Mechanisms underlying the virulence regulation of new Vibrio alginolyticus ncRNA Vvrr1 with a comparative proteomic analysis

Abstract

The incidence of Vibrio alginolyticus infections has increased in recent years due to the influence of climate change and rising sea temperature. Vibrio virulence regulatory RNA 1 (Vvrr1) is a newly found noncoding RNA (ncRNA) predicted to be closely related to the adhesion ability of V. alginolyticus based on the previous RNA-seq. In this study, the target genes of Vvrr1 were fully screened and verified by constructing Vvrr1-overexpressing strains and using the proteome sequencing technology. Pyruvate kinase I (pykF) gene was predicted to be a chief target gene of Vvrr1 involved in virulence regulation. The adhesion ability, biofilm formation and virulence were significantly reduced in the Vvrr1-overexpressing and the pykFsilenced strain compared with the wild strains. Similar to the overexpression of Vvrr1, the silencing of pykF also reduced the expression level of virulence genes, such as ndk, eno, sdhB, glpF, and cysH. Meanwhile, by constructing the "pykF-GFP" fusion expression plasmid and using the GFP reporter gene analysis in Escherichia coli, the fluorescence intensity of the strain containing Vvrr1 whole ncRNA sequence vector was found to be significantly weakened. These indicated that Vvrr1 participated in the virulence regulation mechanism of V. alginolyticus by interacting with the virulence gene pykF.

Type III Secretion 1 Effector Gene Diversity Among Vibrio Isolates From Coastal Areas in China

“…In this study, we obtained 19 different Vibrio species from the coastal areas of South China and the Jiangsu province, and the most abundant species were found to be V. parahaemolyticus, V. alginolyticus, and V. harveyi. This was in agreement with the previous reports indicating that the members of the harveyi clade are usually the dominant groups in Chinese coastal environments (Xie et al, 2005;Austin and Zhang, 2006;Chen et al, 2011;Han et al, 2015;Zuo et al, 2019).…”

Disentangling the abundance and structure of Vibrio communities in a semi-enclosed Bay with mariculture (Dongshan Bay, Southern China)

“…Primers targeting bacterial or Vibrio -specific 16S rRNA gene regions are usually used to assess the diversity of Vibrio in coastal and marine waters through high-throughput sequencing [31] , [46] , [20] , [43] , [28] , [56] , [8] , and also cultivation-dependent methods have been used [47] , [50] . In our study, we combined all three methods to evaluate consensus and differences between the obtained results.…”

Complete genome sequence of highly pathogenic Vibrio parahaemolyticus isolated from mariculture Penaeus vannamei reveals virulence factor genes

“…In aquaculture, members of the genus Vibrio are often detected during outbreaks of many serious diseases (Rui et al., 2015; Tan et al., 2019; Vezzulli et al., 2014; Zuo et al., 2019). Vibrio parahaemolyticus is a common halophilic bacterium, widely distributed in coastal waters and marine sediments and is also one of the commonest pathogens in seafood (Tsai et al., 2013).…”