Identification and genetic characterization of an isolate of bovine adenovirus 7 from the United States, a putative member of a new species in the genus Atadenovirus

Paim, Willian Pinto; Bauermann, Fernando V.; Kutish, G. F.; Pillatzki, Angela; Long, Craig; Ohnstad, Martha; Diel, Diego G.

doi:10.1007/s00705-021-05184-x

Cited by 4 publications

(9 citation statements)

References 17 publications

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“…These studies have reported that the BadV-7 and OAdV-7 predicted aa sequences of the DNA polymerase, penton base and hexon genes share sequence identity of 67, 84, and 86% respectively. Our data also supports a recent analysis highlighting interspecies variability within the fiber gene (39.56% aa sequence identity to OAdV-7) [ 4 ]. We also identified the BAdV-7 genome region displaying the highest levels of intraspecies nucleotide sequence variability of BadV-7 to be in the genome region between the genes E4.1 and RH5.…”

Section: Discussionsupporting

confidence: 91%

“…Phylogenetic analysis of the newly derived full-length sequence of BAdV-7 strain S427/18 agrees with previous phylogenetic analyses of sequences derived from the hexon, DNA polymerase and penton base genes [ 3 , 4 , 20 ]. These studies have reported that the BadV-7 and OAdV-7 predicted aa sequences of the DNA polymerase, penton base and hexon genes share sequence identity of 67, 84, and 86% respectively.…”

Section: Discussionsupporting

confidence: 85%

“…The recovery of the first full-length genome sequence of a European BAdV-7 strain (S427/18) directly from clinical material means that this is a true wild-type virus sequence without the possibility of mutations due to propagation in primary or transformed cells. Isolation of BAdV-7 has previously been restricted to primary bovine testicular cells, and bovine and ovine primary nasal turbinate cells [ 4 , 15 ]. We have extended this list of susceptible cells by isolating BAdV-7 in KOP-R cells, a finite bovine eosophagus cell line with readily detected CPE on day three-post-infection.…”

Section: Discussionmentioning

confidence: 99%

“…Bovine adenoviruses are members of the genera Mastadenovirus (BAdV-1, -2, -3, and -10) and Atadenovirus (BAdV-4, -5, 6, -7 and -8) and have a worldwide distribution [ 2 ]. Bovine adenovirus 7 (BAdV-7) is a proposed member of the genus Atadenovirus [ 3 , 4 ] and the ICTV have reported that BAdV-7 is related to members of the genus Atadenovirus, but has not yet approved a formal classification of this virus, presumably “Bovine Atadenovirus F”, as a new atadenovirus species, [ 1 ]. Formerly, the classification of bovine adenoviruses was based on serological reactivity and the designation was comprised of the natural host and a serial number (bovine adenovirus 1, 2 etc.)…”

Section: Introductionmentioning

confidence: 99%

“…However, the complete genome sequences of the “Fukuroi” strain and the derivate live attenuated vaccine strain “TS-GT were only recently reported [ 18 , 20 ]. This is augmented by a complete sequence for the SD18-74 strain which was isolated from pooled lung tissue from two 2-week-old calves from South Dakota, USA, that succumbed to acute enteric disease in 2018 [ 4 ]. In this study, we present the first molecular characterization of a complete genome sequence of a European BAdV-7 strain which was derived from a virus isolate obtained from a peracutely deceased calf in Germany.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany

Jesse

Ciurkiewicz

Siesenop³

et al. 2022

Virol J

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Bovine adenovirus 7 (BAdV-7) is an unclassified member of the genus Atadenovirus with a worldwide distribution and has been reported to induce clinical disease of varying severity in infected cattle, ranging from asymptomatic infections to severe enteric or respiratory disease. In this study, we used next-generation sequencing to obtain the first complete genome sequence of a European strain of BadV-7, from pooled spleen and liver tissue obtained from a deceased newborn Limousin calf. Histopathological analysis and electron microscopy showing systemic lesions in multiple organs with intranuclear amphophilic inclusions observed in endothelial cells in multiple peripheral tissues. Virus isolation was readily achieved from tissue homogenate using bovine esophagus cells (KOP-R), a strategy which should facilitate future in vitro or in vivo BAdV-7 studies. Phylogenetic analysis of available genome sequences of BAdV-7 showed that the newly identified strain groups most closely with a recent BAdV-7 strain, SD18-74, from the USA, confirming that this newly identified strain is a member of the Atadenovirus genus. The fiber gene was found to be highly conserved within BAdV-7 strains but was highly divergent in comparison to Ovine adenovirus 7 (OAdV-7) (39.56% aa sequence identity). Furthermore, we report a variable region of multiple tandem repeats between the coding regions of E4.1 and RH5 genes. In summary, the presented pathological and molecular characterization of this case suggests that further research into the worldwide molecular epidemiology and disease burden of BAdV-7 is warranted.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany

Jesse

Ciurkiewicz

Siesenop³

et al. 2022

Virol J

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Genome analysis of a novel avian atadenovirus reveals a possible horizontal gene transfer

Zheng,

Teng,

Jiang

et al. 2024

Virology

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Virus viability in spiked swine bone marrow tissue during above‐ground burial method and under in vitro conditions

Ebling

Paim

Turner

et al. 2022

Transbounding Emerging Dis

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The emergence of high consequence animal diseases usually requires managing significant mortality. A desirable aspect of any carcass management method is the ability to contain and inactivate the target pathogen. The above‐ground burial (AGB) technique was recently developed and proposed as an alternative carcass management method. Here, we investigate the tenacity of swinepox virus (SwPV), as a surrogate model for African swine fever virus (ASFV) in swine carcasses during the AGB process. For this, SwPV was inoculated intrafemorally in 90 adult swine carcasses, which were subsequently disposed under AGB conditions. Bone marrow samples were recovered periodically throughout 12 months and virus viability was assessed by virus isolation (VI), whereas the presence of SwPV DNA was evaluated by quantitative polymerase chain reaction (qPCR). Additionally, an in vitro study assessed the inactivation rate of SwPV, Senecavirus A (SVA), and bovine viral diarrhoea virus (BVDV). Viral suspensions were mixed with bone marrow material and maintained at 21–23°C for 30 days. Virus viability was assessed by VI and viral titration. In the field study, SwPV remained viable only in 11 (55%) bone marrow samples collected on day 7; only viral DNA (and not infectivity) was detected afterwards. SwPV inactivation was estimated to have occurred by day 11. The in vitro testing revealed a variable tenacity of the studied viruses. The viability period was estimated in 28, 80, and 118 days, respectively, for BVDV, SwPV, and SVA. Overall, these findings indicate that the AGB technique was effective in quickly inactivating SwPV. Additionally, the SwPV inactivation rate is comparable to ASFV under field studies and poses a potential model for preliminary ASFV inactivation studies with reduced biosecurity requirements. Moreover, this study contributes to understanding the inactivation kinetics of viruses under specific conditions, which is critical when designing and applying countermeasures in case of biosecurity breaches in sites managing animal mortality.

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Identification and genetic characterization of an isolate of bovine adenovirus 7 from the United States, a putative member of a new species in the genus Atadenovirus

Cited by 4 publications

References 17 publications

Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany

Molecular characterization of a bovine adenovirus type 7 (Bovine Atadenovirus F) strain isolated from a systemically infected calf in Germany

Genome analysis of a novel avian atadenovirus reveals a possible horizontal gene transfer

Virus viability in spiked swine bone marrow tissue during above‐ground burial method and under in vitro conditions

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