Identification and characterization of two variants of the Hfq‐sRNA‐chaperone in the fish pathogen <i>Piscirickettsia salmonis</i>

Marshall, Sergio H.; Flores-Herrera, Patricio; Henríquez, F A; Gómez, Fernando

doi:10.1111/jfd.12752

Cited by 2 publications

(2 citation statements)

References 46 publications

(55 reference statements)

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“…mykiss ) [ 16 ]. Although this pathogen is present in other salmonid-producing countries, such as Norway [ 17 ], Canada [ 17 , 18 ], Scotland, Ireland [ 17 ], and Australia [ 19 ], this bacterium is much more aggressive in Chile due to certain genetic traits [ 15 , 20 ]. Although P .…”

Section: Introductionmentioning

confidence: 99%

Extended antibiotic treatment in salmon farms select multiresistant gut bacteria with a high prevalence of antibiotic resistance genes

et al. 2018

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The high use of antibiotics for the treatment of bacterial diseases is one of the main problems in the mass production of animal protein. Salmon farming in Chile is a clear example of the above statement, where more than 5,500 tonnes of antibiotics have been used over the last 10 years. This has caused a great impact both at the production level and on the environment; however, there are still few works in relation to it. In order to demonstrate the impact of the high use of antibiotics on fish gut microbiota, we have selected four salmon farms presenting a similar amount of fish of the Atlantic salmon species (Salmo salar), ranging from 4,500 to 6,000 tonnes. All of these farms used treatments with high doses of antibiotics. Thus, 15 healthy fish were selected and euthanised in order to isolate the bacteria resistant to the antibiotics oxytetracycline and florfenicol from the gut microbiota. In total, 47 bacterial isolates resistant to florfenicol and 44 resistant to oxytetracycline were isolated, among which isolates with Minimum Inhibitory Concentrations (MIC) exceeding 2048 μg/mL for florfenicol and 1024 μg/mL for oxytetracycline were found. In addition, another six different antibiotics were tested in order to demonstrate the multiresistance phenomenon. In this regard, six isolates of 91 showed elevated resistance values for the eight tested antibiotics, including florfenicol and oxytetracycline, were found. These bacteria were called “super-resistant” bacteria. This phenotypic resistance was verified at a genotypic level since most isolates showed antibiotic resistance genes (ARGs) to florfenicol and oxytetracycline. Specifically, 77% of antibiotic resistant bacteria showed at least one gene resistant to florfenicol and 89% showed at least one gene resistant to oxytetracycline. In the present study, it was demonstrated that the high use of the antibiotics florfenicol and oxytetracycline has, as a consequence, the selection of multiresistant bacteria in the gut microbiota of farmed fish of the Salmo salar species at the seawater stage. Also, the phenotypic resistance of these bacteria can be correlated with the presence of antibiotic resistance genes.

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Section: Introductionmentioning

confidence: 99%

Extended antibiotic treatment in salmon farms select multiresistant gut bacteria with a high prevalence of antibiotic resistance genes

et al. 2018

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“…Primers designed with Primer Premier 5.0 were listed in Supporting Information Table S2. 16S RNA was chosen for the expression levels normalizing, while mean expression levels were calculated with the 2 ‐ΔΔCt method ( n = 6) (Huang et al, ; Marshall, Flores‐Herrera, Henríquez, & Gómez, ).…”

Section: Methodsmentioning

confidence: 99%

A metabolomic investigation into the temperature‐dependent virulence of Pseudomonas plecoglossicida from large yellow croaker (Pseudosciaena crocea)

Huang

Zuo

Jiang

et al. 2019

Journal of Fish Diseases

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Pseudomonas plecoglossicida is associated with multiple fish diseases, and temperature is one of the most important environmental factors related to its outbreak. To elucidate the influence of temperature variation on the pathogen, the global metabolomics of P. plecoglossicida (NZBD9) were analysed at the virulent (18°C) and avirulent (12°C and 28°C) temperatures. The result showed that the levels of Phosphoric acid, Tyrosine, Spermidine and Sucrose were significantly reduced，while Itaconic acid, Glucaric acid and Isomaltose were increased in P. plecoglossicida at 18°C. These metabolic adjustments assist P. plecoglossicida to survive in adverse environments, proliferate in the host, colonize and resist host immune clearance during the initial steps of infection. The results suggested that L321_03626 and L321_18122 genes played a key role in the regulation of these metabolic adaptions and thus regulated P. plecoglossicida virulence at virulent temperature, which was proved by further gene silencing and artificial infection. The present study, for the first time, determines the P. plecoglossicida metabolomic responses to temperature variation, which is helpful to explore its pathogenic mechanism and provides reference for disease control.

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Identification and characterization of two variants of the Hfq‐sRNA‐chaperone in the fish pathogen Piscirickettsia salmonis

Cited by 2 publications

References 46 publications

Extended antibiotic treatment in salmon farms select multiresistant gut bacteria with a high prevalence of antibiotic resistance genes

Extended antibiotic treatment in salmon farms select multiresistant gut bacteria with a high prevalence of antibiotic resistance genes

A metabolomic investigation into the temperature‐dependent virulence of Pseudomonas plecoglossicida from large yellow croaker (Pseudosciaena crocea)

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