Identification and Determination of Nonnitrogenous Organic Acids of Sugar Cane by Partition Chromatography

Roberts, E. J.; Martin, L.F.

doi:10.1021/ac60089a007

Cited by 20 publications

(9 citation statements)

References 6 publications

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“…Tricarbailylic and trans-aconitic acids occur in nature in unripe beet root, barley, maize, and sugar beet as minor products (Nelson and Mottern, 1931;Daigliesh, Long, and Tyler, 1952;Roberts and Martin, 1954;Vavruch, 1954;Miller and Swain, 1960). trans-Aconitic acid occurs to the extent of 4 to 5% in cane molasses (Roberts and Martin, 1954). A preliminary report (Gray and Brooks, 1954) on isolation of tricarballylateoxidizing microorganisms and on their ability to oxidize the Krebs cycle intermediates and tricarballylate appeared almost 10 years ago.…”

mentioning

confidence: 99%

MICROBIOLOGICAL DISSIMILATION OF TRICARBALLYLATE AND TRANS -ACONITATE

Altekar

Rao

1963

J Bacteriol

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Altekar , W. W. (National Chemical Laboratory, Poona, India) and M. R. Raghavendra Rao . Microbiological dissimilation of tricarballylate and trans -aconitate. J. Bacteriol. 85: 604–613. 1963.—Two fluorescent pseudomonads capable of metabolizing tricarballylate and trans -aconitate were isolated by the soil-enrichment culture technique. These and some other species of bacteria were tested for their ability to utilize for growth the salts of many di- and tricarboxylic acids. Alloisocitrate and mesaconate were not utilized by any of the ten strains tested; only two strains grew on tricarballylate and itaconate. trans -Aconitate was utilized by many strains which had not been previously exposed to this compound. The resting cells of two strains could adapt to oxidize two acids (tricarballylate and trans -aconitate), and this induction was chloramphenicol-sensitive. The tricarballylate-grown cells were simultaneously induced to oxidize trans -aconitate and other tricarboxylates, whereas the trans -aconitate-grown cells were not induced to oxidize tricarballylate, and their subsequent induction was inhibited by chloramphenicol. This trans -aconitate or tricarballylate. But tricarballylate dehydrogenase was present only in tricarballylate-grown cells. The cell-free extracts of the two organisms contained the enzymes of the Krebs cycle and isocitritase. These enzymes are most probably operative during growth on and oxidation of these two acids as sole carbon sources.

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confidence: 99%

MICROBIOLOGICAL DISSIMILATION OF TRICARBALLYLATE AND TRANS -ACONITATE

Altekar

Rao

1963

J Bacteriol

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“…Material high in uranium content (greater than 5% uranium oxide) usually requires less preliminary treatment than material of low uranium content but, nevertheless, a separation is almost always necessary before a volumetric or colorimetric finish can be employed. Usually, one or more of such standard procedures as hydrogen sulfide separation, cupferron separation, ammonium or sodium carbonate separation, and mercury cathode separation are required (15). Such separations are, of course, time-consuming.…”

Section: Discussionmentioning

confidence: 99%

“…Others (3, 7) have found this to be unsatisfactory for some acids; incorporation of sulfuric acid in the gel itself effectively suppresses ionization of the organic acids, thereby greatly decreasing the amount of "tailing." On the other hand, a recent paper (15) has described a modification of the Marvel and Rands technique which still employed an unacidified column.…”

Section: Inmentioning

confidence: 99%

Chromatography of Organic Acids in Cured Tobacco

Resnik¹,

Lee²,

Powell³

1955

Anal. Chem.

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“…Similar procedures have recently been published by HOUSTON and HAMIL-TON (1952) ;BULEN, VARNER, and BURRELL (1952);ROBERTS and MARTIN (1954);and MUELLER, LARSON, and LENNARZ (1958). RESNIK, LEE, and POWELL (1955) used tertiary-butyl alcohol instead of n-butyl alcohol to make the end-point of the titration sharper and also to prevent the possibility of ester forma-tion, which is likely to occur on the column.…”

Section: Introductionmentioning

confidence: 95%