Identification and genetic mapping of pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides)

Wu, Hua Hsi; Liu, Ziji; Zhu, Jianqing; Xie, Chaojie; Yang, Tsomin; Zhou, Yifei; Duan, Xiayu; Sun, Qixin; Liu, Zhiyong

doi:10.1007/s00122-009-1031-4

Cited by 146 publications

(74 citation statements)

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“…Development of resistant germplasm to powdery mildew is based on interspecific hybridization and backcrossing (Murphy;Navarro;Leath, 2002;Navarro et EMARA, H. M. et al al., 2000). To date, 61 powdery mildew resistance genes, mapped to 43 loci (McIntosh;Yamazaki;Dubcovsky, 2008;He et al, 2009;Hua et al, 2009;Li;Fang;Zhang, 2009;Luo et al, 2009) have been identified and formally catalogued in wheat. However, some of the genes, derived from wild relatives, are difficult to use in wheat breeding because of linkage drag (Hospital, 2001).…”

Section: Introductionmentioning

confidence: 99%

Identification of Pm24, Pm35 and Pm37 in thirteen Egyptian bread wheat cultivars using SSR markers

Emara

Omar

El-Shamy³

et al. 2016

Ciênc. agrotec.

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Powdery mildew of wheat (Triticum spp.) caused by Blumeria graminis f.sp. tritici (DC) E.O. Speer Em. Marchal is one of the most important bread wheat diseases in Egypt. All the Egyptian common bread wheat cultivars are susceptible to that disease at seedling and adult stages. Breeding of resistant cultivars is the most economical and environmentally safe method to eliminate the disease and reduce crop losses. Combinations of two or more effective resistance genes may lead to better, more durable resistance to that disease. Eight Pm genes i.e. Pm2, Pm6, Pm12, Pm16, Pm24, Pm35, Pm36 and Pm37 out of 21 powdery mildew monogenic wheat lines (Pm) were resistant to 42 individual isolates of powdery mildew collected from different governorates in the Nile Delta area, Egypt, at seedling and adult stages. Only four DNA specific SSR markers (Xgwm337, Xcfd7 linked to Pm24, Pm35 and Xgwm332, Xwmc790) linked to Pm37 resistance genes were selected to detect these genes in 13 Egyptian common bread wheat cultivars. This study reveals the absence of Pm24, Pm35 and Pm37 in all the 13 Egyptian bread wheat cultivars. These results gave evidence that the Egyptian cultivars are not having resistance genes and need to further incorporate one, two or more resistant genes in a single genotype as all commercial cultivars defeated by the pathogen.

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Section: Introductionmentioning

confidence: 99%

Identification of Pm24, Pm35 and Pm37 in thirteen Egyptian bread wheat cultivars using SSR markers

Emara

Omar

El-Shamy³

et al. 2016

Ciênc. agrotec.

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“…MlZec1, a dominant resistance gene derived from wild emmer, was mapped distally to SSR marker Xwmc356 in terminal bin 2BL 0.89-1.00 (Mohler et al 2005). Two recessive powdery mildew resistance genes, Pm26 and Pm42 from wild emmer were located on chromosome 2BS (Rong et al 2000;Hua et al 2009). Our integrated mapping results indicate that MlIW170 is 17.15 cM distal to Pm42 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…dicoccoides (2n = 4x = 28; genome AABB), the progenitor of cultivated tetraploid and hexaploid wheats, is rich in genetic diversity for resistances to powdery mildew (Moseman et al 1984). Several powdery mildew resistance genes, such as Pm16 (Reader and Miller 1991), Pm26 (Rong et al 2000), Pm30 (Liu et al 2002), MlZec1 (Mohler et al 2005), MlIW72 (Ji et al 2007), Pm36 (Blanco et al 2008), Pm41 (Li et al 2009), Pm42 (Hua et al 2009), PmG16 (Ben-David et al 2010, and Ml3D232 were identified in wild emmer, or transferred to cultivated wheat or cultivated wheat derivatives.…”

Section: Introductionmentioning

confidence: 99%

Identification and comparative mapping of a powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides) on chromosome 2BS

et al. 2011

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Powdery mildew, caused by Blumeria graminis f. sp. tritici, is an important foliar disease of wheat worldwide. Wild emmer (Triticum turgidum var. dicoccoides) is a valuable genetic resource for improving disease resistance in common wheat. A powdery mildew resistance gene conferring resistance to B. graminis f. sp. tritici isolate E09 at the seedling and adult stages was identified in wild emmer accession IW170 introduced from Israel. An incomplete dominant gene, temporarily designated MlIW170, was responsible for the resistance. Through molecular marker and bulked segregant analyses of an F(2) population and F(3) families derived from a cross between susceptible durum wheat line 81086A and IW170, MlIW170 was located in the distal chromosome bin 2BS3-0.84-1.00 and flanked by SSR markers Xcfd238 and Xwmc243. MlIW170 co-segregated with Xcau516, an STS marker developed from RFLP marker Xwg516 that co-segregated with powdery mildew resistance gene Pm26 on 2BS. Four EST-STS markers, BE498358, BF201235, BQ160080, and BF146221, were integrated into the genetic linkage map of MlIW170. Three AFLP markers, XPaacMcac, XPagcMcta, XPaacMcag, and seven AFLP-derived SCAR markers, XcauG2, XcauG3, XcauG6, XcauG8, XcauG10, XcauG20, and XcauG25, were linked to MlIW170. XcauG3, a resistance gene analog (RGA)-like sequence, co-segregated with MlIW170. The non-glaucousness locus Iw1 was 18.77 cM distal to MlIW170. By comparative genomics of wheat-Brachypodium-rice genomic co-linearity, four EST-STS markers, CJ658408, CJ945509, BQ169830, CJ945085, and one STS marker XP2430, were developed and MlIW170 was mapped in an 2.69 cM interval that is co-linear with a 131 kb genomic region in Brachypodium and a 105 kb genomic region in rice. Four RGA-like sequences annotated in the orthologous Brachypodium genomic region could serve as chromosome landing target regions for map-based cloning of MlIW170.

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“…The sterility of the pentaploid hybrids is generally conquered by further crossing or backcrossing the hybrids with common wheat cultivars (AABBDD) (Liu et al, 2002, Brown-Guedira et al, 2005, Hua et al, 2009, Ogbonnaya and Abdalla, 2013. …”

Section: Genome Evolution and Domestication In Triticum Aestivummentioning

confidence: 99%

Genome diversity in Triticum aestivum

Lai¹

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The aims of this PhD research are to identify and characterise genome diversity in Triticum aestivum (bread wheat).This research will establish a process for the identification of large numbers of single nucleotide polymorphisms (SNPs) and other genetic variations in Triticum aestivum.Single nucleotide polymorphisms (SNPs) are the most abundant type of molecular genetic marker and can be used for producing high-resolution genetic maps, marker-trait association studies and marker assisted breeding. Large polyploid genomes, such as wheat, present a challenge for SNP discovery due to the potential presence of multiple homoeologues for each gene.AutoSNPdb has been successfully applied to identify SNPs from Sanger sequence data for several species, including barley, rice and Brassica, but the volume of data required to accurately call SNPs in the complex genome of wheat has prevented its application to this important crop. DNA sequencing technology has been revolutionised by the introduction of next generation sequencing, and it is now possible to generate several million sequence reads in a timely and cost effective manner.Wheat transcriptome sequence data has been generated using Roche 454 Life Sciences technology. This data has been applied for SNP discovery using a modified autoSNPdb method, which integrates SNP and gene annotation information with a graphical viewer. A total of 4,694,141 sequence reads from three bread wheat varieties were assembled to identify a total of 38,928 candidate SNPs. Each SNP is within an assembly complete with annotation, enabling the selection of polymorphism within genes of interest.The discovery of large numbers of genomic SNPs across 16 Australian diverse bread wheat varieties has been completed using a novel algorithm SGSautoSNP. More than 10x whole genome shotgun Illumina paired read sequence data was generated through a bioplatforms collaboration and the data mapped to the draft assemblies of chromosomes ii 7A, 7B and 7D. Over 4 million inter-varietal SNPs were identified. SNP density varied along the lengths chromosome syntenic builds as well as between genomes.SNP density analysis and SNP transition/transversion ratio analysis provide insights into the evolution and breeding history of this important crop. Both the SNP density and SNP transition/transversion ratios across the D genome are significantly lower than across the A and B genomes. This difference reflects the evolutionary history of this crop. In addition, genes within low SNP density regions may have been selected during domestication and breeding. Furthermore, this SNP resource permits the application of high resolution skim based genotyping by sequencing (GBS), trait association and analysis of structural variation in populations.An integrated database and portal for wheat genome resource, WheatGenome.info, has been established and published online. This portal provides a variety of web-based systems hosting wheat genome and genomic data, including genomic SNPs, to support wheat research and crop improve...

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Identification and genetic mapping of pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides)

Cited by 146 publications

References 23 publications

Identification of Pm24, Pm35 and Pm37 in thirteen Egyptian bread wheat cultivars using SSR markers

Identification of Pm24, Pm35 and Pm37 in thirteen Egyptian bread wheat cultivars using SSR markers

Identification and comparative mapping of a powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides) on chromosome 2BS

Genome diversity in Triticum aestivum

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