“…Focusing on functionally relevant biological processes and proteins, such as tick attachment and feeding, proteases and protease inhibitors at the tick-host interface, water balance, blood digestion, heme and iron metabolism, detoxification, mating, vitellogenesis, and fertility, allowed the identification of candidate vaccine tick protective antigens [26,37]. Some of these antigens, such as Aquaporin [37][38][39][40], Ferritin [41][42][43][44], and 64TRP cement protein [45,46], have shown efficacy in vaccine formulations for the control of tick infestations, thus providing support for the potential of this predictive model for the identification of candidate protective antigens. Nevertheless, these experiments were conducted in mammalian animal models, which show differences in the immune response when compared to fish [47].…”