Identification and comparison of compounds in commercial Tripterygium wilfordii genus preparations with HPLC-QTOF/MS based on molecular networking and multivariate statistical analysis

Xun, Ge; Tian, Yuhuan; Gao, Yanhua; Zhang, Jia; Liu, Xin; Sun, Shuo; Qi, Qian; Liu, Fangfang; Wang, Qiao; Wang, Xu

doi:10.1016/j.jpba.2022.114811

Cited by 8 publications

(4 citation statements)

References 32 publications

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“…Among them, CH 2 CHCH 3 (42 Da) is characteristic of the loss of isopropyl groups from C-13 sites. 31…”

Section: Resultsmentioning

confidence: 99%

“…Since most structures contain carboxyl groups, they tend to lose HCOOH (46 Da) in positive ion mode and produce characteristic fragment ions. 31…”

Section: Resultsmentioning

confidence: 99%

“…Using mass spectrometry, more than 100 compounds can be simultaneously identified at present. 31 For revealing the mechanism of drug action and discovering new bioactive molecules, a new strategy for discovering and characterizing components from T. wilfordii is needed.…”

Section: Introductionmentioning

confidence: 99%

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Combining multidimensional chromatography-mass spectrometry and feature-based molecular networking methods for the systematic characterization of compounds in the supercritical fluid extract of Tripterygium wilfordii Hook F

Liu

Shen

et al. 2023

Analyst

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“…Among them, CH 2 CHCH 3 (42 Da) is characteristic of the loss of isopropyl groups from C-13 sites. 31…”

Section: Resultsmentioning

confidence: 99%

“…Since most structures contain carboxyl groups, they tend to lose HCOOH (46 Da) in positive ion mode and produce characteristic fragment ions. 31…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Combining multidimensional chromatography-mass spectrometry and feature-based molecular networking methods for the systematic characterization of compounds in the supercritical fluid extract of Tripterygium wilfordii Hook F

Liu

Shen

et al. 2023

Analyst

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“…As a main active ingredient of Tripterygium wilfordii Hook. F., and Tripterygium wilfordii multiglycoside tablet [ 7 ], a diterpene triptolide has numerous biological functions including anti-inflammation, inflammation modulation, and anti-cancer effects [ 8 ]. For instance, triptolide is a promising drug candidate for treating colon cancer [ 9 ] and reverses epithelial-mesenchymal transition in glioma cells [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

A single-cell landscape of triptolide-associated testicular toxicity in mice

Zhang

Xia

et al. 2023

Journal of Pharmaceutical Analysis

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Chemical Screening, Identification, and Comparison of Tripterygium Hypoglaucum Hutch Preparations by Ultra‐High‐Performance Liquid Chromatography‐Quadrupole Time‐of‐Flight Mass Spectrometry Combined With Multivariate Statistical Analysis

Luo,

Xiang,

Gui

et al. 2024

J of Separation Science

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Colquhounia root tablets (CRT) and Tripterygium hypoglaucum hutch tablets (THHT), two major Tripterygium hypoglaucum hutch (THH) commercial preparations, have been used to treat chronic kidney diseases or rheumatic diseases. However, there have been no reports on the chemical comparison between CRT and THHT, greatly hindering the understanding of their pharmacological difference as well as their rational application in clinical practice. In the present study, ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry combined with automated data analysis by MS‐DIAL software and MS‐FLO website was employed to systematically screen and characterize the components in CRT and THHT. Multivariate statistical analysis was used to compare the differences between these two preparations. As a result, up to 92 components were tentatively identified, and 17 of them were characterized for the first time in THH preparations. According to the criteria of variable importance in projection (VIP) >1, p < 0.05, and fold change (FC) > 1.2, 46 components could be screened as major differential chemical components. Among them, phenolic acids, organic acids, amino acids, and diterpenoids were higher in CRT, while the sesquiterpene alkaloids were relatively higher in THHT. This study clarified the chemical material basis and the difference between CRT and THHT, providing a valuable reference for quality control and clinical rational use of THH preparations.

show abstract

Identification and comparison of compounds in commercial Tripterygium wilfordii genus preparations with HPLC-QTOF/MS based on molecular networking and multivariate statistical analysis

Cited by 8 publications

References 32 publications

Combining multidimensional chromatography-mass spectrometry and feature-based molecular networking methods for the systematic characterization of compounds in the supercritical fluid extract of Tripterygium wilfordii Hook F

Combining multidimensional chromatography-mass spectrometry and feature-based molecular networking methods for the systematic characterization of compounds in the supercritical fluid extract of Tripterygium wilfordii Hook F

A single-cell landscape of triptolide-associated testicular toxicity in mice

Chemical Screening, Identification, and Comparison of Tripterygium Hypoglaucum Hutch Preparations by Ultra‐High‐Performance Liquid Chromatography‐Quadrupole Time‐of‐Flight Mass Spectrometry Combined With Multivariate Statistical Analysis

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