Identification and In Silico Analysis of Lectins in Gray Oyster Culinary-Medicinal Mushroom Pleurotus ostreatus (Agaricomycetes) Based on the Transcriptomes

Identification and physiological function of one microRNA (<i>Po-MilR-1</i>) in oyster mushroom <i>Pleurotus ostreatus</i>

“…Four developmental stages of P. ostreatus , including mycelium (Myc), primordium (Pri, 2–3 mm diam), young fruiting body (Yfb, no gill formed), and mature fruiting body (Mfb, gills formed and spores dispersed), were produced and their RNA samples were harvested and stored at –80 °C as previously described ( Xu et al, 2019 ). Then expression level of Po-MilR-1 from four developmental stages were analyzed by quantitative real-time PCR (qRT-PCR) with SYBR Green Master Mix (VAZYME, Nanjing, China) using the ABI ViiA7 Real-Time PCR System (Applied Biosystems, Fosterusing City, USA).…”

“…The colony diameters were measured with three repetitions at the 6th day. To investigate the fruiting body formation, the mycelium of wild-type strain and the transformants were cultured on the 280 g sterile substrate bag (500 g/L cottonseed shell, 15 g/L bran, 2.5 g/L gypsum, 2.5 g/L lime and about 700 mL distilled water) in dark and developed to fruiting body as previously described ( Xu et al, 2019 ). The times when mycelium covered the bag and primordium formation were recorded.…”

“…Pleurotus ostreatus is an important edible and medicinal mushroom, and is a useful model for researching the mechanism of fruiting body development in mushrooms ( Sánchez, 2010 ; Fang et al, 2014 ; Gąsecka, Mleczek, Siwulski, & Niedzielski, 2016 ). In our previous work, miRNA-Seq and RNA-Seq were performed in four different developmental stages of P. ostreatus including the mycelium (Myc), primordium (Pri), young fruiting body (Yfb), and mature fruiting body (Mfb) ( Xu et al, 2019 ). After screening the differentially expressed miRNAs, one miRNA (named Po-MilR-1 ) was found to be barely expressed in Myc, but its expression increased dramatically in Pri with a |log 2 Pri/Myc| value of 16.04, and showed an increasing trend in the fruiting body, with the RPKM value of 2867.2 in Yfb and 10210.0 in Mfb, which suggested that it may play a potential role in fruiting body development.…”

Mycelium vs. Fruiting Bodies of Edible Fungi—A Comparison of Metabolites

“…No such changes were observed for most other enzymes [ 29 ]. The expression of genes coding for zinc finger proteins in P. ostreatus was regulated by developmental processes, phytoeffectors and abiotic stress [ 30 ], whereas, in line with a genetic study [ 13 ], the spectrum of lectins changed from mycelium to primordium to mature fruiting body [ 31 ]. A lentinan degrading β -1,3-glucanase of L. edodes occurred after the harvest of fruiting bodies but was not detectable in mycelia and young fruiting bodies [ 32 ].…”

“…They thrive on most of the substrates shown in Table 1 by quickly inducing large enzymatic networks (own experiments, data not shown). As a result, the composition of the fruiting bodies depends on the growth substrate [ 33 , 55 , 57 , 60 , 67 , 72 , 74 ], the strain chosen [ 33 , 60 , 74 , 75 , 76 , 77 , 78 , 80 ], its developmental stage [ 29 , 30 , 31 , 32 , 38 ], and the associated adverse or beneficial microbial community [ 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 ] and is thus variable like no other food. The same applies to the mass production of fungal mycelia.…”