Identification and Expression Analysis of the bHLH Gene Family Members in Diospyros kaki

Han, Weijuan; Zhang, Qi; Suo, Yujing; Li, Huawei; Sun, Peng; Huang, Lin; Fu, Jianmin

doi:10.3390/horticulturae9030380

Cited by 5 publications

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sites of protein structural domains were obtained according to the NCBI online software (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi, accessed on 11 March 2023) [44], and then the DOG software (Version 2.0) was used to draw the schematic diagram of protein structural domains [45].…”

Section: Protein Structural Domain and Motif Analysismentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of the High-Mobility Group B (HMGB) Gene Family in Plant Response to Abiotic Stress in Tomato

Zheng,

Tang,

Wang

et al. 2024

IJMS

View full text Add to dashboard Cite

High-mobility group B (HMGB) proteins are a class of non-histone proteins associated with eukaryotic chromatin and are known to regulate a variety of biological processes in plants. However, the functions of HMGB genes in tomato (Solanum lycopersicum) remain largely unexplored. Here, we identified 11 members of the HMGB family in tomato using BLAST. We employed genome-wide identification, gene structure analysis, domain conservation analysis, cis-acting element analysis, collinearity analysis, and qRT-PCR-based expression analysis to study these 11 genes. These genes were categorized into four groups based on their unique protein domain structures. Despite their structural diversity, all members contain the HMG-box domain, a characteristic feature of the HMG superfamily. Syntenic analysis suggested that tomato SlHMGBs have close evolutionary relationships with their homologs in other dicots. The promoter regions of SlHMGBs are enriched with numerous cis-elements related to plant growth and development, phytohormone responsiveness, and stress responsiveness. Furthermore, SlHMGB members exhibited distinct tissue-specific expression profiles, suggesting their potential roles in regulating various aspects of plant growth and development. Most SlHMGB genes respond to a variety of abiotic stresses, including salt, drought, heat, and cold. For instance, SlHMGB2 and SlHMGB4 showed positive responses to salt, drought, and cold stresses. SlHMGB1, SlHMGB3, and SlHMGB8 were involved in responses to two types of stress: SlHMGB1 responded to drought and heat, while SlHMGB3 and SlHMGB8 responded to salt and heat. SlHMGB6 and SlHMGB11 were solely regulated by drought and heat stress, respectively. Under various treatment conditions, the number of up-regulated genes significantly outnumbered the down-regulated genes, implying that the SlHMGB family may play a crucial role in mitigating abiotic stress in tomato. These findings lay a foundation for further dissecting the precise roles of SlHMGB genes.

show abstract

Section: Protein Structural Domain and Motif Analysismentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of the High-Mobility Group B (HMGB) Gene Family in Plant Response to Abiotic Stress in Tomato

Zheng,

Tang,

Wang

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…Identification of bHLH family genes was initially recorded in maize (Zea mays L.) [11]. Thereafter, an increasing number of bHLH genes were widely characterized in numerous plant species, such as Arabidopsis [12], rice [13], tomato [14], poplar [1], apple [15], eggplant [16], grass pea [17], persimmon [18], sweet wormwood [19] and Ipomoea aquatica [20].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Expression of CmobHLH Genes in Pumpkin

Luo

Wang

et al. 2023

Horticulturae

View full text Add to dashboard Cite

The transcription factor bHLH gene family plays fundamental roles in plant development and mitigating diverse biotic and abiotic stresses. However, the information of bHLH genes in pumpkin (Cucurbita moschata) is still unknown. In this current study, 222 CmobHLH genes were identified and mapped onto different chromosomes through bioinformatics analysis in pumpkin. CmobHLH and AtbHLH proteins could be classified into 19 subfamilies according to the phylogenetic tree. CmobHLH proteins within the same subfamily had similar motif composition and gene structures. Gene ontology (GO), cis-regulatory elements (CREs) and protein–protein interaction analyses suggested the potential regulatory roles of CmobHLH genes during the plant development process and abiotic stresses response in pumpkin. Tissue expression patterns based on transcriptome data demonstrated that CmobHLH genes were involved in pumpkin development process, and they had unique functions in different tissues. The expression patterns of five selected CmobHLH genes after exposure to abiotic stresses showed that the CmobHLH genes played varied roles in the stress responses of pumpkin to NaCl, waterlogging, cold, ABA and drought. In brief, these findings offer important information for further functional research of CmobHLH genes and resistance breeding in pumpkin.

show abstract

Genome-Wide Identification and Characterization of bHLH Transcription Factors in Zanthoxylum bungeanum and Their Expression Analysis in Response to Salt Stress

Zhang,

Nie,

Liang

et al. 2024

Plant Mol Biol Rep

View full text Add to dashboard Cite

Identification and Expression Analysis of the bHLH Gene Family Members in Diospyros kaki

Cited by 5 publications

References 39 publications

Genome-Wide Identification and Expression Analysis of the High-Mobility Group B (HMGB) Gene Family in Plant Response to Abiotic Stress in Tomato

Genome-Wide Identification and Expression Analysis of the High-Mobility Group B (HMGB) Gene Family in Plant Response to Abiotic Stress in Tomato

Molecular Characterization and Expression of CmobHLH Genes in Pumpkin

Genome-Wide Identification and Characterization of bHLH Transcription Factors in Zanthoxylum bungeanum and Their Expression Analysis in Response to Salt Stress

Contact Info

Product

Resources

About