Identification and Characterization of the Intra-Articular Microbiome in the Osteoarthritic Knee

Tsai, Joseph C.; Casteneda, Grant; Lee, Abby; Dereschuk, Kypros; Li, Wei Tse; Chakladar, Jaideep; Lombardi, Alecio; Ongkeko, Weg M.; Chang, Eric Y.

doi:10.3390/ijms21228618

Cited by 21 publications

(17 citation statements)

References 61 publications

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“…Of the 43 differentially abundant microbes, 27 of them were species of Pseudomonas . The bacterium found in OA synovial biopsies can be linked to immune signatures and immune cell types ( Tsai et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

“…The 8 overlapping DEGs that were found have a pivotal role in modulating inflammation and degradation of cartilage, and more so the inflammatory pathway was discussed to be differently expressed in all 5 articles chosen for this review. Interestingly, the differentially abundant bacteria found by Tsai et al were majority from the species Pseudomonas ( Tsai et al, 2020 ). There were also E. Coli differentially expressed.…”

Section: Discussionmentioning

confidence: 96%

“…It has been shown that gut permeability is increased in obese and aging populations, which are both predisposed to OA ( Nagpal et al, 2018 ; Portincasa et al, 2021 ). There is a possible linkage between pathogenic bacteria leaking from the gut migrates into the synovium to create systemic inflammation leading to the onset of OA ( Collins et al, 2015 ; Ulici et al, 2018 ; Tsai et al, 2020 ). The 8 overlapping DEGs that were found have a pivotal role in modulating inflammation and degradation of cartilage, and more so the inflammatory pathway was discussed to be differently expressed in all 5 articles chosen for this review.…”

Section: Discussionmentioning

confidence: 99%

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RNAseq of Osteoarthritic Synovial Tissues: Systematic Literary Review

2022

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Osteoarthritis (OA) is one of the most common causes of disability in aged people, and it is defined as a degenerative arthropathy, characterized by the disruption in joint tissue. The synovium plays a vital role in maintaining the health of the joint by supplying the nutrients to the surrounding tissues and the lubrication for joint movement. While it is well known that all the joint tissues are communicating and working together to provide a functioning joint, most studies on OA have been focused on bone and cartilage but much less about synovium have been reported. The purpose of this review was to investigate the current literature focused on RNA sequencing (RNAseq) of osteoarthritic synovial tissues to further understand the dynamic transcriptome changes occurring in this pivotal joint tissue. A total of 3 electronic databases (PubMed, CINHAL Complete, and Academic Complete) were systematically searched following PRISMA guidelines. The following criteria was used for inclusion: English language, free full text, between the period 2011–2022, size of sample (n > 10), study design being either retrospective or prospective, and RNAseq data of synovial tissue from OA subjects. From the initial search, 174 articles, 5 met all of our criteria and were selected for this review. The RNAseq analysis revealed several differentially expressed genes (DEGs) in synovial tissue. These genes are related to the inflammatory pathway and regulation of the extracellular matrix. The MMP family, particularly MMP13 was identified by three of the studies, indicating its important role in OA. IL6, a key contributor in the inflammation pathway, was also identified in 3 studies. There was a total of 8 DEGs, MMP13, MMP1, MMP2, APOD, IL6, TNFAIP6, FCER1G, and IGF1 that overlapped in 4 out of the 5 studies. One study focused on microbial RNA in the synovial tissue found that the microbes were differentially expressed in OA subjects too. These differentially expressed microbes have also been linked to the inflammatory pathway. Further investigation with more clinical gene profiling in synovial tissue of OA subjects is required to reveal the causation and progression, as well as aid in the development of new treatments.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

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RNAseq of Osteoarthritic Synovial Tissues: Systematic Literary Review

2022

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“…It has been shown that bacteria or related compounds (i.e., LPS and peptidoglycan) cross the intestinal barrier and enter the systemic circulation to mediate OA ( Lorenzo et al., 2019 ). Based on the simultaneous assessment of the microbiome in the gut, blood and joints, Tsai et al. (2020) also made the hypothesis that a “leaky” gut allows microbiota, associated with dysregulation of immune gene signaling and promoting inflammation, to migrate from the gut to the joints, leading to the onset of OA.…”

Section: Gut Microbiota Is Involved In the Development Of Oa Through ...mentioning

confidence: 99%

Association Between Gut Microbiota and Osteoarthritis: A Review of Evidence for Potential Mechanisms and Therapeutics

Wei

2022

Front. Cell. Infect. Microbiol.

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Osteoarthritis (OA) is a multifactorial joint disease characterized by degeneration of articular cartilage, which leads to joints pain, disability and reduced quality of life in patients with OA. Interpreting the potential mechanisms underlying OA pathogenesis is crucial to the development of new disease modifying treatments. Although multiple factors contribute to the initiation and progression of OA, gut microbiota has gradually been regarded as an important pathogenic factor in the development of OA. Gut microbiota can be regarded as a multifunctional “organ”, closely related to a series of immune, metabolic and neurological functions. This review summarized research evidences supporting the correlation between gut microbiota and OA, and interpreted the potential mechanisms underlying the correlation from four aspects: immune system, metabolism, gut-brain axis and gut microbiota modulation. Future research should focus on whether there are specific gut microbiota composition or even specific pathogens and the corresponding signaling pathways that contribute to the initiation and progression of OA, and validate the potential of targeting gut microbiota for the treatment of patients with OA.

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“…It has been stated that old age may be associated with intestinal dysbiosis and increased gut permeability, which could promote a “leakage” of gut microbes into tissues, thus leading to a chronic proinflammatory state [ 173 , 174 ]. Tsai et al aimed to characterize the immunological signature of the microbiome in osteoarthritic knee synovium, finding marked discrepancy in microbial abundance in patients compared to healthy controls [ 175 ]. Whereas intestinal dysbiosis has been linked to synovial inflammation in knee OA, the impact of probiotic use on disease progression have not been studied extensively in clinical trials [ 176 , 177 ].…”

Section: Therapeutic Approach To Synovial Inflammation In Knee Ostmentioning

confidence: 99%

From Pathogenesis to Therapy in Knee Osteoarthritis: Bench-to-Bedside

Rezuş

Burlui

Cardoneanu

et al. 2021

IJMS

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Osteoarthritis (OA) is currently the most widespread musculoskeletal condition and primarily affects weight-bearing joints such as the knees and hips. Importantly, knee OA remains a multifactorial whole-joint disease, the appearance and progression of which involves the alteration of articular cartilage as well as the synovium, subchondral bone, ligaments, and muscles through intricate pathomechanisms. Whereas it was initially depicted as a predominantly aging-related and mechanically driven condition given its clear association with old age, high body mass index (BMI), and joint malalignment, more recent research identified and described a plethora of further factors contributing to knee OA pathogenesis. However, the pathogenic intricacies between the molecular pathways involved in OA prompted the study of certain drugs for more than one therapeutic target (amelioration of cartilage and bone changes, and synovial inflammation). Most clinical studies regarding knee OA focus mainly on improvement in pain and joint function and thus do not provide sufficient evidence on the possible disease-modifying properties of the tested drugs. Currently, there is an unmet need for further research regarding OA pathogenesis as well as the introduction and exhaustive testing of potential disease-modifying pharmacotherapies in order to structure an effective treatment plan for these patients.

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Identification and Characterization of the Intra-Articular Microbiome in the Osteoarthritic Knee

Cited by 21 publications

References 61 publications

RNAseq of Osteoarthritic Synovial Tissues: Systematic Literary Review

RNAseq of Osteoarthritic Synovial Tissues: Systematic Literary Review

Association Between Gut Microbiota and Osteoarthritis: A Review of Evidence for Potential Mechanisms and Therapeutics

From Pathogenesis to Therapy in Knee Osteoarthritis: Bench-to-Bedside

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