Identification and functional study of osteosarcoma metastasis marker genes

Abstract

Abstract. To date, osteosarcoma metastasis genes, which are key for accurate initial diagnosis of the disease, have not been well identified. In the present study, osteosarcoma samples with and without metastasis were collected from 31 patients. Specific complementary DNA subtraction techniques were used to identify the osteosarcoma metastasis transcripts, which are responsible for the metastasis of osteosarcoma. The specific differentially expressed transcripts were identified by Basic Local Alignment Search Tool analysis and the results were validated by immunoblotting. Specifically, ezrin and β4 integrin were employed as markers to detect osteosarcoma metastasis in the initial stages. The results of the present study indicated that the two transcripts, ezrin and β4 integrin, were highly expressed in patients with osteosarcoma metastasis, and concluded that these were osteosarcoma metastasis genes. These results indicate that β4 integrin and/or ezrin may be used as a novel marker for the detection of osteosarcoma metastasis in the initial stages.

Tumor cell-targeted delivery of CRISPR/Cas9 by aptamer-functionalized lipopolymer for therapeutic genome editing of VEGFA in osteosarcoma

“…To investigate whether LC09 could facilitate the delivery of CRISPR/Cas9 plasmids into tumor cells, we examined the co-localization of Cy5-labeled CRISPR/Cas9 plasmids with OS markers (Ezrin) [63] in cytosections of orthotopic OS tissues and lung metastatic sites from mice administrated with different CRISPR/Cas9 formulations. We found numerous instances of co-localization of Cy5-labeled CRISPR/Cas9 plasmids in OS tissues and lung metastatic sites from mice when LC09-PPC-CRISPR/Cas9 was administered, whereas there were few instances of such overlapping staining in OS tissues and lung metastatic sites from mice administrated with PPC-CRISPR/Cas9 or Rd-PPC-CRISPR/Cas9 (Fig.…”

Velvet Antler compounds targeting major cell signaling pathways in osteosarcoma - a new insight into mediating the process of invasion and metastasis in OS

“…Wang's group screened for mutations in 339 cancer-related genes from 10 osteosarcoma patients through high-throughput sequencing and observed novel 85 mutinied genes in at least one patient, 39 mutinied genes in at least five patients (Table 2) [38]. In addition, 12 osteosarcoma metastasis genes have been identified from 31 patients by cDNA subtraction experiment (Table 3) [39]. Also, more genes were found by other studies which are related to osteosarcoma.…”

Identification of the miRNA-mRNA regulatory network of small cell osteosarcoma based on RNA-seq

“…Osteosarcoma (OS) is a common primary bone tumor in young adults and adolescents [ 1 ], which is characterized by a high metastatic potential: approximately 20–25% of newly diagnosed patients harbor detectable lung-related metastases [ 2 , 3 ]. Moreover, the distal femur and proximal tibia are frequent metastatic locations for OS.…”