Identification and expression analysis of DXS1 gene isolated from Aconitum balfourii Stapf.

Sharma, Eti; Pandey, Saurabh; Gaur, Anupama

doi:10.1007/s11738-016-2239-y

Cited by 7 publications

(3 citation statements)

References 30 publications

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“…The DXS genes have been reported frequently not only from the model plant, Arabidopsis thaliana ( Carretero-Paulet et al, 2006 ), but also from ornamental plants like Lavandula latifolia ( Munoz-Bertomeu et al, 2006 ), Pelargonium spp. ( Jadaun et al, 2017 ), Catharanthus roseus ( Chahed et al, 2000 ), and Aquilaria sinensis ( Xu et al, 2014 ) and from crop plants, such as Solanum lycopersicum ( Lois et al, 2000 ), Zea mays ( Cordoba et al, 2011 ), Aconitum balfourii ( Sharma et al, 2016 ), Tripterygium wilfordii ( Tong et al, 2015 ), and Salvia miltiorrhiza ( Zhou et al, 2016 ). Because of their relationship in catalyzing a subsequent step in the MEP pathway, the DXRs are always illustrated together with the DXS genes in A. thaliana ( Carretero-Paulet et al, 2006 ), Dendrobium officinale ( Fan et al, 2016 ), Rosa rugosa ( Feng et al, 2014 ), L .…”

Section: Introductionmentioning

confidence: 99%

Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers

et al. 2018

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Lilium, the famous and significant cut flower, emits a variety of volatile organic compounds, which mainly contain monoterpenes, such as myrcene, (E)-β-ocimene, and linalool. To understand the molecular mechanism of monoterpene synthesis in Lilium, we cloned two potential genes in the methylerythritol 4-phosphate pathway, namely LiDXS and LiDXR, from the strong-flavored oriental Lilium ‘Siberia’ using a homology-based PCR strategy. The expression levels of LiDXS and LiDXR were consistent with the emission and accumulation of monoterpenes in different floral organs and during the floral development, indicating that these two genes may play key roles in monoterpene synthesis. Subcellular localization demonstrated that LiDXS and LiDXR are expressed in the chloroplasts. Ectopic expression in transgenic tobacco suggested that the flowers of LiDXS and LiDXR transgenic lines accumulated substantially more diterpene, sclareol, compared to the plants transformed with empty vector. Surprisingly, increased content of the monoterpene, linalool and sesquiterpene, caryophyllene, were detected in the LiDXR transgenic lines, whereas the emission of caryophyllene, increased in one of the LiDXS transgenic tobacco lines, indicating that these two genes play significant roles in the synthesis of floral volatiles in the transgenic plants. These results demonstrate that LiDXR can contribute to monoterpene biosynthesis in Lilium ‘Siberia’; however, the role of LiDXS in the biosynthesis of monoterpenes needs further study.

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Section: Introductionmentioning

confidence: 99%

Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers

et al. 2018

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“…Additionally, the high DXPS expression level mainly increases the contents of other terpenoids, such as diterpene, rather than saikosaponin and triterpenoids. Moreover, DXPS showed a higher expression level in leaves than in roots of several medicinal plants ( Sharma et al, 2016 ). This indicated that DXPS contributed more to the synthesis of other terpenoids compared with saikosaponin and triterpenoids.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)

et al. 2021

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The root of Panax notoginseng (Burk.), in which saponins are the major active components, is a famous traditional Chinese medicine used to stop bleeding and to decrease inflammation and heart disease. Inflorescence removal increases the yield and quality of P. notoginseng, but the underlying molecular mechanisms are unknown. Here, the differences between inflorescence-removal treatment and control groups of P. notoginseng were compared using transcriptomics and metabolomics analyses. Illumina sequencing of cDNA libraries prepared from the rhizomes, leaves and roots of the two groups independently identified 6,464, 4,584, and 7,220 differentially expressed genes (DEG), respectively. In total, 345 differentially expressed transcription factors (TFs), including MYB and WRKY family members, were induced by the inflorescence-removal treatment. Additionally, 215 DEGs involved in saponin terpenoid backbone biosynthetic pathways were identified. Most genes involved in the mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathways were activated by inflorescence removal. The co-expression analysis showed that the low expression levels of flavonoid biosynthesis-related genes (e.g., C4H and F3H) decreased the biosynthesis and accumulation of some flavonoids after inflorescence removal. The results not only provide new insights into the fundamental mechanisms underlying the poorly studied inflorescence-removal process in P. notoginseng and other rhizome crops, but they also represent an important resource for future research on gene functions during inflorescence-removal treatments and the reproductive stage.

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“…HMGS had been reported in different medicinal plants viz. Arabidopsis thaliana [12], Chamaemelum nobile [13], Matricaria chamomilla [14], Tripterygium wilfordii [8], Santalum album [15], Ginkgo biloba [16,17], Panax notoginseng [18], Taxus media [19], Stereum hirsutum [5], Ganoderma lucidum [20], Solanum tuberosum [21], Gossypium hirsutum [22], Aconitum balfourii [23]. HMGS have not been reported in C. asiatica till date.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Characterization of Triterpenoid Biosynthetic Pathway Gene HMGS in Centella Asiatica (Linn.)

Rahman

Afroz

Warsi

et al. 2022

Preprint

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The flux of isoprenoids and the total accumulation of triterpenoid saponins known as centellosides in C. asiatica are controlled by the key genes of the Mevalonate pathway (MVA). These genes were reported to have positive regulation of the pathway in providing isoprenoid moieties. For the study, full-length pathway gene Hydroxymethyl glutaryl-CoA-synthase (CaHMGS; GenBank accession number: MZ997833), was isolated from previously annotated transcriptome data of Centella asiatica leaves. HMGS has been successfully cloned and heterologously expressed in bacteria E. coli strain DH5α. The cloned gene has been sequenced and further characterized through in silico studies by different bioinformatics tools. Also, the gene sequences have been submitted in NCBI. In silico studies of isolated gene sequence revealed the nature, characteristics of genes. The ORF of HMGS is 1449 bp encoding 482 amino acids. Predicted molecular weight (MW) of HMGS was 48.09 kDa and theoretical pI was 5.97. Blast results and Multiple sequence alignments of the gene showing the similarity with HMGS of other plants of their respective families. The Molecular Evolutionary Genetic Analysis (MEGA) version 10.1.6 was used to construct a phylogenetic tree. Differential tissue-specific expression of different plant parts was also checked. Tissue expression patterns unveiled that the highest expression level of the CaHMGS had been seen in the roots and lowest in the node of the plant. Functional complementation experiment of the CaHMGS in Saccharomyces cerevisiae wild strain YSC1021 and haploid strain YSC1021 which lack HMGS protein confirmed that the CaHMGS gene encodes functional CaHMGS that catalyzed the biosynthesis of mevalonate in yeast. Though, some information is available on the pathway and secondary metabolites. However, most of the pathway steps are not characterized functionally. As the genes were not reported in Centella asiatica yet. Hence, understanding this biosynthetic pathway gene will further help in the improvement of plants for enhanced secondary metabolites production.

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Identification and expression analysis of DXS1 gene isolated from Aconitum balfourii Stapf.

Cited by 7 publications

References 30 publications

Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers

Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers

Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)

Molecular Cloning and Characterization of Triterpenoid Biosynthetic Pathway Gene HMGS in Centella Asiatica (Linn.)

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