Identification and evaluation of antibacterial agents present in lipophilic fractions isolated from sub-products of<i>Phoenix dactilyfera</i>

Bentrad, Najla; Gaceb-Terrak, Rabéa; Rahmania, Fatma

doi:10.1080/14786419.2017.1314282

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…Date seeds are often used in alternative and folk medicine for the management of diabetes, hypertension, cancer, liver diseases, gastrointestinal, and cardiovascular disorders and also used to improve the functionality and integrity of the immune system [ 13 , 15 , 16 ]. Moderate antibacterial properties of acetone and ethanolic extract of date seed have also been reported against Bacillus cereus , Staphylococcus aureus, Enterococcus faecalis, Methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli [ 17 – 19 ]. The aim of present work was (i) the biosynthesis of AgNPs using date seed extract (DSE) as bioreductant and stabilizing agents; (ii) characterization of green synthesized AgNPs by various sophisticated techniques; (iii) evaluation of antibacterial potential of synthesized nanoparticles against Methicillin-resistant Staphylococcus aureus (MRSA ATCC 43300) using twofold serial dilution and agar well diffusion methods; and (iv) characterization of morphological and ultrastructural changes caused by AgNPs on MRSA.…”

Section: Introductionmentioning

confidence: 99%

One‐Pot Facile Green Synthesis of Silver Nanoparticles Using Seed Extract of Phoenix dactylifera and Their Bactericidal Potential against MRSA

Ansari

Alzohairy

2018

Evidence-Based Complementary and Alternative Medicine

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Due to the great economic, health, and medicinal importance, Phoenix dactylifera seeds were chosen for the synthesis of silver nanoparticles (AgNPs) because of their ecofriendly, nonhazardous, cost effectiveness advancement over physical and chemical methods, as green methods are safe, one step, and simple and did not require any chemical reducing and stabilizing agents. The green synthesized AgNPs were characterized by UV-Vis spectroscopy, SEM, HR-TEM, and DLS. Further, the bactericidal activity of synthesized AgNPs against Methicillin-resistant Staphylococcus aureus (MRSA) was investigated by determining MIC/MBC, agar diffusion methods, and electron microscopy. TEM images of the so-formed AgNPs revealed that the NPs were spherical in shape, with a size range of 14–30 nm. The MIC and MBC of AgNPs for MRSA were found to be 10.67±0.94 and 17.33±1.89 μg/ml, respectively. The antibacterial activities were found to be increased with the increasing concentration of AgNPs. The zone of inhibition was greater (24mm) at highest concentrations (500μg/ml) of AgNPs, while smaller (11mm) at lowest concentrations (7.8μg/ml). The SEM images of treated MRSA cells showed wrinkled and damaged cell wall, indicating the disruption and disorganization of membrane. HR-TEM analysis exhibits extensive injury and complete disintegration of cell wall and membrane. Large translucent zones have been seen in the cytoplasm, due to either localized or complete separation of the cell membrane from the cell wall. Overall, these results indicate that green synthesized AgNPs should be considered as an effective treatment and prevention option for the medical devises related infections caused by deadly MRSA and other drug resistant pathogens.

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Section: Introductionmentioning

confidence: 99%

One‐Pot Facile Green Synthesis of Silver Nanoparticles Using Seed Extract of Phoenix dactylifera and Their Bactericidal Potential against MRSA

Ansari

Alzohairy

2018

Evidence-Based Complementary and Alternative Medicine

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“…The antibacterial activity of the other parts of P. dactylifera were also investigated, Aldaihan and Bhat [18] published that, among different parts of date palm investigated for antibacterial properties (leaf, fruit, seed and tree bark), fruits showed the highest inhibitory activity against different Gram-positive and Gramnegative bacteria. Also, Bentrad et al [19] Studied the lipophilic fractions from seeds and pollen of P. dactylifera, some of these fractions recorded significant antibacterial activity against some bacterial pathogens.…”

Section: Discussionmentioning

confidence: 99%

Preliminary Screening for Antibacterial Properties of the Male Flowers of Phoenix dactylifera

Abdallah

2019

SAJRM

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Objective: The aim of this study was to evaluate the antibacterial potential of the male flowers of Phoenix dactylifera (date palm tree) against five Gram-positive and five Gram-negative bacteria. Methods: Male flowers were collected and extracted by maceration using 80% methanol and the antibacterial activity was determined using cup-plate diffusion test, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests. Results: The methanol extract of male flowers of Phoenix dactylifera showed varying degrees of antibacterial activity against tested bacterial strains, the most susceptible Gram-positive bacteria were Bacillus cereus and Streptococcus pneumonia which recorded 12.2±0.3 and 9.0±0.0 mm zone of inhibition (ZI), MIC values were 50 and 100 mg/ml, MBC values were 200 and ˂200 mg/ml, respectively. The most susceptible Gram-negative bacteria were Proteus vulgaris, Klebsiella pneumonia and Pseudomonas aeruginosa which recorded 10.0±0.0, 9.7±0.3 and 9.0±0.0 mm ZI, MIC values were 100 mg/ml and MBC values were 200 mg/ml, respectively. Based on MBC/MIC ratio, the extract has some degree of bactericidal effect. However, the results were not competitive with the standard drug (Chloramphenicol). Conclusion: As a result, the tested methanol extract of male flowers of date palm tree exhibited some degree of antibacterial activity with a bactericidal property. More future studies such as fractionation process are required to isolate and investigate its bioactive compounds.

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“…The MIC of CHCl 3 extract that inhabited 10 mm of Serratia marcescens (-ve) and pseudomonas aeruginosa (-ve) were 50 and 25 μg/mL, respectively. The presence of polyunsaturated fatty acids especially linoleic acid and terpenes in CHCl 3 extract might be the reason for the antibacterial activity (Bentrad et al, 2017;Gallucci et al, 2009). The inhibition zones of 100 µg/mL of ethyl acetate extract on geotrichum candidum, trichophyton rubrum, fasarium oxysporum, and aspergillus flavus were 14, 22, 14, and 13, respectively.…”

Section: Antimicrobial Activitiesmentioning

confidence: 99%

Phytochemical Screening And Biological Activities Of Tamr Dry Date Variety Cultivated In Upper Egypt

Soliman

El-Sayed

et al. 2021

International Journal of Fruit Science

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Egypt is considered one of the main producers of date fruits in the world. Tamr dry date is widely cultivated in Upper Egypt, however, investigating the chemical components and biological activities of such common variety has not been performed yet. In this study, the chemical components of the Tamr variety and its biological activities were investigated. Results showed that the percentages of sugar, dietary fibers, metal, protein were 61.0%, 13.7%, 2.1, and 2.6%, respectively. Total phenolic and flavonoids were 17.6 and 2.3 mg/ 100 g, respectively. 17 amino acids, 8 metals, 15 fatty acids, 30 terpenes, vitamins (A, B1, B2, B3), and 5 flavonoids were identified. The presence of phenolics and flavonoids in ethyl acetate extract resulted in high biological activity than chloroform and methanolic extracts. The biological activity of chloroform extract could be ascribed to the presence of polyunsaturated fatty acids and terpenes. These results are in good agreement with the chemical-biological relationships.

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Identification and evaluation of antibacterial agents present in lipophilic fractions isolated from sub-products ofPhoenix dactilyfera

Cited by 11 publications

References 23 publications

One‐Pot Facile Green Synthesis of Silver Nanoparticles Using Seed Extract of Phoenix dactylifera and Their Bactericidal Potential against MRSA

One‐Pot Facile Green Synthesis of Silver Nanoparticles Using Seed Extract of Phoenix dactylifera and Their Bactericidal Potential against MRSA

Preliminary Screening for Antibacterial Properties of the Male Flowers of Phoenix dactylifera

Phytochemical Screening And Biological Activities Of Tamr Dry Date Variety Cultivated In Upper Egypt

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