Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, <i>Aphis glycines</i> (Hemiptera: Aphididae)

Bi, Ying‐Dong; Liu, Ming; Li, Wei; Liu, Miao; Di, Shu‐Feng; Yang, Shuai; Fan, Chao; Bai, Lei; Lai, Yongcai

doi:10.1111/1744-7917.12709

Cited by 15 publications

(18 citation statements)

References 39 publications

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“…Furthermore, the antennal expression level of OBP7 in the winged aphids at 0 or 2 h (before the peak at 1 day) was the same as that in the wingless aphids during the whole adult stage after 1 day. Thus, once samples at these two time points were selected, it was not surprising that OBP7 expression was not significantly different between winged and wingless aphids, as previously reported in some studies (e.g., Xue et al, 2016;Wang et al, 2019). As after emergence, most OBP genes undergo a process of increased expression and then stabilized, and the time to stabilize was about 1-3 days after emergence (Figure 1).…”

Section: Discussionsupporting

confidence: 67%

“…The temporal expression profile of aphids has mainly been compared between the nymph and adult stages (e.g., Xue et al, 2016 ; Wang et al, 2019 ). The expression profiles revealed higher levels of some OBPs in the nymph stage than in adults.…”

Section: Introductionmentioning

confidence: 99%

“…Besides, they occur in non-sensory tissues and organs such as the wings (Calvello et al, 2003;Pelosi et al, 2005), reproductive organs (Li et al, 2008;Sun et al, 2012a), mandibular glands (Iovinella et al, 2011) and salivary glands (Zhang et al, 2017) as well. Work on OBPs in aphids has also been widely published, including studies focusing on expression analysis between winged and wingless morphs (e.g., Gu et al, 2013;Xue et al, 2016;Wang et al, 2019Wang et al, , 2021. For example, Wang et al (2021) conducted a systematic and detailed investigation of OBP expression in the antennae, head, wings, legs, cornicles, caudae, and thorax of the soybean aphid A. glycines through differential transcriptome analysis and quantitative real-time PCR (RT-qPCR).…”

Section: Introductionmentioning

confidence: 99%

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Novel Temporal Expression Patterns of EBF-Binding Proteins in Wing Morphs of The Grain Aphid Sitobion miscanthi

Zhang

Jiang

et al. 2021

Front. Physiol.

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High chemosensitivity of insects to volatile organic compounds (VOC) stimuli is mediated by odorant binding proteins (OBPs). In aphids, three OBPs (OBP3, OBP7 and OBP9) are E-β-farnesene (EBF)-binding proteins. Winged aphids are generally more sensitive than wingless aphids to VOCs, thus, wing presence is a phenotypic correlate of olfaction sensitivity. Here, we investigate the detailed temporal expression of these EBF-binding proteins and two other OBPs (OBP6 and OBP10), in the grain aphid Sitobion miscanthi 0 h, 2 h, 1 day, 3 days, 10 days, and 20 days after adult emergence. Both winged and wingless aphids were examined to further uncover phenotypic specification. Then, the expression patterns before and after EBF induction were analyzed. Throughout adulthood, only OBP7 had significantly higher antennal expression in winged aphids; however, there was no significant difference in the antennal expression of OBP3 between wing morphs at most time points. Except it was lower in newly emerged winged aphids but increased rapidly to the same level in wingless aphids at 1 day. OBP9 did not differ in expression between the morphs and was the only OBP that did not exhibit an expression trough at the beginning of the adult stage (0 h). The expression of OBP9 remained relatively stable and high throughout the adult stage in both phenotypes, showing the highest level among the three EBF-binding proteins. After EBF induction, its expression was further up-regulated in both morphs. Therefore, this protein may be an important molecule for EBF recognition in aphids. OBP7 strongly responded to EBF but only in winged aphids, suggesting that this protein is important in the more sensitive EBF recognition process of winged aphids. In addition, the antennal expression level of OBP3 did not respond to EBF induction. These findings revealed a temporal expression pattern of OBPs in aphids and showed that figuring out the pattern is critical for correctly selecting morphs and sampling times, which will support the discovery of reliable findings and allow solid conclusions to be drawn. Our findings also inspire on the interaction mode of the three EBF-binding proteins in relation to EBF perception in aphids.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel Temporal Expression Patterns of EBF-Binding Proteins in Wing Morphs of The Grain Aphid Sitobion miscanthi

Zhang

Jiang

et al. 2021

Front. Physiol.

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“…As one of the most important groups of chemo-reception proteins in insects, OBPs have been studied since 1981 (Vogt and Riddiford, 1981). Regarding aphids, OBPs have been widely reported for Acyrthosiphon pisum (Ji et al, 2016) genes encoding complete OBPs with a signal peptide, Zhou et al, 2010), Myzus persicae (Ji et al, 2016), Megoura viciae (Iovinella et al, 2011;Daniele et al, 2018), Sitobion avenae (Kim et al, 2013;Xue et al, 2016), Aphis gossypii (Grabherr et al, 2011;Gu et al, 2013), and Aphis glycines (Grabherr et al, 2011;Wang et al, 2019). Consistent with the simple facultative parasitic lifestyle of aphids, the aphid OBP family has few members, and they are generally more highly conserved than in other insects.…”

Section: Introductionmentioning

confidence: 99%

A Detailed Spatial Expression Analysis of Wing Phenotypes Reveals Novel Patterns of Odorant Binding Proteins in the Soybean Aphid, Aphis glycines

et al. 2021

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The wide range of insect niches has led to a rapid expansion of chemosensory gene families as well as their relatively independent evolution and a high variation. Previous studies have revealed some functions for odorant-binding proteins (OBPs) in processes beyond olfaction, such as gustation and reproduction. In this study, a comparative transcriptomic analysis strategy was applied for the soybean aphid, Aphis glycines, focusing on various functional tissues and organs of winged aphids, including the antenna, head, leg, wing, thorax, cauda, and cornicle. Detailed spatial OBP expression patterns in winged and wingless parthenogenetic aphids were detected by RT-qPCR. Twelve OBPs were identified, and three new OBPs in A. glycines are first reported. All OBPs showed comparatively higher expression in sensory organs and tissues, such as the antenna, head, or leg. Additionally, we found some novel expression patterns for aphid OBPs (Beckendorf et al., 2008). Five OBPs exhibited high-expression levels in the cauda and four in the cornicle (Biasio et al., 2015). Three genes (OBP2/3/15) were highly expressed in the wing (Calvello et al., 2003). Two (OBP3/15) were significantly more highly expressed in the wingless thorax than in the winged thorax with the wings removed, and these transcripts were significantly enriched in the removed wings. More details regarding OBP spatial expression were revealed under our strategy. These findings supported the existence of carrier transport functions other than for foreign chemicals and therefore broader ligand ranges of aphid OBPs. It is important for understanding how insect OBPs function in chemical perception as well as their other potential physiological functions.

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“…In Batocera horsfieldi Hope, BhorOBP1, BhorOBP2, and BhorOBP3 are expressed in the antennae, but also in the wings, legs, abdomens, maxillary palps, and labial palps (Li et al, 2012). These differences in distribution may be related to their specific features (He et al, 2011;Ma et al, 2016;Wang et al, 2019). Previous studies have shown that OBPs are expressed selectively in different types of sensilla on the antenna, which are considered the minimum functional units for chemoreception.…”

Section: Introductionmentioning

confidence: 99%

Functional Analysis of Two Odorant-Binding Proteins, MaltOBP9 and MaltOBP10, in Monochamus alternatus Hope

Huang

Yang

et al. 2020

Front. Physiol.

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Odorant-binding proteins (OBPs) are important for the perception of chemical signals by insects. Effective pest management strategies can be developed by understanding the host location mechanism and the physiological functions of OBPs in olfactory detection. In this study, we cloned two OBPs from Monochamus alternatus, where MaltOBP9 was highly expressed in multiple insect tissues and MaltOBP10 was highly expressed in the female antenna according to the results of qRT-PCR. The recombinant proteins were successfully purified in vitro. Immunocytochemistry indicated the high expression of MaltOBP9 and MaltOBP10 in the sensillum lymph of sensilla basiconica, sensilla trichodea, sensilla auricillica, and sensilla chaetica, thereby demonstrating their broad participation in semiochemical detection. Both proteins were localized in the inner cavity of mechanoreceptors and they exhibited broad binding abilities with volatiles from pine bark according to fluorescence competitive binding assays. Due to its broad binding ability and distribution, MaltOBP9 may be involved in various physiological processes as well as olfactory detection. MaltOBP10 appears to play a role in the fundamental olfactory recognition process of female adults according to its broad binding ability. These findings suggest that OBPs may have various physiological functions in insects, thereby providing novel insights into the olfactory receptive mechanism.

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Identification and expression profiles analysis of odorant‐binding proteins in soybean aphid, Aphis glycines (Hemiptera: Aphididae)

Cited by 15 publications

References 39 publications

Novel Temporal Expression Patterns of EBF-Binding Proteins in Wing Morphs of The Grain Aphid Sitobion miscanthi

Novel Temporal Expression Patterns of EBF-Binding Proteins in Wing Morphs of The Grain Aphid Sitobion miscanthi

A Detailed Spatial Expression Analysis of Wing Phenotypes Reveals Novel Patterns of Odorant Binding Proteins in the Soybean Aphid, Aphis glycines

Functional Analysis of Two Odorant-Binding Proteins, MaltOBP9 and MaltOBP10, in Monochamus alternatus Hope

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