Identification and Expression Profile Analysis of Odorant Binding Proteins in the Oriental Fruit Fly Bactrocera dorsalis

Zheng, Weiwei; Peng, Wei; Zhu, Cuizhen; Zhang, Qun; Saccone, Giuseppe; Zhang, Hongyu

doi:10.3390/ijms140714936

Cited by 59 publications

(77 citation statements)

References 40 publications

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“…Specifically, we considered: ten OBPs from B. dorsalis [47] and BdorOBP2 (unpublished, GenBank accession no. AGO28153), nine OBPs from R. suavis [48], and fifteen OBPs from R. pomonella [49].…”

Section: Methodsmentioning

confidence: 99%

Sniffing Out Chemosensory Genes from the Mediterranean Fruit Fly, Ceratitis capitata

et al. 2014

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The Mediterranean fruit fly, Ceratitis capitata (medfly), is an extremely invasive agricultural pest due to its extremely wide host range and its ability to adapt to a broad range of climatic conditions and habitats. Chemosensory behaviour plays an important role in many crucial stages in the life of this insect, such as the detection of pheromone cues during mate pursuit and odorants during host plant localisation. Thus, the analysis of the chemosensory gene repertoire is an important step for the interpretation of the biology of this species and consequently its invasive potential. Moreover, these genes may represent ideal targets for the development of novel, effective control methods and pest population monitoring systems. Expressed sequence tag libraries from C. capitata adult heads, embryos, male accessory glands and testes were screened for sequences encoding putative odorant binding proteins (OBPs). A total of seventeen putative OBP transcripts were identified, corresponding to 13 Classic, three Minus-C and one Plus-C subfamily OBPs. The tissue distributions of the OBP transcripts were assessed by RT-PCR and a subset of five genes with predicted proteins sharing high sequence similarities and close phylogenetic affinities to Drosophila melanogaster pheromone binding protein related proteins (PBPRPs) were characterised in greater detail. Real Time quantitative PCR was used to assess the effects of maturation, mating and time of day on the transcript abundances of the putative PBPRP genes in the principal olfactory organs, the antennae, in males and females. The results of the present study have facilitated the annotation of OBP genes in the recently released medfly genome sequence and represent a significant contribution to the characterisation of the medfly chemosensory repertoire. The identification of these medfly OBPs/PBPRPs permitted evolutionary and functional comparisons with homologous sequences from other tephritids of the genera Bactrocera and Rhagoletis.

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Section: Methodsmentioning

confidence: 99%

Sniffing Out Chemosensory Genes from the Mediterranean Fruit Fly, Ceratitis capitata

et al. 2014

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“…Additionally, several OBPs are female-biased expression, such as OBP1, OBP2, OBP3, OBP4, OBP5, OBP8, OBP9, OBP10, and OBP13. These may be involved in female-specific chemosensory processes, such as laying eggs [20]. Among these OBPs, OBP5 is barely detected in male antennae and may be involved in recognition of male-specific pheromones.…”

Section: Discussionmentioning

confidence: 99%

Novel odorant-binding proteins and their expression patterns in grasshopper, Oedaleus asiaticus

Zhang

Pang

Zhang

2015

Biochemical and Biophysical Research Communications

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“…A phylogenetic tree was generated by obtaining the amino acid sequences of the ten putative OBPs of B. dorsalis from the work of (Zheng et al, 2013). The phylogenetic tree was built to demonstrate the relationships of the 10 BdorOBPs to 140 OBP sequences from 7 different orders and families of insects that possess the same OBP family, including 20 OBP sequences from Apis mellifera (Hymenoptera: Apidae), 7 OBP sequences from Bombyx mori (Lepidoptera: Bombycidae), 30 OBP sequences Idrees et (Figure 2).…”

Section: Phylogenetic Analysis Of Insect Obpsmentioning

confidence: 99%

“…The results of that study revealed that volatiles could potentially evoke higher relative EAG values in the antennae of B. dorsalis during three adult physiological stages (Jang and Light, 1991;Du et al, 2015). A total of 20 BdorOBP genes have been identified from the B. dorsalis transcriptome; however, only 10 BdorOBPs have been characterized (Zheng et al, 2012b(Zheng et al, , 2013.…”

Section: Introductionmentioning

confidence: 99%

“…A variety of methods such as the sterile insect technique, mass trapping, protein bait spraying and male annihilation have been developed to suppress or eradicate B. dorsalis and related species throughout Asia and the Pacific regions (Jessup et Chemoreception is an important characteristic in the selection of host plants by phytophagous insects. Odorant-binding proteins (OBPs) present in the olfactory system play an essential role in sensitivity to odorants, such as pheromones and host chemicals (Zheng et al, 2013). The olfactory system in insects consists of three central types of proteins: OBPs, odorant receptors (ORs) and odorant-degrading esterases (ODEs) (Justice et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Protein Baits, Volatile Compounds and Irradiation Influence the Expression Profiles of Odorantbinding Protein Genes in Bactrocera Dorsalis (Diptera: Tephritidae)

Idrees¹

2017

Appl. Ecol. Env. Res.

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Idrees et al.: Protein baits, volatile compounds and irradiation influence the expression profiles of odorant-binding protein genes inBactrocera dorsalis Fuzhou, Fujian, 350002, *Corresponding author e-mail: jiqinge@yeah.net; phone: +86-0-59-183-789-420; fax:+86-0-59-183-789-421 (Received 4 th Jul 2017; accepted 18 th Oct 2017)Abstract. Chemical communication in insects is based on odorant-binding proteins (OBPs), but the molecular mechanisms by which these OBPs allow the perception of volatiles and host chemicals remain unclear. Bactrocera dorsalis (Hendel) is one of the most economically important pests of fruits and vegetables. In this study, the OBPs of B. dorsalis (BdorOBPs) were evaluated in response to different attractive protein baits, brewer's yeast volatile compounds and irradiation. The expression of 10 genes encoding OBPs in the antennae of B. dorsalis during three adult physiological life stages (pre-mating, post-mating and post-oviposition) were analysed. All selected BdorOBP genes were found to contain one conserved pheromone-binding protein/general-odorant-binding protein domain (PBP-GOBP), except OBP8, which contained two of these conserved domains. An unrooted phylogenetic tree was constructed to show the relationships among these 10 BdorOBPs and the OBPs belonging to the same OBP family in other insects. We found significantly different transcript expression profiles in each OBP gene at each stage in response to different treatments; these results revealed that OBP2 expression was significantly increased in response to baits at each adult physiological stage, while OBP2, OBP5 and OBP1 were highly expressed in response to combined-volatiles treatment at all tested physiological stages. OBP3, OBP5 and OBP10 showed high expression in response to irradiation at all tested physiological stages. Thus, we infer that protein baits, brewer's yeast volatiles and irradiation significantly influence the transcript levels of OBP genes, which may act in olfactory perception.

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Identification and Expression Profile Analysis of Odorant Binding Proteins in the Oriental Fruit Fly Bactrocera dorsalis

Cited by 59 publications

References 40 publications

Sniffing Out Chemosensory Genes from the Mediterranean Fruit Fly, Ceratitis capitata

Sniffing Out Chemosensory Genes from the Mediterranean Fruit Fly, Ceratitis capitata

Novel odorant-binding proteins and their expression patterns in grasshopper, Oedaleus asiaticus

Protein Baits, Volatile Compounds and Irradiation Influence the Expression Profiles of Odorantbinding Protein Genes in Bactrocera Dorsalis (Diptera: Tephritidae)

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