Identification and Functional Analysis of Differentially Expressed Genes Related to Metastatic Osteosarcoma

Niu, Feng; Zhao, Song; Xu, Changyan; Chen, Lin; Ye, Long; Bi, Gui‐Bin; Tian, Gang; Gong, Ping; Nie, Tian‐Hong

doi:10.7314/apjcp.2014.15.24.10797

Cited by 6 publications

(4 citation statements)

References 50 publications

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“…Of these differentially expressed lncRNAs, A2M-AS1, a novel lncRNA, was upregulated in breast cancer tissues and was associated with poor prognosis. A previous study reported that A2M upregulation is related to metastasis in osteosarcoma and to ovarian carcinogenesis [20,21]. A2M-AS1, the antisense of A2M, is located on the forward strand of chromosome 12 at positions 9,065,177-9,068,684 and may be similar to A2M.…”

Section: Discussionmentioning

confidence: 98%

Screening of a Novel Upregulated lncRNA, A2M-AS1, That Promotes Invasion and Migration and Signifies Poor Prognosis in Breast Cancer

Fang

Zhang

et al. 2020

BioMed Research International

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Understanding of prognostic factors and therapeutic targets for breast cancer is imperative for guidance of patient care. We studied 1203 tumour samples from the Gene Expression Omnibus (GEO) to evaluate potential genes related to breast cancer. R software was used to analyse differentially expressed long noncoding RNAs (lncRNAs) in the RNA microarray expression profiles GSE45827 and GSE65216 and to identify a series of differentially expressed lncRNAs associated with human breast cancer. Of these lncRNAs, A2M-AS1, a lncRNA that has not been previously reported, was significantly upregulated in human breast cancer tissues compared with adjacent nontumour tissues. Importantly, A2M-AS1 upregulation was significantly associated with ERnegative, HER2-positive, and basal-like breast cancer and with poor recurrence-free survival and metastasis-free survival in breast cancer patients. After validating these results in 96 collected human breast cancer tissues and 64 paired adjacent noncancerous tissues, we further investigated the roles of A2M-AS1 in human ER-negative and basal-like breast cancer cells. e results revealed that A2M-AS1 significantly promotes human breast cancer cell proliferation, invasion, and migration. Additionally, bioinformatics analysis of genes coexpressed with A2M-AS1 in the context of human breast cancer combined with qRT-PCR and Western blot assays revealed that A2M-AS1 exerts regulatory effects on downstream factors in the cell adhesion molecule pathway, including CD2 and SELL. ese results imply that A2M-AS1 might be a promising candidate prognostic factor and therapeutic target for breast cancer.

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Section: Discussionmentioning

confidence: 98%

Screening of a Novel Upregulated lncRNA, A2M-AS1, That Promotes Invasion and Migration and Signifies Poor Prognosis in Breast Cancer

Fang

Zhang

et al. 2020

BioMed Research International

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“…On the other hand, in human primary and metastatic OSA, A2M was shown to be upregulated [36,37]. Liu et al suggested that A2M may act as oncogene and be consistently involved in the pathophysiological process of human osteosaroma [36], suggesting that, together with LRP1, it may be considered as potential therapeutic targets [37].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analyses Reveal the Role of Alpha-2-Macroglobulin in Canine Osteosarcoma Cell Migration

Wilk,

Michalak,

Owczarek

et al. 2024

IJMS

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Canine osteosarcoma (OSA) is an aggressive bone neoplasia with high metastatic potential. Metastasis is the main cause of death associated with OSA, and there is no current treatment available for metastatic disease. Proteomic analyses, including matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI TOF/TOF MS), are widely used to select molecular targets and identify proteins that may play a key role in primary tumours and at various steps of the metastatic cascade. The main aim of this study was to identify proteins differently expressed in canine OSA cell lines with different malignancy phenotypes (OSCA-8 and OSCA-32) compared to canine osteoblasts (CnOb). The intermediate aim of the study was to compare canine OSA cell migration capacity and assess its correlation with the malignancy phenotypes of each cell line. Using MALDI-TOF/TOF MS analyses, we identified eight proteins that were significantly differentially expressed (p ≤ 0.05) in canine OSA cell lines compared to CnOb: cilia- and flagella-associated protein 298 (CFAP298), general transcription factor II-I (GTF2I), mirror-image polydactyly gene 1 protein (MIPOL1), alpha-2 macroglobulin (A2M), phosphoglycerate mutase 1 (PGAM1), ubiquitin (UB2L6), ectodysplasin-A receptor-associated adapter protein (EDARADD), and leucine-rich-repeat-containing protein 72 (LRRC72). Using the Simple Western technique, we confirmed high A2M expression in CnOb compared to OSCA-8 and OSCA-32 cell lines (with intermediate and low A2M expression, respectively). Then, we confirmed the role of A2M in cancer cell migration by demonstrating significantly inhibited OSA cell migration by treatment with A2M (both at 10 and 30 mM concentrations after 12 and 24 h) in a wound-healing assay. This study may be the first report indicating A2M’s role in OSA cell metastasis; however, further in vitro and in vivo studies are needed to confirm its possible role as an anti-metastatic agent in this malignancy.

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“…It has been shown in several studies that the discovery of metastases at diagnosis, is an important factor determining the survival of patients with bone neoplasms (Garza et al, 1999;Zuniga et al, 2005;Yang et al, 2012;Choeprasert et al, 2014;Joo et al, 2015;Muscolo et al, 2015) and this behavior has been associated with various features such as high malignancy (Garza et al, 1999;Yang et al, 2012;Linares-Gonzalez et al, 2014;Joo et al, 2015), local recurrence, (Niu et al, 2012;Li et al, 2015) genetic and hormonal stimuli (Niu et al, 2014;Uribe-Rosales et al, 2014), unconventional age and the anatomical region of the primary tumor (Uribe- Rosales et al, 2014;Joo et al, 2015), history of pathologic fracture due to the primary tumor (Cortes-Rodriguez et al, 2010), no chemotherapy or improper surgical handling (Muscolo et al, 2009) and tumor size (Weber et al, 2002;Yang et al, 2012). In our study, in 2012, increased frequency of metastases at diagnosis was observed; however, to discuss this aspect, we need to collect information on the methods applied for detection of metastases.…”

Section: Discussionmentioning

confidence: 99%

“…OS has been classified in metastatic and non-metastatic tumor due to the high frequency of this condition at diagnosis (Niu et al, 2014). Previous studies have reported a frequency of metastases in a range of 15% in the US to 60% in Thailand (Zhu et al, 2013;Choeyprasert et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Association of Metastasis with Clinicopathological Data in Mexican Patients with Osteosarcoma, Giant Cell Tumor of Bone and Chondrosarcoma

Estrada-Villaseor¹,

Escamilla-Uribe²,

P³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Background: Bone tumors are neoplasias with a high overall mortality; one of the main factors that reduce survival is their high capacity to develop metastases. It has been reported that finding lung metastases at diagnosis of osteosarcoma (OS), chondrosarcoma (CS) and giant cell tumor of bone (GCTb) is quite common. In this study, we inquire the relationship of metastases caused by these tumors with different clinical and pathological aspects, in order to guide medical personnel in the diagnosis and opportune treatment of metastases or micro metastases. Materials and Methods: We collected data of 384 patients with clinical, radiological and histopathological diagnosis of OS, GCTb and CS that attended the National Rehabilitation Institute (INR) during 2006 to 2014. Chi-square and Fisher's exact tests were performed for data analysis. Results: In the three tumor types, the presence of metastases at diagnosis was variable (p=0.0001). Frequency of metastases was 36.7%, 31.7% and 13.2% for OS, CS and GCTb respectively. The average age had no significant difference (p>0.05) in relation to metastases, even so, patients with OS and GCTb and metastases, were older while patients with CS and metastases were younger, in comparison to patients without metastases. Males had a higher frequency of metastases (68.2%, p = 0.09) in contrast to CS and GCTb, in which the metastases was more frequent in women with 51.9% (p = 0.44) and 57.9% (p = 0.56) respectively. Broadly, metastasis was associated with primary tumors located in the femur (44.4%), followed by the tibia (15.6%); metastases was more frequent when primary tumor of GCTb and OS were in the same bones, but were located in the hip (26.3%) for CS. Conclusions: The frequency of metastases in OS, GCTb and CS is high in our population and is determined by different clinicopathological variables related to the kind of tumor. Further studies are needed in order to evaluate metastases subsequent to diagnosis and associations with survival and clinicopathological factors , as well as to determine the sensitivity and specificity of current methods of detection.

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Identification and Functional Analysis of Differentially Expressed Genes Related to Metastatic Osteosarcoma

Cited by 6 publications

References 50 publications

Screening of a Novel Upregulated lncRNA, A2M-AS1, That Promotes Invasion and Migration and Signifies Poor Prognosis in Breast Cancer

Screening of a Novel Upregulated lncRNA, A2M-AS1, That Promotes Invasion and Migration and Signifies Poor Prognosis in Breast Cancer

Proteomic Analyses Reveal the Role of Alpha-2-Macroglobulin in Canine Osteosarcoma Cell Migration

Association of Metastasis with Clinicopathological Data in Mexican Patients with Osteosarcoma, Giant Cell Tumor of Bone and Chondrosarcoma

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