Identification and Cloning of S-Adenosylmethionine Decarboxylase (Samdc) Gene From Dictyostelium Discoideum.

Kumar, Rishikesh; Sharma, Priyanka; Saran, Shweta

doi:10.21474/ijar01/6732

Cited by 1 publication

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“…We earlier expressed the samdc gene (DDB_G0275567) as a fusion protein with the eYFP reporter at the C-terminal under actin15 promoter ( Supplementary Fig. S2C; Kumar et al, 2018). The samdc transcript levels increased till the slug stage, after which it decreased till fruiting body formation.…”

Section: Overexpression Of Samdc (Samdc Oe )mentioning

confidence: 99%

Overexpression of S-adenosylmethionine decarboxylase impacts polyamine homeostasis during development of Dictyostelium discoideum

Sharma

Kumar²,

Saran³

2018

Int. J. Dev. Biol.

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The polyamines putrescine, spermidine and spermine are essential polycations involved in the regulation of cellular proliferation. They exert dynamic effects on nucleic acids and macromolecular synthesis in vitro but their specific functions in vivo are poorly understood. Here, we have modulated the spermidine levels either by overexpressing the S-adenosylmethionine decarboxylase (samdc) gene or treating the cells with methylglyoxal-bis (guanylhydrazone) (MGBG), an inhibitor of SAMDC. In Dictyostelium, overexpression of SAMDC slowed cell proliferation, delayed development and arrested cells in the S-phase of the cell cycle. Treatment with MGBG reduced cell proliferation and stimulated development, but in samdc OE cells, it increased cell proliferation suggesting critical levels of spermidine to be important. In samdc OE cells, spermidine levels remained high throughout development but only small changes in the spermine levels were observed. Initial putrescine levels did increase but reverted to wild-type levels after the mound stage. As tight regulation of polyamine homeostasis is required, we identified genes that could be involved in its maintenance. In conclusion, we characterised samdc OE cells and observed the maintenance of polyamine homeostasis during the development of Dictyostelium cells.

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Section: Overexpression Of Samdc (Samdc Oe )mentioning

confidence: 99%