Identification and function of two myeloid differentiation factor 88 variants in triangle-shell pearl mussel (Hyriopsis cumingii)

Genome-wide identification and characterization of five MyD88 duplication genes in Yesso scallop (Patinopecten yessoensis) and expression changes in response to bacterial challenge

“…Therefore, a comprehensive understanding of this process will pave the way for understanding the mechanism of the innate immune response in mollusks and developing methods to control species-related diseases. MyD88s are pivotal adaptors in the TLR/IL-1R signaling pathway, and although they have been identified in several mollusks [18,19,45], the complete family of these genes and their immune-related regulatory functions remain mostly unclear in scallops. In this study, we analyzed the genome and transcriptome of an ancient marine bivalve, P. yessoensis, and successfully identified five MyD88 genes.…”

“…The tissue expression patterns of MyD88 genes have been widely characterized in various species, and high expression has been detected in multiple immune-related tissues [18,25,45], reflecting the crucial roles of these proteins in the host immune response. In this study, the ubiquitous expression of all PyMyD88s was observed in most healthy tissues, with higher abundance also detected in tissues relevant to the innate immune system.…”

“…A Nanovue Plus spectrophotometer (GE Healthcare, NJ, USA) was used to assess the concentration and purity of RNA; RNA integrity was determined by agarose gel electrophoresis. First-strand cDNA was synthesized according to the manufacturer's protocol using Moloney murine leukemia virus (MMLV) reverse transcriptase (Thermo, USA) in a 20 mL volume with 2 mg of total RNA as the template and 0.5 mg of oligo (dT) 18 (TaKaRa Biotechnology, Liaoning, China) as the primer. The mixture was denatured at 65 C for 5 min and then chilled immediately on ice.…”

Two myeloid differentiation factor 88 (MyD88) isoforms identified in ducks

“…Chicken MyD88-3 has an 8-amino acid deletion at the N-terminus compared to wild chicken MyD88-1 (Wheaton et al, 2007). In invertebrates, MyD88 variants are found in shrimp (Zhang et al, 2012) and pearl mussels (Ren et al, 2014).…”

“…MyD88 was first identified in 1990 (Lord et al, 1990) and has been studied in species from complex vertebrates to simple invertebrates including humans (Bonnert et al, 1997), mice (Hardiman et al, 1997), pigs (Tohno et al, 2007, reptiles , chickens (Wheaton et al, 2007), fish (Kongchum et al, 2011;Takano et al, 2006;van der Sar et al, 2006;Yao et al, 2009), scallops (Qiu et al, 2007;Ren et al, 2014), vannamei (Zhang et al, 2012), and fruitflies (Tauszig-Delamasure et al, 2002). Recently, a partial sequence of goose MyD88 was reported (Wei et al, 2013).…”