Identification and expression analysis of group III WRKY transcription factors in cotton

Liu, Dou; Guo, Yanliang; Evans, Ondati; Pang, Chaoyou; Wei, Hengling; Song, Meizhen; Fan, Shiliang; Yu, Shuxun

doi:10.1016/s2095-3119(15)61306-5

Cited by 13 publications

(12 citation statements)

References 52 publications

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“…Biotic stresses include insect pests, fungi, bacteria and viruses, and abiotic stresses include heat, cold, drought, salinity and wounding 1–4 . Being sessile organisms, plants sense these stresses through complex signal transduction networks and have evolved specific defensive strategies to adapt to environmental fluctuations during their long evolutionary process 5,6 . Transcriptional regulation is a major mechanism for organisms to regulate their gene expression in response to physiological or environmental stimuli 6 .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide characterization of the WRKY gene family in cultivated strawberry (Fragaria × ananassa Duch.) and the importance of several group III members in continuous cropping

Chen

Liu

2019

Sci Rep

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WRKY transcription factors play important roles in many plant developmental processes and adaptation to the environment. However, little knowledge is available about the WRKY gene family in cultivated strawberry ( Fragaria × ananassa Duch.), an important soft fruit worldwide. In this study, a total of 47 WRKY gene members were identified and renamed on the basis of their order on the chromosomes. According to their evolutionary events and conserved structure, the 47 FaWRKYs were divided into three major groups with several subgroups. A cis -element analysis showed that all FaWRKYs possessed at least one stress response-related cis -element. Comprehensive analysis, including phylogenetic analysis and expression profiling, based on real-time qPCR analysis in root, stem, leaf and fruit was performed on group III FaWRKY genes. The phylogenetic tree of the WRKY III genes in cultivated strawberry, wild Strawberry, Arabidopsis, tomato, and rice was divided into five clades. Additionally, the expression profiles of the FaWRKY genes in response to continuous cropping were further investigated based on RNA-seq data. FaWRKY25 , FaWRKY32 , and FaWRKY45 , which are group III Fa WRKY genes, were upregulated after continuous cropping. The level of reactive oxygen species (ROS) and the expression levels of PR1 and peroxidase were higher in continuous cropping (CC) than in non-continuous cropping (NCC). The results indicated that group III FaWRKYs might play an important role in continuous cropping. These results provide a foundation for genetic improvements for continuous cropping tolerance in cultivated strawberry.

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Section: Introductionmentioning

confidence: 99%

Genome-wide characterization of the WRKY gene family in cultivated strawberry (Fragaria × ananassa Duch.) and the importance of several group III members in continuous cropping

Chen

Liu

2019

Sci Rep

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“…In Arabidopsis , several ageing-related WRKY genes have been studied, among which AtWRKY30 , AtWRKY53 , AtWRKY54 and AtWRKY70 belong to the group III subfamily [24]. Given the important roles of the group III subfamily in leaf senescence, the group III subfamily was further analysed in cotton, and GhWRKY27 was identified as a member of this subfamily [33]. In the present study, multiple sequence alignment and evolutionary analysis further demonstrated that GhWRKY27 belongs to the group III subfamily.…”

Section: Discussionmentioning

confidence: 99%

“…Four marker genes of leaf senescence, AtNAP , AtORE1 , AtSAG12 and AtSAG13 , are significantly upregulated in transgenic Arabidopsis , and overexpression of GhWRKY27 accelerates leaf senescence in transgenic Arabidopsis plants, which further demonstrates the positive role of GhWRKY27 during leaf senescence. In our previous work, the expression level of GhWRKY27 was significantly elevated after SA treatment [33], suggesting the important role of this gene in SA-related stress response.…”

Section: Discussionmentioning

confidence: 99%

The WRKY transcription factor GhWRKY27 coordinates the senescence regulatory pathway in upland cotton (Gossypium hirsutum L.)

Liu

Guo

et al. 2019

BMC Plant Biol

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Background Premature senescence can reduce the yield and quality of crops. WRKY transcription factors (TFs) play important roles during leaf senescence, but little is known about their ageing mechanisms in cotton. Results In this study, a group III WRKY TF, GhWRKY27 , was isolated and characterized. The expression of GhWRKY27 was induced by leaf senescence and was higher in an early-ageing cotton variety than in a non-early-ageing cotton variety. Overexpression of GhWRKY27 in Arabidopsis promoted leaf senescence, as determined by reduced chlorophyll content and elevated expression of senescence-associated genes (SAGs). Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that GhWRKY27 interacted with an MYB TF, GhTT2 . Putative target genes of GhWRKY27 were identified via chromatin immunoprecipitation followed by sequencing (ChIP-seq). Yeast one-hybrid (Y1H) assay and electrophoretic mobility shift assay (EMSA) revealed that GhWRKY27 binds directly to the promoters of cytochrome P450 94C1 ( GhCYP94C1 ) and ripening-related protein 2 ( GhRipen2–2 ). In addition, the expression patterns of GhTT2 , GhCYP94C1 and GhRipen2–2 were identified during leaf senescence. Transient dual-luciferase reporter assay indicated that GhWRKY27 could activate the expression of GhCYP94C1 and GhRipen2–2 . Conclusions Our work lays the foundation for further study of the functional roles of WRKY genes during leaf senescence in cotton. In addition, our data provide new insights into the senescence-associated mechanisms of WRKY genes in cotton. Electronic supplementary material The online version of this article (10.1186/s12870-019-1688-z) contains supplementary material, which is available to authorized users.

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“…Furthermore, 6 WRKY domains belonged to group III, which is widely considered to be the most advanced in terms of evolution and the most relevant to adaptability (Dou et al, 2016;Kalde et al, 2003;Huang et al, 2016).…”

Section: Phylogenetic Relationship and Classification Of Mowrky Genesmentioning

confidence: 99%

“…Group III WRKY genes have only been characterized in flowering plants, and a large number of duplications and diversifications in this group appear to have resulted from different selection challenges (Dou et al, 2016;Kalde et al, 2003). To explore the evolutionary relationships of group III WRKY genes across drumstick and other land plant species, we performed a multiple sequence alignment among the 81 group III WRKY proteins from drumstick and another 7 species.…”

Section: Rapid Expansion Of Group III Wrky Genes In Land Plantsmentioning

confidence: 99%

Peer Review #1 of "Genome-wide analysis of the WRKY gene family in drumstick (Moringa oleifera Lam.) (v0.1)"

2019

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Identification and expression analysis of group III WRKY transcription factors in cotton

Cited by 13 publications

References 52 publications

Genome-wide characterization of the WRKY gene family in cultivated strawberry (Fragaria × ananassa Duch.) and the importance of several group III members in continuous cropping

Genome-wide characterization of the WRKY gene family in cultivated strawberry (Fragaria × ananassa Duch.) and the importance of several group III members in continuous cropping

The WRKY transcription factor GhWRKY27 coordinates the senescence regulatory pathway in upland cotton (Gossypium hirsutum L.)

Peer Review #1 of "Genome-wide analysis of the WRKY gene family in drumstick (Moringa oleifera Lam.) (v0.1)"

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