Identification and expression of Toll-like receptors 1–10 in selected bovine and ovine tissues

Menzies, Moira; Ingham, Aaron

doi:10.1016/j.vetimm.2005.06.014

Cited by 152 publications

(94 citation statements)

References 37 publications

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“…Previous studies have shown that bovine TLRs are differentially expressed in variety of tissues (4). Above all, this study examined a clear dissociation of either cytokines or TLRs mRNA expressions by TLR agonist (LPS) stimulation in bovine monocytes and monocyte-derived macrophages.…”

Section: Discussionmentioning

confidence: 97%

“…The first strand cDNAs were synthesized using the Superscript III reverse transcriptase (RT; Invitrogen, Carlsbad, CA) with the oligo (dT) primer. For PCR, oligonucleotide primers were used as previously described (4,11,12) and are listed in Table 1. PCR was performed using the TLRs and cytokine primers.…”

Section: Rna Extraction and Semi-quantitative Rt-pcrmentioning

confidence: 99%

“…Recently, the partial sequences for bovine TLR1-10 were published, and these sequences also seem to share a high homology to ovine TLRs (4). A report indicates that TLR family members are expressed differentially among immune cells and appear to respond to different stimuli, but their protein and mRNA expression patterns are still largely unknown (5).…”

Section: Introductionmentioning

confidence: 99%

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Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves

2009

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In this experiment Toll-like receptor expression pattern in monocytes and monocyte-derived macrophages by lipopolysaccharide (LPS) stimulation was examined. Jugular venous blood was collected from four Japanese calves, and the peripheral blood mononuclear cells (PBMCs) were isolated. The cells were directly used for collecting monocytes by magnetic cell sorting or cultured for 7 days to collect monocyte-derived macrophages in Repcell. Then we analyzed the mRNA expression pattern of TLRs and cytokines in monocytes and monocyte-derived macrophages after LPS stimulation for 24 h. LPS stimulation of both monocytes and monocyte-derived macrophages resulted in an increase in the levels of mRNA transcripts for TNF-, IL-6 and IL-8. Moreover, TNF- and IL-6 mRNA expressions were significantly augmented by LPS stimulation in monocyte-derived macrophages. TLRs mRNA expressions were unchanged after LPS stimulation of monocytes, while TLRs mRNA expressions in monocyte-derived macrophages were complicated. TLR1, 3, 5, 8 and 10 were significantly decreased after LPS stimulation and there were no differences in the mRNA expressions of TLR2, 4, 6 and 7 between the groups of control and LPS stimulation. Besides, no expression of TLR9 was found. As antigen presenting cells, monocytes and monocyte-derived macrophages respond differently to LPS, so they may have different functions in the innate immune system. Cellular & Molecular Immunology. 2009;6(3):223-229.

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Section: Discussionmentioning

confidence: 97%

Section: Rna Extraction and Semi-quantitative Rt-pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves

2009

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“…The functional transcripts for TLR1-TLR9 previously identified in ruminants (Menzies & Ingham 2006, Chang et al 2009) are expressed by cells of the ovine endometrium in a temporal and cell-specific manner during the estrous cycle and early pregnancy. The abundance of TLRs was maximum in the endometrium between Days 12 and 14 of the estrous cycle and pregnancy, but then decreased on Day 16 in cyclic ewes while remaining similar in abundance in pregnant ewes.…”

Section: Discussionmentioning

confidence: 99%

“…Of the 13 TLRs identified in mammals (Hansen et al 2011, Jungi et al 2011, ten are known to be expressed in domestic ruminants (Menzies & Ingham 2006, Chang et al 2009), which display high homology with their human counterparts (Nalubamba et al 2007, Tirumurugaan et al 2010. Besides TLR10, for which no function has been established, TLR ligand specificity depends on cellular location with some being expressed on the cell surface (TLR1, TLR2, TLR4, TLR5, and TLR6) for recognition of bacterial and fungal compounds, and others being localized to the endosomal compartment (TLR3, TLR7, TLR8, and TLR9), where they mainly bind nucleic acids from viral pathogens , Kumar et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Involvement of TLR7 and TLR8 in conceptus development and establishment of pregnancy in sheep

Ruiz-Gonzalez¹,

Minten²,

Wang³

et al. 2015

Reproduction

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Toll-like receptors (TLRs) belong to the innate immune system and regulate inflammatory events that affect mammalian reproduction.In Study 1, we demonstrated that abundance of ovine TLR1-TLR9 mRNAs in the uterus differs due to reproductive status (TLR2, TLR3, TLR7, and TLR8) and the day of the estrous cycle and pregnancy (TLR1-TLR3, TLR5-TLR7, and TLR9). Expression of TLR7 and TLR8 proteins was localized primarily to uterine epithelia and stroma and regulated in a temporal manner. In Study 2, we determined that ovine conceptuses express TLR7 and TLR8 on all days studied and that expression of the envelope protein of ovine endogenous retrovirus (enJSRV-Env) declined in conceptus trophectoderm from Day 13 to Day 16 of pregnancy. In Study 3, loss-of-function experiments were conducted in vivo using morpholino antisense oligonucleotides (MAOs) injected into the uterine lumen to block synthesis of TLR7 and TLR8 proteins, individually and jointly. Conceptuses were recovered on Day 16 to assess their morphology. MAO-treated conceptuses were developmentally retarded, produced less interferon tau (IFNT), and had fewer binucleate cells (BNCs) compared with MAO-Controls. Moreover, expression of enJSRV-Env mRNA in MAO-TLR7 conceptuses was greater than that for MAO-Control and MAO-TLR8 conceptuses, but similar to MAO-TLR7/TLR8 conceptuses. Results of this study indicated differences in TLR1-TLR9 expression due to reproductive status and the day of the estrous cycle and pregnancy. TLR7 and TLR8 also influence development, enJSRV-Env abundance, secretion of IFNT, and formation of BNCs by conceptuses. These findings corroborate our hypothesis that TLR7 and TLR8 mediate pathways whereby enJSRV-Env regulates key peri-implantation events in conceptus development and differentiated functions of trophectoderm cells.

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Cell-Specific Expression Pattern of Toll-Like Receptors and Their Roles in Animal Reproduction

Atlı

Hitit

Özbek

et al. 2022

Handbook of Experimental Pharmacology

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Identification and expression of Toll-like receptors 1–10 in selected bovine and ovine tissues

Cited by 152 publications

References 37 publications

Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves

Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves

Involvement of TLR7 and TLR8 in conceptus development and establishment of pregnancy in sheep

Cell-Specific Expression Pattern of Toll-Like Receptors and Their Roles in Animal Reproduction

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