Identification and Characterization of Terpene Synthases Potentially Involved in the Formation of Volatile Terpenes in Carrot (<i>Daucus carota</i> L.) Roots

Yahyaa, Mosaab; Tholl, Dorothea; Cormier, G.; Jensen, Roderick V.; Simon, Philipp W.; Ibdah, Mwafaq

doi:10.1021/acs.jafc.5b00546

Cited by 65 publications

(52 citation statements)

References 50 publications

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“…In angiosperms, the TPS-b and TPS-a subfamilies account for most of the sesquiterpene synthases and monoterpene synthases, which constitute the majority of TPS proteins. DCAR_023152 (DcTPS1) and DCAR_012963 (DcTPS2) were two TPSs found in carrot belonging to the TPS-a subfamily and TPS-b subfamily, respectively 67 . The TPSs of celery showed the closest phylogenetic relationships with those of carrot, another Apiaceae family plant.…”

Section: Terpenoid Synthase Family Genesmentioning

confidence: 99%

The genome sequence of celery (Apium graveolens L.), an important leaf vegetable crop rich in apigenin in the Apiaceae family

Li¹,

Feng²,

Hou³

et al. 2020

Hortic Res

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Celery (Apium graveolens L.) is a vegetable crop in the Apiaceae family that is widely cultivated and consumed because it contains necessary nutrients and multiple biologically active ingredients, such as apigenin and terpenoids. Here, we report the genome sequence of celery based on the use of HiSeq 2000 sequencing technology to obtain 600.8 Gb of data, achieving~189-fold genome coverage, from 68 sequencing libraries with different insert sizes ranging from 180 bp to 10 kb in length. The assembled genome has a total sequence length of 2.21 Gb and consists of 34,277 predicted genes. Repetitive DNA sequences represent 68.88% of the genome sequences, and LTR retrotransposons are the main components of the repetitive sequences. Evolutionary analysis showed that a recent whole-genome duplication event may have occurred in celery, which could have contributed to its large genome size. The genome sequence of celery allowed us to identify agronomically important genes involved in disease resistance, flavonoid biosynthesis, terpenoid metabolism, and other important cellular processes. The comparative analysis of apigenin biosynthesis genes among species might explain the high apigenin content of celery. The whole-genome sequences of celery have been deposited at CeleryDB (http://apiaceae.njau.edu.cn/celerydb). The availability of the celery genome data advances our knowledge of the genetic evolution of celery and will contribute to further biological research and breeding in celery as well as other Apiaceae plants.

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Section: Terpenoid Synthase Family Genesmentioning

confidence: 99%

The genome sequence of celery (Apium graveolens L.), an important leaf vegetable crop rich in apigenin in the Apiaceae family

Li¹,

Feng²,

Hou³

et al. 2020

Hortic Res

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“…Cells were further grown for 12 h at 18°C. Bacteria were lysed and soluble protein purified by nickel chromatography and assayed for purity by SDS-PAGE as previously described by Yahyaa et al (2015). An optimized LnTPS3 ORF for expression in E. coli was synthesized and cloned into pUC57 vector (GenScript).…”

Section: Preparation Of Bacterial Lysatesmentioning

confidence: 99%

Identification, Functional Characterization, and Evolution of Terpene Synthases from a Basal Dicot

Yahyaa¹,

Matsuba²,

Brandt³

et al. 2015

Plant Physiol.

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“…High ethanol emissions (1 mg kg -1 ) from carrots and acetaldehyde emissions from carrots (0.5 mg kg -1 ) and zucchini (0.3 mg kg -1 ) could originate from drought stress conditions during the growth, mechanical stress after the harvest or storage under low oxygen conditions 39. Boiling carrots additionally emits considerable amounts of sesquiterpenes (0.3 mg kg -1 ) which are formed during root development in the plants 40.…”

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confidence: 99%

Characterization of Gas-Phase Organics Using Proton Transfer Reaction Time-of-Flight Mass Spectrometry: Cooking Emissions

Klein

Platt

Farren

et al. 2016

Environ. Sci. Technol.

110

125

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Cooking processes produce gaseous and particle emissions that are potentially deleterious to human health. Using a highly controlled experimental setup involving a proton-transfer-reaction time-of-flight mass spectrometer (PTR-ToF-MS), we investigate the emission factors and the detailed chemical composition of gas phase emissions from a broad variety of cooking styles and techniques. A total of 95 experiments were conducted to characterize nonmethane organic gas (NMOG) emissions from boiling, charbroiling, shallow frying, and deep frying of various vegetables and meats, as well as emissions from vegetable oils heated to different temperatures. Emissions from boiling vegetables are dominated by methanol. Significant amounts of dimethyl sulfide are emitted from cruciferous vegetables. Emissions from shallow frying, deep frying and charbroiling are dominated by aldehydes of differing relative composition depending on the oil used. We show that the emission factors of some aldehydes are particularly large which may result in considerable negative impacts on human health in indoor environments. The suitability of some of the aldehydes as tracers for the identification of cooking emissions in ambient air is discussed.

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Identification and Characterization of Terpene Synthases Potentially Involved in the Formation of Volatile Terpenes in Carrot (Daucus carota L.) Roots

Cited by 65 publications

References 50 publications

The genome sequence of celery (Apium graveolens L.), an important leaf vegetable crop rich in apigenin in the Apiaceae family

The genome sequence of celery (Apium graveolens L.), an important leaf vegetable crop rich in apigenin in the Apiaceae family

Identification, Functional Characterization, and Evolution of Terpene Synthases from a Basal Dicot

Characterization of Gas-Phase Organics Using Proton Transfer Reaction Time-of-Flight Mass Spectrometry: Cooking Emissions

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