Identification and Determination of the Major Constituents in the Traditional Uighur Medicinal Plant Saussurea involucrata by LC-DAD-MS

Abstract

A high-performance liquid chromatography (LC) coupled with diode array detector (DAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for the simultaneous analysis of the major constituents in Saussurea involucrata (SI). A comprehensive validation of the developed method was conducted, and the unique properties of the present method were confirmed by analyzing eleven SI samples. Seventeen compounds including phenolic acids, flavonoids, and lignanoids were identified by online ESI-MS and by comparison with known data in the literature and standard compounds; of these, eight were simultaneously quantified by LC-DAD. All linear regressions were acquired with R 2 > 0.99, and the limits of detection ranged from 0.85 to 3.03 ng. Repeatability was evaluated by intra-and inter-day assays; the relative standard deviation (RSD) value was within 4.95%. Recovery studies for the quantified compounds were found to be within the range 95.20-102.32% with RSD less than 2.18%. Overall, the present hyphenation procedure is highly efficient and reliable, and hence suitable for qualitative and quantitative analysis of a large number of samples.

Botanicals inRibes nigrumbud-preparations: An analytical fingerprinting to evaluate the bioactive contribution to total phytocomplex

“…They describe phenolic acid determination in medicinal plants and preparations, as R. nigrum bud-preparations (Castro et al, 2010;Urpi-Sarda et al, 2009), according to the single bioactive compound concentrations shown in this research. Among other identified classes, flavonols and catechins were also selected for quantitative studies (Huang et al, 2008;Surveswaran et al, 2010;Yi et al, 2009). Based on the obtained results, most of the research pointed out that the identified polyphenolic compounds significantly contribute to the total phytocomplex of these herbal preparations.…”

Saussurea involucrata: A review of the botany, phytochemistry and ethnopharmacology of a rare traditional herbal medicine

“…The abundance of chemical constituents differs in herbs obtained from different origins. It is believed that the quality of Snow Lotus sourced from Tianshan of Xinjiang in China (Yi et al, 2009b) is the best of all.…”

“…Total phenolic content was found to be ranging from 23.58 to 41.56 mg/g while the content for total flavonoid is 9.08 and 15.84 mg/g. Other bioactive compounds were qualitatively determined as well, including syringin (0.20-0.58 mg/g), arctiin (0.05-1.62 mg/g), apigenin (0.005-0.015 mg/g) and hispidulin (0.05-0.16 mg/g) (Chen et al, 2013a;Li and Zhong, 2013;Qiu et al, 2010;Xu et al, 2009;Yi et al, 2009b).…”

“…Another possibility is to explore other species of the genus Saussurea as potential substitutes for S. involucrata. Other species are in fact currently being used, together with S. involucrata, and recent research has demonstrated common biological activities and similarities in chemotaxonomy (Yi et al, 2009a;2009b;.…”

“…Phenylpropanoids, flavonoids, coumarins, lignans, sesquiterpenes, steroids, ceramides and polysaccharides as main compositions were isolated and identified. Among them, rutin and chlorogenic acid have been proven with pharmacological evidence and active compounds in 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 abundance of S. involucrata (Yi et al, 2009b) and they are the chemical markers for its quality control in the current version of Chinese Pharmacopoeia (Chinese Pharmacopoeia Commission, 2010).…”