Identification and genotyping of molluscum contagiosum virus from genital swab samples by real-time PCR and Pyrosequencing

Orthopox Viruses: Infections in Humans

“…1). PCR and sequence analysis can be used for a differential diagnosis and genetic subtyping [81,86].…”

Orthopockenviren: Infektionen des Menschen. Stellungnahmen des Arbeitskreises Blut des Bundesministeriums für Gesundheit

“…1). Für die Differentialdiagnostik und die Subtypisierung von MOCV können PCR und Sequenzanalyse eingesetzt werden [81,86].…”

“…Frühere Beiträge befassten sich mit der Creutzfeldt-Jakob-Erkrankung, dem Parvovirus B19 und dem GB-Virus Typ C (HepatitisGVirus), (Bundes gesundheitsbl., 41,[78][79][80][81][82][83][84][85][86][87][88][89][90]1998), HTLV-I/-II, (Bundesgesundheitsbl., 41,512,1998), Yersinia enterocolitica, (Bundesgesundheitsbl., 42,613,1999), TT-Virus (Bundesgesundheitsbl., 43,[154][155][156]2000), Hepatitis B Virus (HBV (Bundesgesundheitsbl., 43,[240][241][242][243][244][245][246][247][248]2000). Humanes Cytomegalovirus (HCMV), (Bu ndesgesund heitsbl., 43,[653][654][655][656][657][658][659]2000), Hepatitis A Virus (Bundesgesundheitsbl., 44,[844][845][846][847][848][849][850]…”

“…4 6 , 7 1 2 -7 2 2 , 2 0 0 3), Hu m a n e s Immunschwächevirus (HIV) (Bundesge sundheitsbl. 47, [83][84][85][86][87][88][89][90][91][92][93][94][95]2004 …”

Emerging Molecular Assays for Detection and Characterization of Respiratory Viruses

“…In addition, pyrosequencingbased assays have been reported for the characterization, quantification, typing, subtyping, and drug-resistance profiling of other viruses. [107][108][109][110][111][112] One unique feature of pyrosequencing is its theoretical adaptability to the analysis of any genetic marker, which allows for the detection of multiple known and unknown mutations in a single pyrosequencing reaction. Integration of high-throughput pyrosequencing with the Roche/454 instrument has become a powerful tool for whole genome sequencing without the need for additional equipment or molecular techniques other than standard PCR, Genome Sequencer FLX sample preparation, and the sequencing pipeline.…”