This study aimed to determine the discriminatory power of textural features to differentiate the sorghum grains subjected to normal, mild deficit, and severe deficit irrigation. The studies were carried out with the use of image processing, discrimination analysis, analysis of variance and cluster analysis using the selected texture parameters calculate for images from individual color channels L, a, b, R, G, B, U, V, S, X, Y and Z. The results indicated that different levels of irrigation can discriminate the sorghum grain with an accuracy of up to about 100%. Most of the genotypes for each level of irrigation were different in the terms of values of textural features and formed separate homogeneous groups. Drought is one of the limiting factors contributing to a decrease in sorghum grain productivity and nutritional quality, especially when it is cultivated in a marginal area. Therefore, low-quality grains produced under water stress should be recognized before they enter into the food and feed chain. The application of image analysis based on textures of sorghum grain images proved to be useful for the discrimination of sorghum grains subjected to drought stress. The applied procedure provided the fast, objective results that may be applied in practice for screening distinguishing the sorghum grains with different irrigation levels.