Identification and function analyses of senescence-associated WRKYs in wheat

Zhang, Haoshan; Zhao, Mingming; Song, Qiuhang; Zhao, Lei; Wang, Geng; Zhou, Chunjiang

doi:10.1016/j.bbrc.2016.05.034

Cited by 29 publications

(27 citation statements)

References 33 publications

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“…A total of 160 TaWRKYs were characterized according to their HMM profiles, conserved domains, distribution among WRKY groups, and phylogenetic relationships, and some drought responsive members were validated in leaf and root tissues (Okay, Derelli & Unver, 2014). Recently, Zhang et al (2016) identified 116 WRKYs, and 13 of them were characterized as senescence-associated genes.…”

Section: Discussionmentioning

confidence: 99%

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Genome-wide analysis of WRKY transcription factors in wheat (Triticum aestivum L.) and differential expression under water deficit condition

Ning

Liu

Kang

et al. 2017

PeerJ

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Background. WRKY proteins, which comprise one of the largest transcription factor (TF) families in the plant kingdom, play crucial roles in plant development and stress responses. Despite several studies on WRKYs in wheat (Triticum aestivum L.), functional annotation information about wheat WRKYs is limited. Results. Here, 171 TaWRKY TFs were identified from the whole wheat genome and compared with proteins from 19 other species representing nine major plant lineages. A phylogenetic analysis, coupled with gene structure analysis and motif determination, divided these TaWRKYs into seven subgroups (Group I, IIa-e, and III). Chromosomal location showed that most TaWRKY genes were enriched on four chromosomes, especially on chromosome 3B. In addition, 85 (49.7%) genes were either tandem (5) or segmental duplication (80), which suggested that though tandem duplication has contributed to the expansion of TaWRKY family, segmental duplication probably played a more pivotal role. Analysis of cis-acting elements revealed putative functions of WRKYs in wheat during development as well as under numerous biotic and abiotic stresses. Finally, the expression of TaWRKY genes in flag leaves, glumes, and lemmas under water-deficit condition were analyzed. Results showed that different TaWRKY genes preferentially express in specific tissue during the grain-filling stage. Conclusion. Our results provide a more extensive insight on WRKY gene family in wheat, and also contribute to the screening of more candidate genes for further investigation on function characterization of WRKYs under various stresses.

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Section: Discussionmentioning

confidence: 99%

“…Okay, Derelli & Unver (2014) characterized 160 TaWRKYs and their expression profiling in RNA-Seq libraries. Recently, Zhang et al (2016) identified 116 WRKYs, and 13 of them were characterized as senescence-associated genes. Here, we provide extensive insights on TaWRKYs based on the whole genome sequence of wheat.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of WRKY transcription factors in wheat (Triticum aestivum L.) and differential expression under water deficit condition

Ning

Liu

Kang

et al. 2017

PeerJ

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“…Wheat Triticum aestivum [13,26] Table 2. Senescence-regulating WRKYs in important model and crop plant species 1 .…”

Section: Plant Species Referencesmentioning

confidence: 99%

Arabidopsis WRKY53, a Node of Multi-Layer Regulation in the Network of Senescence

Zentgraf

Doll

2019

Plants

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Leaf senescence is an integral part of plant development aiming at the remobilization of nutrients and minerals out of the senescing tissue into developing parts of the plant. Sequential as well as monocarpic senescence maximize the usage of nitrogen, mineral, and carbon resources for plant growth and the sake of the next generation. However, stress-induced premature senescence functions as an exit strategy to guarantee offspring under long-lasting unfavorable conditions. In order to coordinate this complex developmental program with all kinds of environmental input signals, complex regulatory cues have to be in place. Major changes in the transcriptome imply important roles for transcription factors. Among all transcription factor families in plants, the NAC and WRKY factors appear to play central roles in senescence regulation. In this review, we summarize the current knowledge on the role of WRKY factors with a special focus on WRKY53. In contrast to a holistic multi-omics view we want to exemplify the complexity of the network structure by summarizing the multilayer regulation of WRKY53 of Arabidopsis.

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“…For example, the Arabidopsis thaliana wrky53 mutant displays delayed leaf senescence, while its over-expression results in precocious senescence, demonstrating that WRKY53 is positively involved in leaf senescence [19,20]. Besides WRKY53, other well-characterized WRKYs that are involved in leaf senescence include WRKY6, WRKY22, WRKY33, WRKY53, WRKY54, WRKY70, and WRKY75 from Arabidopsis thaliana [3,9,11,12,21], OsWRKY23, OsWRKY42, OsWRKY80, and OsWRKY26 from rice [22,23,24], and TaWRKY7 from wheat [25]. To date, however, little is known about the molecular aspects of senescence-associated WRKY TFs in non-model plants, especially in economically important leafy vegetables.…”

Section: Introductionmentioning

confidence: 99%

BrWRKY65, a WRKY Transcription Factor, Is Involved in Regulating Three Leaf Senescence-Associated Genes in Chinese Flowering Cabbage

Fan

Tan

Shan

et al. 2017

IJMS

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Plant-specific WRKY transcription factors (TFs) have been implicated to function as regulators of leaf senescence, but their association with postharvest leaf senescence of economically important leafy vegetables, is poorly understood. In this work, the characterization of a Group IIe WRKY TF, BrWRKY65, from Chinese flowering cabbage (Brassica rapa var. parachinensis) is reported. The expression of BrWRKY65 was up-regulated following leaf chlorophyll degradation and yellowing during postharvest senescence. Subcellular localization and transcriptional activation assays showed that BrWRKY65 was localized in the nucleus and exhibited trans-activation ability. Further electrophoretic mobility shift assay (EMSA) and transient expression analysis clearly revealed that BrWRKY65 directly bound to the W-box motifs in the promoters of three senescence-associated genes (SAGs) such as BrNYC1 and BrSGR1 associated with chlorophyll degradation, and BrDIN1, and subsequently activated their expressions. These findings demonstrate that BrWRKY65 may be positively associated with postharvest leaf senescence, at least partially, by the direct activation of SAGs. Taken together, these findings provide new insights into the transcriptional regulatory mechanism of postharvest leaf senescence in Chinese flowering cabbage.

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Identification and function analyses of senescence-associated WRKYs in wheat

Cited by 29 publications

References 33 publications

Genome-wide analysis of WRKY transcription factors in wheat (Triticum aestivum L.) and differential expression under water deficit condition

Genome-wide analysis of WRKY transcription factors in wheat (Triticum aestivum L.) and differential expression under water deficit condition

Arabidopsis WRKY53, a Node of Multi-Layer Regulation in the Network of Senescence

BrWRKY65, a WRKY Transcription Factor, Is Involved in Regulating Three Leaf Senescence-Associated Genes in Chinese Flowering Cabbage

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