A metabolomic investigation into the temperature‐dependent virulence of <i>Pseudomonas plecoglossicida</i> from large yellow croaker (<i>Pseudosciaena crocea</i>)

Huang, Lixing; Zuo, Yanfei; Jiang, Qingling; Su, Yongquan; Qin, Yingxue; Xu, Xiaojin; Zhao, Lingmin; Yan, Qingpi

doi:10.1111/jfd.12957

Cited by 62 publications

(31 citation statements)

References 69 publications

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“…Short‐hairpin RNA (shRNA) sequences targeting the mRNA coding region of LuxR‐type regulators were synthesized by Shanghai Generay Biotech Co. Ltd. (Table ). The annealed oligonucleotides were ligated using T4 DNA ligase (TaKaRa) into the Tc operon of pACYC184 vector digested with Bam HI and Sph I (Huang et al, ; Qin et al, ). Recombinant plasmids, namely pACYC184‐ luxR 05 , pACYC184‐ luxR 08 , pACYC184‐ luxR 19 , pACYC184‐ luxR 11 , pACYC184‐ luxR 164 and pACYC184‐ luxR 165 , were identified by DNA sequencing and transformed into A. hydrophila by electroporation (Luo et al, ; Zhang, Luo, et al, ).…”

Section: Methodsmentioning

confidence: 99%

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

Mao

Qin

Kang

et al. 2019

Journal of Fish Diseases

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LuxR‐type transcriptional factors are essential in many bacterial physiological processes. However, there have been no reports on their roles in Aeromonas hydrophila. In this study, six stable silent strains were constructed using shRNA. Significant decreases in the expression levels of luxR05, luxR08, luxR19, luxR11, luxR164 and luxR165 were shown in their respective strains by qRT‐PCR. The luxR05‐RNAi and luxR164‐RNAi exhibit the most significant changes in sensitivity to kanamycin and gentamicin. The luxR05‐RNAi showed minimum biofilm formation and the least motility, while luxR164‐RNAi showed minimum biofilm formation, adhesion, growth and extracellular protease activity compared to the wild‐type strain. In summary, the results of this paper suggest that all six luxR genes are involved in multiple physiological processes in A. hydrophila and that the roles of luxR05 and luxR164 are highly significant. The sensitivity of luxR05‐RNAi and luxR164‐RNAi to drugs may be closely related to biofilm formation. The luxR05 may play an important role in the pathogenicity of A. hydrophila by regulating the movement, adhesion and biofilm formation of bacteria, whereas luxR164 may be involved in similar functions by regulating bacterial adhesion, extracellular enzyme activity and growth. These results help further our understanding of the drug resistance and pathogenesis of A. hydrophila.

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Section: Methodsmentioning

confidence: 99%

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

Mao

Qin

Kang

et al. 2019

Journal of Fish Diseases

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“…From the OD 600 data, growth curves were plotted comparing the wild‐type and RNAi‐treated strains. Three independent biological replicates were performed for each data point (Huang et al., ).…”

Section: Methodsmentioning

confidence: 99%

The role of rpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses

Huang

Guo

et al. 2019

Journal of Fish Diseases

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Vibrio alginolyticus is a leading aquatic pathogen, causing huge losses to aquaculture. rpoS has been proven to play a variety of important roles in stress response and virulence in several bacteria. In our previous study, upon treatment with Cu2+, Pb2+, Hg2+ and low pH, the expression levels of rpoS were downregulated as assessed by RNA‐seq, while impaired adhesion ability was observed, indicating that rpoS might play roles in the regulation of adhesion. In the present study, the RNAi technology was used to knockdown rpoS in V. alginolyticus. In comparison with wild‐type V. alginolyticus, RNAi‐treated bacteria showed significantly impaired abilities of adhesion, growth, haemolytic, biofilm production, movement and virulence. Meanwhile, alterations of temperature, salinity, pH and starvation starkly affected rpoS expression. The present data suggested that rpoS is a critical regulator of virulence in V. alginolyticus; in addition, rpoS regulates bacterial adhesion in response to temperature, pH and nutrient content changes. These are helpful to explore its pathogenic mechanism and provide reference for disease control.

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“…The water temperature during infection was 18 ± 1°C. The daily mortality of infected E. coioides was recorded [48].…”

Section: Artificial Infection and Samplingmentioning

confidence: 99%

Mechanisms underlying the virulence regulation of new Vibrio alginolyticus ncRNA Vvrr1 with a comparative proteomic analysis

Zuo

Zhao

et al. 2019

Emerging Microbes & Infections

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The incidence of Vibrio alginolyticus infections has increased in recent years due to the influence of climate change and rising sea temperature. Vibrio virulence regulatory RNA 1 (Vvrr1) is a newly found noncoding RNA (ncRNA) predicted to be closely related to the adhesion ability of V. alginolyticus based on the previous RNA-seq. In this study, the target genes of Vvrr1 were fully screened and verified by constructing Vvrr1-overexpressing strains and using the proteome sequencing technology. Pyruvate kinase I (pykF) gene was predicted to be a chief target gene of Vvrr1 involved in virulence regulation. The adhesion ability, biofilm formation and virulence were significantly reduced in the Vvrr1-overexpressing and the pykFsilenced strain compared with the wild strains. Similar to the overexpression of Vvrr1, the silencing of pykF also reduced the expression level of virulence genes, such as ndk, eno, sdhB, glpF, and cysH. Meanwhile, by constructing the "pykF-GFP" fusion expression plasmid and using the GFP reporter gene analysis in Escherichia coli, the fluorescence intensity of the strain containing Vvrr1 whole ncRNA sequence vector was found to be significantly weakened. These indicated that Vvrr1 participated in the virulence regulation mechanism of V. alginolyticus by interacting with the virulence gene pykF.

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A metabolomic investigation into the temperature‐dependent virulence of Pseudomonas plecoglossicida from large yellow croaker (Pseudosciaena crocea)

Cited by 62 publications

References 69 publications

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

The role of rpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses

Mechanisms underlying the virulence regulation of new Vibrio alginolyticus ncRNA Vvrr1 with a comparative proteomic analysis

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