Identification and characterization of the first cytokinin glycosyltransferase from rice

Li, Pan; Lei, Kang; Li, Yanjie; He, Xingrui; Wang, Shuo; Liu, Renmin; Ji, Lusha; Hou, Bing-Kai

doi:10.1186/s12284-019-0279-9

Cited by 21 publications

(21 citation statements)

References 26 publications

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“…Kudo et al (2012) identified three putative cis-ZOGTs in rice, which were shown to preferentially O-glucosylate cZ and cZR rather than tZ and tZR; additionally, cZOGT1 and 2 showed some activity towards tZ, while cZOGT3 was active towards both tZ and tZR. Recently, Li et al (2019) showed a purified Os6 protein Nglucosylated cytokinin in vitro. Heterologous expression in arabidopsis confirmed 7-and 9-NGT activity in planta.…”

Section: Cytokinin Glucosylation Genesmentioning

confidence: 99%

Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement

Chen

Zhao

Song

et al. 2021

Plant Biotechnology Journal

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The cytokinins, which are N 6 -substituted adenine derivatives, control key aspects of crop productivity. Cytokinin levels are controlled via biosynthesis by isopentenyl transferase (IPT), destruction by cytokinin oxidase/dehydrogenase (CKX), and inactivation via glucosylation by cytokinin glucosyl transferases (CGTs). While both yield components and tolerance to drought and related abiotic stressors have been positively addressed via manipulation of IPT and/or CKX expression, much less attention has been paid to the CGTs. As naming of the CGTs has been unclear, we suggest COGT, CNGT, CONGT and CNOGT to describe the O-, N-and dual function CGTs. As specific CGT mutants of both rice and arabidopsis showed impacts on yield components, we interrogated the wheat genome database, IWGSC RefSeq v1.0 & v2.0, to investigate wheat CGTs. Besides providing unambiguous names for the 53 wheat CGTs, we show their expression patterns in 70 developmental tissues and their response characteristics to various stress conditions by reviewing more than 1000 RNA-seq data sets. These revealed various patterns of responses and showed expression generally being more limited in reproductive tissues than in vegetative tissues. Multiple cis-regulatory elements are present in the 3 kb upstream of the start codons of the 53 CGTs. Elements associated with abscisic acid, light and methyl jasmonate are particularly over-represented, indicative of the responsiveness of CGTs to the environment. These data sets indicate that CGTs have potential value for wheat improvement and that these could be targeted in TILLING or gene editing wheat breeding programmes.genes controlling the formation of the deactivated cytokinin Oand N-glucosides. After introducing the cytokinins more generally, this article focuses on the cytokinin O-and N-glucosyl transferases and their potential roles in modifying yield in bread wheat, under ambient and stress conditions. Cytokinin biosynthesis and metabolismNaturally occurring cytokinins, which are adenine derivatives, fall into two groups: those with an isoprenoid side chain and those with an aromatic one. The isoprenoid cytokinins are considered to be the predominant forms (Jaworek et al., 2019;Sakakibara, 2006). The aromatics are not covered in this review as, while not exhaustively looked for in all cereals, they were not detected in either maize leaves (Lacuesta et al., 2018) or in rice (Kudo et al., 2012), although low levels of 6-benzylamino purine were recently reported in wheat spikes (Jabło nski et al., 2020) and low levels of various aromatic metabolites have been detected in germinating seeds of maize and oats (Stirk et al., 2012).

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Section: Cytokinin Glucosylation Genesmentioning

confidence: 99%

Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement

Chen

Zhao

Song

et al. 2021

Plant Biotechnology Journal

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“…cazy.org). Family-1 glycosyltransferases are the largest multigene GT family, and their glycosylated products participate in the regulation of plant growth and development, such as hormone homeostasis 13 , pollination 14 , and interactions with the environment, such as detoxification of xenobiotics 15 as well as UV tolerance 16 . GT1s are also called UDP-glycosyltransferases because they use uridine 5'-diphosphate as a sugar donor.…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum

Ren

Jiao

et al. 2020

Hortic Res

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Flavonoids, which are a diverse class of phytonutrients, are used by organisms to respond to nearly all abiotic stresses and are beneficial for human health. Glycosyltransferase, used during the last step of flavonoid biosynthesis, is important in flavonoid enrichment. However, little is known about glycosyltransferase in the orchid Dendrobium catenatum (D. officinale). In this study, we isolated a novel C-glycosyltransferase (designated DcaCGT) from the orchid D. catenatum by identifying and analyzing 82 putative genes in the GT1 family. DcaCGT could specifically catalyze not only di-C-glycosylation but also O-glycosylation. Apart from the normal function of catalyzing 2-hydroxynaringenin and phloretin to the respective di-C-glycosides, DcaCGT also catalyzes apigenin to cosmosiin. Targeted metabolic profiling of the substrates (2-hydroxynaringenin, phloretin, and apigenin) and products (vitexin, isovitexin, vicenin-2, nothofagin, 3',5'-di-C-glucosylphloretin, and cosmosiin) in different tissues showed that vicenin-2 was the most abundant product of this novel enzyme. Cosmosiin was detected in flowers and flower buds. We also established that DcaCGT functions expanded throughout the evolution of D. catenatum. Residual OGT activity may help D. catenatum resist drought stress. Our study illustrates the function, origin, and differentiation of DcaCGT and provides insights into glycosylation and molecular propagation processes, which can be used to improve the production of flavonoids by the cultivated medicinal plant D. catenatum.

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“…For protein purification, the GST-tagged protein on the prokaryotic expression vector could be successfully eluted by binding to agarose beads Glutathione Sephrose 4B (purchased from General Electric Company). The specific method was performed in accordance with the work of Li et al (2019), after which the active enzyme was obtained.…”

Section: Methodsmentioning

confidence: 99%

“…For the in vitro enzymatic reaction, the reaction system is shown in Table S1. The specific operation steps were performed in accordance with the work of Li et al (2019).…”

Section: Methodsmentioning

confidence: 99%

Genome-wide analysis of the apple family 1 glycosyltransferases identified a flavonoid-modifying UGT, MdUGT83L3, which is targeted by MdMYB88 and contributes to stress adaptation

Zhang

et al. 2021

Preprint

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SummaryFlavonoids are a class of polyphenol compounds that are widespread in plants. They play an important role in plant growth and development. In this study, we found a mutant strain of M. baccata with yellow leaves (YL). Transcriptome sequencing revealed that it exhibited significant changes in the flavonoid metabolism pathway, which screening revealed was associated with a glycosyltransferase gene, MD09G1064900 (MdGT1). Analysis of its spatiotemporal expression showed that MdGT1 was mainly expressed in the stem and leaves, it means that MdGT1 may have a functional role in these parts. Real-time PCR and HPLC showed that MdGT1 was significantly upregulated by anthocyanin and exhibited strong anthocyaninase activity in vitro, respectively. An MdGT1 plant expression vector was constructed and overexpressed in apple fruit callus, resulting in a significant decrease of anthocyanin. Phenotypic observation also revealed that the MdGT1-overexpressing lines exhibited worse growth than the wild type after NaCl treatment, while they grew better upon the addition of exogenous anthocyanins. Moreover, real-time PCR and physiological data showed that MdGT1 is involved in salt stress and closely related to antioxidant pathways. Electrophoretic mobility shift assays (EMSA) and yeast one-hybrid experiments also proved that the transcription factor MdMYB88 is an upstream regulatory factor of MdGT1. The sequencing results revealed an amino acid insertion in an MdMYB88 HTH domain (between 77-131 amino acids) in the YL mutant strain. In conclusion, we identified a new apple glycosyltransferase gene, MdGT1, which may affect the color of apple leaves by glycosylating anthocyanins, and be regulated by the upstream transcription factor MdMYB88.Significance statementThe glycosyltransferases and their physiological significance in apple are largely unknown. Here we revealed that the MdMYB88-regulated apple glycosyltransferase gene MdGT1 plays a crucial role in the color of apple leaves and enhances plant tolerance to salt by antioxidant pathways via anthocyanin metabolism.

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Identification and characterization of the first cytokinin glycosyltransferase from rice

Cited by 21 publications

References 26 publications

Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement

Cytokinin glucosyl transferases, key regulators of cytokinin homeostasis, have potential value for wheat improvement

Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum

Genome-wide analysis of the apple family 1 glycosyltransferases identified a flavonoid-modifying UGT, MdUGT83L3, which is targeted by MdMYB88 and contributes to stress adaptation

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