Identification and characterization of simple sequence repeat markers from a glandular <i>Origanum vulgare</i> expressed sequence tag

Novak, Johannes; Lukas, Brigitte; Bolzer, Kerstin; Grausgruber-Gröger, Sabine; Degenhardt, Jörg

doi:10.1111/j.1471-8286.2007.02059.x

Cited by 36 publications

(32 citation statements)

References 10 publications

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“…A prerequisite for all of these strategies is the development of a transformation system for O. vulgare. However, in the meantime, the terpene synthase genes can still be utilized as markers for directed breeding of O. vulgare varieties with higher value essential oil (Novak et al 2008).…”

Section: Terpene Synthases Have Value For Molecular Engineering Of Tementioning

confidence: 99%

Terpene synthases of oregano (Origanum vulgare L.) and their roles in the pathway and regulation of terpene biosynthesis

et al. 2010

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The aroma, flavor and pharmaceutical value of cultivated oregano (Origanum vulgare L.) is a consequence of its essential oil which consists mostly of monoterpenes and sesquiterpenes. To investigate the biosynthetic pathway to oregano terpenes and its regulation, we identified and characterized seven terpene synthases, key enzymes of terpene biosynthesis, from two cultivars of O. vulgare. Heterologous expression of these enzymes showed that each forms multiple mono- or sesquiterpene products and together they are responsible for the direct production of almost all terpenes found in O. vulgare essential oil. The correlation of essential oil composition with relative and absolute terpene synthase transcript concentrations in different lines of O. vulgare demonstrated that monoterpene synthase activity is predominantly regulated on the level of transcription and that the phenolic monoterpene alcohol thymol is derived from gamma-terpinene, a product of a single monoterpene synthase. The combination of heterologously-expressed terpene synthases for in vitro assays resulted in blends of mono- and sesquiterpene products that strongly resemble those found in vivo, indicating that terpene synthase expression levels directly control the composition of the essential oil. These results will facilitate metabolic engineering and directed breeding of O. vulgare cultivars with higher quantity of essential oil and improved oil composition.

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Section: Terpene Synthases Have Value For Molecular Engineering Of Tementioning

confidence: 99%

Terpene synthases of oregano (Origanum vulgare L.) and their roles in the pathway and regulation of terpene biosynthesis

et al. 2010

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“…The chromosome number of o. vulgare was previously reported to be 2n = 2x = 32 [6]. Identification of SSRs (Simple Sequence Repeats) derived from ESTs (Expressed Sequence Tags) of epidermal glands on this commercial subspecies was also reported [7]. In a previous work, we have investigated genetic relationships between different populations of o. vulgare using two PCR-based marker approaches, Amplified Fragment Length Polymorphism (AFLP) and Selectively Amplified Microsatellite Polymorphic Loci (SAMPL), and we have also compared the relative efficiencies of these two marker systems for surveying intraspecific genetic diversity [8].…”

Section: Introductionmentioning

confidence: 97%

Statistical analysis of the associations between phenolic monoterpenes and molecular markers, AFLPs and SAMPLs in the spice plant Oregano

2016

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S u m m a r yIntroduction: Molecular markers are the examples of the contribution of genome technology to medicinal plant breeding through marker-assisted selection (MAS) for pharmaceutical quality. Objective: Forty-two accessions of origanum vulgare L. originating from Europe were evaluated to detect genomic and chemotypic polymorphisms and to discover possible associations between them. Methods: A total of 477 molecular polymorphisms including 214 AFLP (Amplified Fragment Length Polymorphism) and 263 SAMPL (Selectively Amplified Microsatellite Polymorphic Loci) were used for genotyping. Components in the essential oils were identified and quantified by gas chromatography (GC) and two major compounds (two economically important monoterpenes: carvacrol and thymol) were investigated. Results: Based on results, a relatively high correlation between chemotypic patterns and genetic markers was identified. essential oils (carvacrol and thymol content) and genetic markers were tested using five statistical methods including three General Linear Model (GLM) and two unified Mixed Linear Model (MLM) approaches. Significant associations were found for 3 AFLP and 20 SAMPL with three key traits including essential oil yield, carvacrol and thymol content. Conclusion: These associations can constitute a useful starting point for marker-assisted selection. Therefore, the results provide the basis for molecular breeding of o. vulgare for pharmaceutical purposes.

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“…Due to the production of aromatic oils and secondary metabolites, some of these plants are commonly used as spices for cooking such as basil, marjoram, mint, oregano, rosemary, sage, savory and thyme. In this context, many members of the Lamiaceae are characterized by considerable commercial importance and have been previously investigated both at the species and cultivar levels in order to characterize their variability and properties (Labra et al, 2004;Novak, Lukas, Bolzer, Grausgruber-Gröger, & Degenhardt, 2008;Trindade, 2010). Systematic and phylogenetic studies on aromatic plants were usually based on morphological characters (Gurcharan, 2004).…”

Section: Introductionmentioning

confidence: 99%

A comparative study of different DNA barcoding markers for the identification of some members of Lamiacaea

Mattia

Bruni

Galimberti

et al. 2011

Food Research International

137

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Identification and characterization of simple sequence repeat markers from a glandular Origanum vulgare expressed sequence tag

Cited by 36 publications

References 10 publications

Terpene synthases of oregano (Origanum vulgare L.) and their roles in the pathway and regulation of terpene biosynthesis

Terpene synthases of oregano (Origanum vulgare L.) and their roles in the pathway and regulation of terpene biosynthesis

Statistical analysis of the associations between phenolic monoterpenes and molecular markers, AFLPs and SAMPLs in the spice plant Oregano

A comparative study of different DNA barcoding markers for the identification of some members of Lamiacaea

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