encoding a mitochondrial membrane protein, controls leaf and grain development in rice

Wen,

Wu,

Chai

et al. 2023

BMC Plant Biol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Total RNAs were isolated from various tissues and then complementary DNA (cDNA) syntheses were performed according to previously described method [35]. The cDNA was used as template for reverse transcription quantitative PCR (RT-qPCR) analysis by SYBR Green Real-time PCR Master Mix (Toyobo, Japan) in Applied Biosystems 7900HT Fast Real-Time PCR System.…”

Section: Rna Extraction and Rt-qpcrmentioning

confidence: 99%

NLG1, a novel mitochondrial membrane protein, control leaf and grain development in rice

Wen

Chai

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Background Mitochondrion is the key respiratory organ and participates in various anabolic and catabolic metabolic pathways in eukaryote. However, the underlying mechanism of how mitochondrial membrane proteins regulate leaf and grain development remains to be further elucidated. Results Here, a mitochondria-defective mutant narrow leaf and slender grain 1 (nlg1) was identified from an EMS-treated mutant population, which exhibits narrow leaves and slender grains. Map-based cloning and transgenic functional confirmation revealed that NLG1encodes a mitochondrial import inner membrane translocase containing a subunit Tim21 domain. NLG1 was identified as a mitochondria-localized translocase protein, which is mainly transcribed in leaves and panicles. Further analysis showed that the expression level of respiratory function and auxin response related genes were significantly down-regulated, resulting in ATP production and auxin content declined remarkably in nlg1. Moreover, nlg1 also exhibited abnormal mitochondria structure and was sensitive to the inhibitors of mitochondrial electron transport chain. Conclusions These results suggested that NLG1 plays an important role in the regulation of leaf and grain size development by maintaining mitochondrial homeostasis. Our finding provides a novel insight for exploring the relationship between mitochondria development and plant growth in rice.

show abstract

“…Hu et al [ 9 ] isolated and characterized a temperature-sensitive mutant which inhibits crown root development in rice at high temperatures. Crown roots help in absorbing water and nutrients, and any abnormality may impair plant growth and development.…”

Section: Introductionmentioning

confidence: 99%

Molecular Research in Rice

Subudhi

2023

IJMS

View full text Add to dashboard Cite

show abstract

Identification and Characterization of Short Crown Root 8, a Temperature-Sensitive Mutant Associated with Crown Root Development in Rice

Cited by 5 publications

References 66 publications

NLG1, encoding a mitochondrial membrane protein, controls leaf and grain development in rice

NLG1, encoding a mitochondrial membrane protein, controls leaf and grain development in rice

NLG1, a novel mitochondrial membrane protein, control leaf and grain development in rice

Molecular Research in Rice

Contact Info

Product

Resources

About