1997
DOI: 10.1128/jb.179.9.2892-2899.1997
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Identification and characterization of ssb and uup mutants with increased frequency of precise excision of transposon Tn10 derivatives: nucleotide sequence of uup in Escherichia coli

Abstract: A Lac؉ papillation assay was used to identify mutants (tex) of Escherichia coli that exhibit an increased frequency of precise excision of a lacZ::Tn10dKan insertion. Three tex strains had suffered mutations in the gene (ssb) encoding the essential single-stranded DNA-binding protein SSB, which resulted in the following alterations in the 177-residue protein: G4D; L10F, P24S; and V102M. The phenotypes of these ssb mutants indicated that they were largely unaffected in other functions mediated by SSB, such as D… Show more

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Cited by 30 publications
(35 citation statements)
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“…Mutations that elevate the precise and nearly precise excision of the transposon Tn10 or Tn5 at short direct repeats within or flanking the element (29) have defined the so-called tex mutants (27,28). Mutations in the uup gene also exhibit a Tex phenotype (30)(31)(32). The function of Uup is not known but it appears to be a general suppressor of RecAindependent rearrangements because RecA-independent tandem repeat deletion (unassociated with transposons) is also enhanced in uup mutants (32).…”
Section: Resultsmentioning
confidence: 99%
“…Mutations that elevate the precise and nearly precise excision of the transposon Tn10 or Tn5 at short direct repeats within or flanking the element (29) have defined the so-called tex mutants (27,28). Mutations in the uup gene also exhibit a Tex phenotype (30)(31)(32). The function of Uup is not known but it appears to be a general suppressor of RecAindependent rearrangements because RecA-independent tandem repeat deletion (unassociated with transposons) is also enhanced in uup mutants (32).…”
Section: Resultsmentioning
confidence: 99%
“…To study the ability of uup and its Walker motif B variants to complement a null mutant of uup, these genes were placed under the control of the araBAD promoter into vector pBAD18 (24), and these constructs were introduced into the prophage attB site of MG1655 by using the InCh system (25). The attB-inserted genes, whose expression was inducible by L-arabinose at a final concentration of 0.2%, were transduced in strain GJ1886, which carried a uup gene inactivated by the insertion of a defective Tn10 transposon (7). Then the araBAD::aadA7 mutation was transduced from MG1655⌬araBAD⌬lacIZ into these strains to avoid the effects of arabinose utilization on the phenotype of mutants on MacConkey lactose plates.…”
Section: Purification Of His 6 -Uup and Itsmentioning
confidence: 99%
“…The uup variants carrying mutations of Asp-181 (uup1) or Asp-465 (uup2) to asparagine and the double mutant (uup3) were studied in vivo with regard to function in a colony papillation assay (7). In this assay, GJ1885 cells harboring a wild-type uup gene display one or two papilla in 50% of the colonies, whereas 100% colonies of GJ1886 cells carrying a uup null mutation show about 20 red papilla (Fig.…”
Section: Mg-atp-induced Conformational Changes As Assessed By Limitedmentioning
confidence: 99%
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