Identification and Genetic Variation among Hibiscus Species (Malvaceae) Using RAPD Markers

Abstract

Germplasm identification and characterization is an important link between the conservation and utilization of plant genetic resources. Traditionally, species or cultivars identification has relied on morphological characters like growth habit or floral morphology like flower colour and other characteristics of the plant. Studies were undertaken for identification and determination of genetic variation within the two species of Hibiscus and 16 varieties of Hibiscus rosa-sinensis L. through random amplified polymorphic (RAPD) markers. Primer screening was made by using the DNA of variety "Prolific". Genetic analysis was made by using ten selected decamer primers. A total of 79 distinct DNA fragments ranging from 0.3 to 2.5 kb were amplified by using ten selected random decamer primers. The genetic similarity was evaluated on the basis of presence or absence of bands. The cluster analysis indicated that the 16 varieties and two species formed one cluster. The first major cluster consisted of three varieties and a second major cluster consisted of two species and 13 varieties. The genetic distance was very close within the varieties and also among the species. Thus, these RAPD markers have the potential for identification of species/varieties and characterization of genetic variation within the varieties. This is also helpful in Hibiscus breeding programs and provides a major input into conservation biology.

Variability of esterase patterns in adult flies of the saltans species group of Drosophila (subgenus Sophophora)

“…Thus, additional markers for identification may be useful and have been developed in several organisms. Most of them are specific DNA or RNA sequences obtained by different techniques (Li et al 2002;Randig et al 2004;Barik et al 2006;Vargas et al 2005;Bianchi et al 2004). Speciesmarker bands in native and SDS-PAGE gel electrophoresis of soluble proteins were described in fish by Hasnain et al (2005).…”

Evaluation of Secondary Metabolites in Wheat Grain (Triticum Sp.) Grown in Humid South Eastern Plain Zone of Rajasthan (India)

“…Neighbour-joining tree of 126 individuals of petunia (coloured by taxon), constructed based on a matrix of microsatellite alleles shared among individuals (Turchetto et al 2015). Barik et al (2006) also found the genetic variation within the two species of Hibiscus and 16 varieties of Hibiscus rosa-sinensis L. through random amplified polymorphic (RAPD) markers. However, cluster analysis indicated that 16 varieties and two species formed one cluster.…”

Phylogenic Study of Twelve Species of Phyllanthus Originated from India through Molecular Markers for Conservation

“…Both RAPD and IS-SR marker, based on PCR techniques have proven to be a reliable, easy to generate, inexpensive and versatile set of marker that rely on repeatable amplification of DNA se-quence using single primers. The RAPD and ISSR markers can be used in the study of the genetic variability of species or natural populations and in the identification of genotypes [7][8][9][10][11][12][13][14]. In this communication, we report the feasibility of PCR-based DNA (RAPD and ISSR) marker for phylogeny study and identification for conservation of Phyllanthus species.…”