Identification and determination of antioxidant constituents of bioluminescent mushroom

Jose, Gilish; Radhamany, P. M.

doi:10.1016/s2221-1691(12)60194-4

Cited by 21 publications

(11 citation statements)

References 6 publications

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“…gave the lowest amount (Table 1). Total flavonoid content reported in the current study is higher than the value reported by Shirmila and Radhamany 3 . They reported a value of 0.39 mg quercitin equivalent/g of extract.…”

Section: Total Phenol and Flavonoid Contentcontrasting

confidence: 93%

“…Mushrooms are also good sources of antioxidants and are therefore possible protective agents for the reduction of oxidative damage in humans without any side effects. 3 In recent times edible mushrooms have attracted interest as functional foods as well as source of bioactive metabolites for the development of drugs and nutraceuticals. 4,5 Some have also been found to be a source of phenolic compounds, 6 flavonoids, terpenoids, sterols, ascorbic acid, ergothioneine and carotenoids.…”

Section: Introductionmentioning

confidence: 99%

“…4,5 Some have also been found to be a source of phenolic compounds, 6 flavonoids, terpenoids, sterols, ascorbic acid, ergothioneine and carotenoids. 3,4,5,6,7 Various methods are available for the screening of pharmacologically active substances in extracts. One such method is the thin layer chromatography (TLC), a simple, quick reliable and inexpensive procedure that can be used for screening of plant extracts.…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical, Free Radical Scavenging Activity and Thin Layer Chromatography Analysis of Methanolic Extracts of Six Wild Mushroom Species Collected From the Shai Hills Reserve of Ghana

Owusu¹,

Schwinger²,

Dzomeku³

et al. 2017

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Objective: Six different mushroom species (Termitomyces, Ganoderma, Amauroderma, Mycena, Marasmus and an unknown) were studied to ascertain their phytochemical and antioxidant properties and determine their TLC analysis of methanolic extracts. Materials and Methods:The DPPH (1,1-diphenyl-2-picrylhydrazyl) model was employed to determine free radical scavenging activity of the methanolic extracts of the mushrooms, aluminum chloride calorimetric method for flavonoid, Thin layer chromatography (TLC) for retention factor and atomic absorption spectrophotometric (AAS) for macro and micronutrients. Results: Phytochemical analyses of the methanolic extract revealed the presence of antioxidants, phenols and flavonoids.The antioxidant values (IC 50 (µg/ml)) ranged from 1.56 x 10 -4 to 21.07 x10 -4 .Total phenol content ranged between 2.54 and 17.53 mg/g GAE with a mean of about 11.27 mg/g GAE. Total flavonoid content also ranged from 5.46 to 23.75 mg/g RUE with a mean of 13.41 mg/g RUE. Micronutrients such as cadmium, iron, lead, manganese and zinc were determined. Also macronutrients determined included, calcium, potassium, magnesium and sodium. Sodium ranged the highest with values ranging 6966.67 mg/L to 9600.00 mg/L followed by iron ranging from 1613.67 to 3040.00 mg/L. Percentage crude protein ranged between 11.09 % and 28.24 %. Alkaloid was present in only Mycena sp and Ganoderma sp with a band each of R f value of 0.49. A total of 18 different bands were recorded for flavonoid with R f values that ranged between 0.20 and 0.97, respectively. Conclusion: The antioxidant potential of the methanolic extracts of the mushroom samples in this study recorded higher values. This indicates that mushrooms have high antioxidant properties and rationalizes further investigation in the potential discovery of new natural bioactive principles from these mushrooms.

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Section: Total Phenol and Flavonoid Contentcontrasting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phytochemical, Free Radical Scavenging Activity and Thin Layer Chromatography Analysis of Methanolic Extracts of Six Wild Mushroom Species Collected From the Shai Hills Reserve of Ghana

Owusu¹,

Schwinger²,

Dzomeku³

et al. 2017

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“…In addition to the phytochemicals, fungi have also been considered as a source of biologically active substances that can be used to reduce oxidative damage in humans and useful in disease prevention (Chye et al, 2008; Fatih et al, 2010; Adebayo et al, 2012; Shirmila and Radhamany, 2012). Fungi produce a considerable amount of metabolites, including vitamin C, vitamin E, and beta-carotene as well as phenolic compounds, known as excellent antioxidants (Barros et al, 2007; Chye et al, 2008; Yin et al, 2008; Fatih et al, 2010; Keles et al, 2011; Adebayo et al, 2012; Shirmila and Radhamany, 2012). …”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Antioxidant Activity of Aqueous and Methanol Extracts of Pleurotus ostreatus in Different Growth Stages

et al. 2016

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Total polyphenols and flavonoids contents, as well as ferric reducing antioxidant power (FRAP), metal ions chelating activity, reducing power assay and scavenging activity of DPPH and ABTS radicals in aqueous and methanolic extracts obtained from mycelium, primordium, and fruiting body of Pleurotus ostreatus in both fresh as dry, were evaluated. The total polyphenol content of dried samples was higher in aqueous extracts obtained both in room temperature and boiling. The total polyphenol content of the fresh samples obtained at room temperature and boiling was higher in aqueous extract of mycelium and in the methanolic extract of the fruiting body. In general, flavonoids represented a very small percentage of the total polyphenol content. The antioxidant activity measured by the FRAP method of extracts from fresh samples were higher with respect to the dried samples. The results of the metal ion chelating activity indicate that all extracts tested had acted. The reducing power of all samples was concentration dependent. In general, the extracts of dried samples showed higher reducing power than the extracts of fresh samples and tend to show greater reducing power by aqueous than methanolic extracts. It was observed that the DPPH and ABTS radical scavenging activities were positively correlated to the concentration of the extract. The results suggested that antioxidant activity could be due to polyphenols, but mainly by different molecules or substances present in the extracts. Overall, the fruiting body of P. ostreatus showed the best results and the possibility of continuing to investigate its functional properties of this fungus is opened. This is the first report where the antioxidant activity of P. ostreatus in different growth stage was reported.

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“…The developed plates were air dried and observed (under visible and UV light). After this examination, the antioxidant constituents were analyzed by DPPH technique [26,27]. For this 0.05 % of DPPH solution in methanol was sprayed on the surface of developed TLC plates and incubated for 10 min at room temperature.…”

Section: Thin Layer Chromatography (Tlc) Analysis For Antioxidant Conmentioning

confidence: 99%

Chemical Constituents, Antioxidative and Antibacterial Properties of Medicinal Mushrooms Collected from Similipal Biosphere Reserve, Odisha, India

Singdevsachan

Patra

Tayung

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Medicinal mushrooms viz. Ganoderma applanatum, Ganoderma lipsiense, Ganoderma chalceum and Ganoderma tsugae are important forest products of Similipal Biosphere Reserve, India. The present study aimed to evaluate the chemical constituents, antioxidant and antibacterial potential of these mushrooms. The chemical constituents were determined in terms of macronutrients, micronutrients and phenolic contents. The macronutrient content of these mushrooms revealed high amounts of protein (17.10-20.50/100 g), carbohydrate (41.80-59.16/100 g) and low fats (1.2-3.0/100 g) and possessed good quantities micronutrients (vitamins, carotenoids). Mushrooms showed strong antioxidant properties due to the presence of high phenol (27.40-220.41 mg catechol/g extract) and flavonoid (3.42-171.44 mg quercetin/g extract) content. All these mushrooms possess moderate antibacterial properties with zone of inhibition ranging from 11.76 to 18.83 mm against five human pathogenic bacteria. Based on their rich bioactive compounds (vitamins and phenolics), antioxidant and antibacterial activity, they might be exploited by pharmaceutical and cosmetic industries.

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Identification and determination of antioxidant constituents of bioluminescent mushroom

Cited by 21 publications

References 6 publications

Phytochemical, Free Radical Scavenging Activity and Thin Layer Chromatography Analysis of Methanolic Extracts of Six Wild Mushroom Species Collected From the Shai Hills Reserve of Ghana

Phytochemical, Free Radical Scavenging Activity and Thin Layer Chromatography Analysis of Methanolic Extracts of Six Wild Mushroom Species Collected From the Shai Hills Reserve of Ghana

Evaluation of the Antioxidant Activity of Aqueous and Methanol Extracts of Pleurotus ostreatus in Different Growth Stages

Chemical Constituents, Antioxidative and Antibacterial Properties of Medicinal Mushrooms Collected from Similipal Biosphere Reserve, Odisha, India

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